FastQCFastQC Report
Thu 2 Feb 2017
SRR4062785_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062785_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1892400
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT48490.25623546818854365No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT33090.174857324032974No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT31950.16883322764743183No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC31180.16476432043965333No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA28390.15002113718030016No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG28030.14811879095328684No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG27180.1436271401395054No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC26270.13881843162122173No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC26150.1381843162122173No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG25910.1369160853942084No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT24010.12687592475163811No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG23290.1230712322976115No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG22940.12122172902134855No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA22900.12101035721834706No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA22090.11673007820756712No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG22030.11641302050306489No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC21700.11466920312830269No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC21170.11186852673853308No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG21120.11160431198478124No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC20780.10980765165926865No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT20510.10838089198900867No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT19060.10071866413020503No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG401.596853E-423.9938565
TAACGCA601.141145E-418.6613964
GTCCTAT8000.018.0172921
CTAGCGG6050.017.71868529
TAGCGGC6150.017.69073730
TCTAGCG6100.017.57252128
CCTATTC7950.017.102543
GTATTAT1900.016.8582861
CTATTCC8300.016.5736314
ATCGTTT5250.016.15208829
GCGCAAG7600.015.5939141
TATTCCA9200.015.4742995
CGGTCCA7250.015.448812510
AGCGGCG7100.015.32366631
TCCTATT9200.015.3077112
CGTTTAT5550.015.27900231
CATCGTT5650.015.00778428
CCGGTCC7500.014.9338519
GTATTAG5050.014.9053941
ATACCGT5700.014.8733846
Produced by FastQC (version 0.11.5)