##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062784_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	866237
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.169125770430032	32.0	32.0	32.0	32.0	32.0
2	30.79248750630601	32.0	32.0	32.0	32.0	32.0
3	30.85389910613377	32.0	32.0	32.0	32.0	32.0
4	30.916018364489165	32.0	32.0	32.0	32.0	32.0
5	30.84121320146796	32.0	32.0	32.0	32.0	32.0
6	34.48143406481136	36.0	36.0	36.0	32.0	36.0
7	34.39082144955711	36.0	36.0	36.0	32.0	36.0
8	34.36670333869368	36.0	36.0	36.0	32.0	36.0
9	34.48882926958788	36.0	36.0	36.0	32.0	36.0
10	34.23499573442372	36.0	36.0	36.0	32.0	36.0
11	34.508681804171374	36.0	36.0	36.0	32.0	36.0
12	34.33590460809224	36.0	36.0	36.0	32.0	36.0
13	34.42320288789327	36.0	36.0	36.0	32.0	36.0
14	34.3084987134006	36.0	36.0	36.0	32.0	36.0
15	34.269107646059915	36.0	36.0	36.0	32.0	36.0
16	34.27865237804435	36.0	36.0	36.0	32.0	36.0
17	34.20637192823673	36.0	36.0	36.0	32.0	36.0
18	34.23412992056446	36.0	36.0	36.0	32.0	36.0
19	34.22781063381038	36.0	36.0	36.0	32.0	36.0
20	34.19134601731397	36.0	36.0	36.0	32.0	36.0
21	34.17122565764335	36.0	36.0	36.0	32.0	36.0
22	34.1463941161599	36.0	36.0	36.0	32.0	36.0
23	34.103636764534414	36.0	36.0	36.0	32.0	36.0
24	34.094467218555664	36.0	36.0	36.0	32.0	36.0
25	34.07490559742888	36.0	36.0	36.0	32.0	36.0
26	34.01814745849	36.0	36.0	36.0	32.0	36.0
27	34.02309529609102	36.0	36.0	36.0	32.0	36.0
28	34.00453801904098	36.0	36.0	36.0	32.0	36.0
29	33.97340219824367	36.0	36.0	36.0	32.0	36.0
30	33.93713267847021	36.0	36.0	36.0	32.0	36.0
31	33.967939490001	36.0	36.0	36.0	32.0	36.0
32	33.90227501249658	36.0	36.0	36.0	32.0	36.0
33	33.864817596108225	36.0	36.0	36.0	32.0	36.0
34	33.86741042001208	36.0	36.0	36.0	32.0	36.0
35	33.80252517498098	36.0	36.0	36.0	32.0	36.0
36	33.76560571760384	36.0	36.0	36.0	32.0	36.0
37	33.765673828294105	36.0	36.0	36.0	32.0	36.0
38	33.14283966166303	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	2.0
9	4.0
10	6.0
11	6.0
12	6.0
13	1.0
14	125.0
15	291.0
16	360.0
17	439.0
18	572.0
19	733.0
20	1014.0
21	1327.0
22	1968.0
23	2914.0
24	4133.0
25	6163.0
26	8929.0
27	12594.0
28	17396.0
29	23089.0
30	31507.0
31	42119.0
32	57275.0
33	85199.0
34	184522.0
35	383541.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.65684002473517	17.358076205116944	11.498384701184166	25.486699068963723
2	16.28860858918382	20.40023563656525	38.193221983505445	25.11793379074549
3	18.771705397716776	25.05622621642591	28.705619388923783	27.466448996933536
4	12.263727281857927	15.91052085341596	36.65403313041942	35.17171873430669
5	14.128119671637208	37.0544088973341	33.842239479495795	14.975231951532894
6	32.91689418435917	36.18772316455273	17.565712689311074	13.329669961777027
7	29.264426784386703	30.99894255145249	21.632211083353727	18.104419580807075
8	27.651188939709133	33.091173978393826	20.091222464137864	19.166414617759173
9	27.75289355993877	14.480267456599488	18.781645606152612	38.98519337730914
10	15.585219185182705	26.896751559341563	32.10783266819128	25.410196587284457
11	36.707753084367425	21.71507798800078	22.57432554896372	19.00284337866808
12	24.650346622951577	24.23878598269483	29.178090889675197	21.9327765046784
13	29.35714673320071	19.89220930514488	25.48067653208609	25.269967429568318
14	23.651804867010405	20.110534105786705	25.858099792416628	30.379561234786262
15	24.964551127204874	27.22381055752879	23.31993127306486	24.49170704220148
16	25.150783241585696	26.12948886097462	24.40402519673403	24.315702700705657
17	23.552335315512963	26.099994226661277	25.822873968015703	24.524796489810058
18	24.37014759955899	24.99518006915302	27.096439023545503	23.53823330774248
19	25.002770799468006	25.49401045514999	26.28518915324368	23.21802959213832
20	25.428743940438213	24.33360694441077	26.073057621107537	24.164591494043474
