##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062785_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1892400
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.263377192982457	32.0	32.0	32.0	32.0	32.0
2	30.874518600718662	32.0	32.0	32.0	32.0	32.0
3	30.927270661593745	32.0	32.0	32.0	32.0	32.0
4	30.98477277531177	32.0	32.0	32.0	32.0	32.0
5	30.908073874445147	32.0	32.0	32.0	32.0	32.0
6	34.56585869794969	36.0	36.0	36.0	32.0	36.0
7	34.49266117099979	36.0	36.0	36.0	32.0	36.0
8	34.47979074191503	36.0	36.0	36.0	32.0	36.0
9	34.60860071866413	36.0	36.0	36.0	32.0	36.0
10	34.331972098922	36.0	36.0	36.0	32.0	36.0
11	34.59675597125344	36.0	36.0	36.0	32.0	36.0
12	34.40520820122595	36.0	36.0	36.0	32.0	36.0
13	34.50145159585711	36.0	36.0	36.0	32.0	36.0
14	34.39821972098922	36.0	36.0	36.0	32.0	36.0
15	34.34282339885859	36.0	36.0	36.0	32.0	36.0
16	34.3689436694145	36.0	36.0	36.0	32.0	36.0
17	34.275056541957305	36.0	36.0	36.0	32.0	36.0
18	34.30642623124075	36.0	36.0	36.0	32.0	36.0
19	34.297111604311986	36.0	36.0	36.0	32.0	36.0
20	34.28879729444092	36.0	36.0	36.0	32.0	36.0
21	34.25938860705982	36.0	36.0	36.0	32.0	36.0
22	34.23849133375608	36.0	36.0	36.0	32.0	36.0
23	34.186572077784824	36.0	36.0	36.0	32.0	36.0
24	34.174184104840414	36.0	36.0	36.0	32.0	36.0
25	34.14209627985627	36.0	36.0	36.0	32.0	36.0
26	34.088932043965336	36.0	36.0	36.0	32.0	36.0
27	34.097154407102096	36.0	36.0	36.0	32.0	36.0
28	34.08590467131685	36.0	36.0	36.0	32.0	36.0
29	34.047295497780595	36.0	36.0	36.0	32.0	36.0
30	34.02115778905094	36.0	36.0	36.0	32.0	36.0
31	34.04939124920735	36.0	36.0	36.0	32.0	36.0
32	33.991007186641305	36.0	36.0	36.0	32.0	36.0
33	33.95629782287043	36.0	36.0	36.0	32.0	36.0
34	33.96460843373494	36.0	36.0	36.0	32.0	36.0
35	33.91671739589939	36.0	36.0	36.0	32.0	36.0
36	33.87746036778694	36.0	36.0	36.0	32.0	36.0
37	33.876764954555064	36.0	36.0	36.0	32.0	36.0
38	33.29141619108011	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	3.0
9	4.0
10	17.0
11	28.0
12	8.0
13	7.0
14	146.0
15	384.0
16	533.0
17	655.0
18	899.0
19	1122.0
20	1575.0
21	2346.0
22	3581.0
23	5585.0
24	8394.0
25	12739.0
26	18646.0
27	26204.0
28	37056.0
29	49315.0
30	65722.0
31	89193.0
32	120245.0
33	179988.0
34	401305.0
35	866695.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.52507334054615	16.827508855093896	11.148926939854139	25.49849086450582
2	16.997126998914034	19.488294926197604	36.811846450410854	26.702731624477504
3	19.101771438507	23.59249145567229	28.229338370175107	29.076398735645608
4	12.554634362616277	15.155946062917437	35.85356431913073	36.435855255335554
5	14.924307597352774	36.25699906362093	33.305502653778596	15.513190685247697
6	34.54676793505803	35.30052097947735	16.794044801381528	13.358666284083096
7	30.64404988374551	30.331748044810823	20.713538363982245	18.310663707461426
8	28.69714234984892	32.15123277710667	19.500273198921576	19.65135167412283
9	27.59265375357682	14.099329156967757	18.61297456397845	39.69504252547698
10	16.200757453657882	26.08263716891787	30.95947829018602	26.75712708723822
11	38.1935284767316	21.00830078125	21.69865860051407	19.09951214150433
12	25.149298727981705	23.770428996373287	28.1634393692066	22.916832906438405
13	29.539654176450657	18.93335574204821	25.22169565265646	26.305294428844668
14	23.9361955123564	19.751741078459247	24.80813521486425	31.503928194320103
15	25.460076978127518	26.84244854294422	22.243475834132326	25.453998644795945
16	26.187770848747487	25.613835746749814	23.385424373744847	24.81296903075785
17	24.33219521992368	25.665849198211436	24.88446210928003	25.117493472584858
18	25.54662402360729	24.149803122871305	26.031854407632625	24.27171844588878
19	25.681393845774974	24.859572754498455	25.270034102060972	24.188999297665596
20	25.92681648209254	23.738191518820642	24.914706249167672	25.420285749919145
21	27.587143551694254	23.90851248229649	24.142621599340476	24.36172236666878
22	26.073108346633127	24.002621204274256	24.87179351674717	25.052476932345446
