FastQCFastQC Report
Thu 2 Feb 2017
SRR4062785_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062785_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1892400
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT51220.27066159374339466No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT37530.19831959416613823No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC32920.1739589938702177No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT31580.16687803846966814No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG30010.15858169520186008No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA28480.15049672373705347No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC28390.15002113718030016No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC27520.14542380046501796No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG26060.13770872965546396No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC25520.13485521031494396No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG24650.1302578735996618No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA24020.1269287677023885No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG24000.12682308180088775No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT23430.12381103360811667No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT21830.11535616148805748No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA21580.11403508771929824No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG21550.11387655886704715No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG21040.11118156837877827No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC20360.1075882477277531No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT20050.10595011625449166No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG19810.10468188543648277No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT19740.10431198478123019No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG19450.10277953920946946No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT19300.10198689494821392No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA18950.10013739167195096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG6550.019.05291629
TATAACG601.138453E-418.6671452
TAGCGGC6900.018.31834230
TCTAGCG6900.017.85458828
ATCGTTT6050.017.18961529
CGGTCCA7600.017.05261810
CGCCGGT7400.016.4337227
CATCGTT6050.016.39624828
ACCGTCG6850.016.3516468
AAGACGG7650.016.3150045
TCGTTTA6250.016.12756230
AGCGGCG7900.015.99956431
CCGGTCC8250.015.9034389
TACCGTC7400.015.7850237
GCCGGTC7850.015.69558
GGTCCAA8900.015.64043711
CGTTTAT6450.015.62748131
GCGCAAG7500.015.5749661
ATACCGT7550.015.4714126
CGCAAGA7350.015.4561792
Produced by FastQC (version 0.11.5)