FastQCFastQC Report
Thu 2 Feb 2017
SRR4062784_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062784_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences866237
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT32350.3734543779589189No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT21210.24485215939748592No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT12160.14037728704730923No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA12090.139569194112004No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA9840.11359477833433576No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9760.11267124355112978No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG9300.10736091854769537No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG9170.10586017452498565No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG9160.10574473267708491No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC9160.10574473267708491No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC9000.10389766311067294No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC8850.10216603539216172No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT8760.10112705876105499No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAG400.004462792520.015311
GTATAGC507.157028E-419.2146991
CGGGTCG1750.017.37168129
AACCGCG1750.016.456437
TCTAGCG2550.016.31396128
GTACTAA703.673613E-416.0122491
CTAGCGG2600.016.0002329
TAAGACT703.6979275E-415.99838354
CGACCCG1601.8189894E-1115.9983835
TAGTACT805.623151E-515.9983834
GACCCGG1850.015.5659956
CGCGGTC1652.910383E-1115.51448110
CATCGTT1451.2187229E-915.448528
TAAGAGT1153.3457945E-715.3028024
GACGAAA2000.015.19934222
GTATAGA951.4212479E-515.16951
AGAACCG1901.8189894E-1215.1563645
TGTGGCG859.4313895E-515.05817112
ATACCGT1603.0377123E-1014.9984846
GTCATAC756.2060106E-414.9447661
Produced by FastQC (version 0.11.5)