23	24.263603458581212	23.766462796893176	25.55584152168272	26.414092222842893
24	24.811470978236915	24.97530585593418	25.06139844166606	25.151824724162843
25	24.989495703941106	24.281281529404367	25.050011019601147	25.679211747053383
26	24.78307770936826	25.324769018787684	25.485389556335313	24.406763715508735
27	25.678921525882963	24.43229651379405	24.62867866415746	25.260103296165525
28	24.541165379164127	24.44403459504707	25.74150739789906	25.27329262788975
29	24.429663069564008	24.803730741037676	25.722093827672023	25.04451236172629
30	24.44014993101123	25.01150693560397	25.982792705527608	24.565550427857193
31	25.1776164204029	24.985942883716998	24.366584226435833	25.46985646944427
32	24.85765803472436	24.664609882078388	24.30958730078202	26.168144782415233
33	24.384397207347227	24.19937946921418	25.25626885651005	26.159954466928543
34	25.461651274327163	24.574682574286204	25.333121936395948	24.630544214990685
35	26.19481626606473	24.14361525301634	25.394546091640503	24.26702238927843
36	24.502104787499704	25.243127399761644	24.834114205531904	25.42065360720675
37	25.754036537054965	25.05089270992318	24.77534277270671	24.41972798031514
38	24.633737622807487	24.558115004510405	25.337804809091203	25.470342563590908
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	212.0
1	232.0
2	252.0
3	252.0
4	658.0
5	1064.0
6	1064.0
7	1358.5
8	1653.0
9	1569.5
10	1486.0
11	1486.0
12	2024.5
13	2563.0
14	3285.0
15	4007.0
16	4007.0
17	6005.0
18	8003.0
19	8003.0
20	9673.5
21	11344.0
22	12057.5
23	12771.0
24	12771.0
25	14897.0
26	17023.0
27	17023.0
28	21870.5
29	26718.0
30	33212.0
31	39706.0
32	39706.0
33	51121.5
34	62537.0
35	62537.0
36	70257.5
37	77978.0
38	89552.0
39	101126.0
40	101126.0
41	107778.5
42	114431.0
43	130948.0
44	147465.0
45	147465.0
46	152077.5
47	156690.0
48	156690.0
49	166440.0
50	176190.0
51	178446.0
52	180702.0
53	180702.0
54	171316.0
55	161930.0
56	161930.0
57	157304.0
58	152678.0
59	136488.0
60	120298.0
61	120298.0
62	115747.5
63	111197.0
64	92646.5
65	74096.0
66	74096.0
67	61816.0
68	49536.0
69	49536.0
70	39772.0
71	30008.0
72	23489.0
73	16970.0
74	16970.0
75	12745.5
76	8521.0
77	8521.0
78	8541.5
79	8562.0
80	6569.0
81	4576.0
82	4576.0
83	4862.5
84	5149.0
85	5149.0
86	3449.5
87	1750.0
88	1483.5
89	1217.0
90	1217.0
91	864.0
92	511.0
93	442.0
94	373.0
95	373.0
96	326.0
97	279.0
98	279.0
99	553.5
100	828.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11863242443458043
2	0.05273726484886916
3	0.01104417670682731
4	0.0024836186852673853
5	2.1137180300147962E-4
6	1.585288522511097E-4
7	0.0
8	3.170577045022194E-4
9	2.6421475375184954E-4
10	3.699006552525893E-4
11	0.0025364616360177552
12	0.002800676389769605
13	0.014743183259353202
14	0.009617417036567322
15	0.022933840625660538
16	0.01056859015007398
17	0.020080321285140562
18	0.0054428239272881
19	0.007345170154301416
20	0.006129782287042908
21	0.0063411540900443885
22	0.007398013105051786
23	0.009458888184316214
24	0.013263580638342846
25	0.01675121538786726
26	0.019181991122384275
27	0.009036144578313253
28	0.00560135277953921
29	0.012101035721834708
30	0.003329105897273304
31	0.0063411540900443885
32	0.006658211794546608
33	0.007873599661805116
34	0.011889663918833228
35	0.0152716127668569
36	0.015060240963855423
37	0.009723102938068062
38	0.005759881631790319
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1892400.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.66795465519126
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.16234714579478	39.09658306271256
2	13.756766258651204	13.940544179908088
3	4.358609556668419	6.625254941308762
4	1.7866404161072627	3.6210166235381926
5	0.875592496664743	2.2182240458717453
6	0.5073625398701355	1.5424213298329614
7	0.3128298677273258	1.1095314686958335
8	0.21478475166385302	0.8706163246344495
9	0.1489208842131889	0.6790964947672358
>10	0.7080356715229389	6.393246864303999
>50	0.06436253825570398	2.3186673782976386
>100	0.07964577334063373	8.90466359487223
>500	0.01529959360826341	5.32163573409464
>1k	0.008802505911603606	7.3584979571616635
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4849	0.25623546818854365	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3309	0.174857324032974	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3195	0.16883322764743183	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3118	0.16476432043965333	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2839	0.15002113718030016	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2803	0.14811879095328684	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2718	0.1436271401395054	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2627	0.13881843162122173	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2615	0.1381843162122173	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2591	0.1369160853942084	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2401	0.12687592475163811	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2329	0.1230712322976115	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2294	0.12122172902134855	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2290	0.12101035721834706	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	2209	0.11673007820756712	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2203	0.11641302050306489	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2170	0.11466920312830269	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	2117	0.11186852673853308	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	2112	0.11160431198478124	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	2078	0.10980765165926865	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	2051	0.10838089198900867	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1906	0.10071866413020503	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.056859015007398E-4
2	0.0	0.0	0.0	0.0	1.056859015007398E-4
3	0.0	0.0	0.0	0.0	1.056859015007398E-4
4	0.0	0.0	0.0	5.28429507503699E-5	1.056859015007398E-4
5	0.0	0.0	0.0	5.28429507503699E-5	1.056859015007398E-4
6	0.0	0.0	0.0	5.28429507503699E-5	1.056859015007398E-4
7	0.0	0.0	0.0	1.056859015007398E-4	1.056859015007398E-4
8	0.0	0.0	0.0	1.056859015007398E-4	1.056859015007398E-4
9	0.0	0.0	0.0	1.585288522511097E-4	1.056859015007398E-4
10	0.0	0.0	0.0	1.585288522511097E-4	1.056859015007398E-4
11	0.0	0.0	0.0	1.585288522511097E-4	1.056859015007398E-4
12	0.0	0.0	0.0	1.585288522511097E-4	1.056859015007398E-4
13	0.0	0.0	0.0	1.585288522511097E-4	2.113718030014796E-4
14	0.0	0.0	0.0	2.113718030014796E-4	2.113718030014796E-4
15	0.0	0.0	0.0	2.113718030014796E-4	2.642147537518495E-4
16	0.0	0.0	0.0	2.642147537518495E-4	2.642147537518495E-4
17	0.0	0.0	0.0	2.642147537518495E-4	2.642147537518495E-4
18	5.28429507503699E-5	0.0	0.0	4.227436060029592E-4	2.642147537518495E-4
19	5.28429507503699E-5	0.0	0.0	4.755865567533291E-4	2.642147537518495E-4
20	5.28429507503699E-5	0.0	0.0	7.926442612555486E-4	4.755865567533291E-4
21	5.28429507503699E-5	0.0	0.0	0.0011625449165081378	4.755865567533291E-4
22	5.28429507503699E-5	0.0	0.0	0.0014796026210103573	4.755865567533291E-4
23	5.28429507503699E-5	0.0	0.0	0.001955189177763686	4.755865567533291E-4
24	5.28429507503699E-5	0.0	0.0	0.0023250898330162756	4.755865567533291E-4
25	5.28429507503699E-5	0.0	0.0	0.002747833439019235	4.755865567533291E-4
26	5.28429507503699E-5	0.0	0.0	0.003910378355527372	4.755865567533291E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGATCG	40	1.596853E-4	23.993856	5
TAACGCA	60	1.141145E-4	18.661396	4
GTCCTAT	800	0.0	18.017292	1
CTAGCGG	605	0.0	17.718685	29
TAGCGGC	615	0.0	17.690737	30
TCTAGCG	610	0.0	17.572521	28
CCTATTC	795	0.0	17.10254	3
GTATTAT	190	0.0	16.858286	1
CTATTCC	830	0.0	16.573631	4
ATCGTTT	525	0.0	16.152088	29
GCGCAAG	760	0.0	15.593914	1
TATTCCA	920	0.0	15.474299	5
CGGTCCA	725	0.0	15.4488125	10
AGCGGCG	710	0.0	15.323666	31
TCCTATT	920	0.0	15.307711	2
CGTTTAT	555	0.0	15.279002	31
CATCGTT	565	0.0	15.007784	28
CCGGTCC	750	0.0	14.933851	9
GTATTAG	505	0.0	14.905394	1
ATACCGT	570	0.0	14.873384	6
>>END_MODULE