21	26.595642742271586	24.574370199506575	25.09204132594925	23.737945732272593
22	25.278782512119346	24.751523072074182	25.594884186488443	24.374810229318026
23	23.91425572961946	24.61343613224854	26.006486122030992	25.465822016101008
24	24.069874844132453	25.651988177157897	25.94917563386136	24.328961344848288
25	24.42881884308971	24.861909710195125	25.95600969864912	24.753261748066045
26	24.133587200125625	25.607022980794873	26.397142959410946	23.86224685966855
27	24.80927487508197	25.34519862197634	25.49528497935735	24.35024152358434
28	23.96492228619323	25.106125554294607	26.41553652716318	24.513415632348988
29	23.802074253327685	25.637221567339342	26.32359783546135	24.237106343871623
30	24.00241049256938	25.556416262512798	26.530772454350142	23.910400790567678
31	24.49627044444111	25.37321531444061	25.352896423297917	24.77761781782036
32	23.934258544114915	25.688164482942945	25.396654971651422	24.980922001290722
33	23.679823406898397	25.067423098414633	26.05879663623391	25.193956858453053
34	24.429250630958897	25.437630319277904	26.167071911995954	23.966047137767248
35	25.24255030256007	25.122819414184587	25.971442393509363	23.66318788974598
36	23.996919549201195	25.987897569237983	25.550538212945977	24.46464466861485
37	24.854790611005086	25.85183158366266	25.41369234197258	23.879685463359667
38	23.822778321665407	25.790817740177697	25.931317767885183	24.455086170271716
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	487.0
1	390.0
2	293.0
3	293.0
4	686.5
5	1080.0
6	1080.0
7	1235.0
8	1390.0
9	1294.5
10	1199.0
11	1199.0
12	1509.0
13	1819.0
14	2294.0
15	2769.0
16	2769.0
17	3911.5
18	5054.0
19	5054.0
20	6174.0
21	7294.0
22	7522.0
23	7750.0
24	7750.0
25	8983.0
26	10216.0
27	10216.0
28	13146.0
29	16076.0
30	19246.5
31	22417.0
32	22417.0
33	27683.5
34	32950.0
35	32950.0
36	36464.5
37	39979.0
38	44921.5
39	49864.0
40	49864.0
41	53445.5
42	57027.0
43	63712.5
44	70398.0
45	70398.0
46	72930.0
47	75462.0
48	75462.0
49	78167.5
50	80873.0
51	79642.5
52	78412.0
53	78412.0
54	73990.5
55	69569.0
56	69569.0
57	67079.0
58	64589.0
59	57277.0
60	49965.0
61	49965.0
62	46776.0
63	43587.0
64	35786.5
65	27986.0
66	27986.0
67	23192.0
68	18398.0
69	18398.0
70	14838.0
71	11278.0
72	8739.0
73	6200.0
74	6200.0
75	4755.0
76	3310.0
77	3310.0
78	3164.5
79	3019.0
80	2367.0
81	1715.0
82	1715.0
83	1777.5
84	1840.0
85	1840.0
86	1225.5
87	611.0
88	535.0
89	459.0
90	459.0
91	337.0
92	215.0
93	189.0
94	163.0
95	163.0
96	144.5
97	126.0
98	126.0
99	262.0
100	398.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12375366094960155
2	0.05737459840667162
3	0.010736091854769538
4	0.002770604349617945
5	0.0
6	4.6176739160299084E-4
7	1.1544184790074771E-4
8	3.4632554370224314E-4
9	3.4632554370224314E-4
10	6.926510874044863E-4
11	0.001616185870610468
12	0.00253972065381645
13	0.01316037066068524
14	0.00911990598415907
15	0.02320381142805029
16	0.010736091854769538
17	0.021587625557439823
18	0.004848557611831404
19	0.0070419527219456116
20	0.0050794413076329
21	0.005772092395037386
22	0.006926510874044864
23	0.009235347832059818
24	0.013506696204387483
25	0.01581553316240244
26	0.01916334675152412
27	0.007965487505151593
28	0.0053103250034343946
29	0.010851533702670285
30	0.002770604349617945
31	0.00554120869923589
32	0.006464743482441872
33	0.007503720113548601
34	0.010966975550571034
35	0.014776556531295707
36	0.015007440227097203
37	0.008542696744655331
38	0.0051948831555336476
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	866237.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.95974761923726
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.84678810609071	52.789726178431366
2	10.783925209606581	13.579064190831211
3	2.62039922986054	4.949390225209899
4	0.9558640744705336	2.407238435478424
5	0.47642436178450254	1.4997778788804235
6	0.2828313930440807	1.0684195874911568
7	0.1730568102986556	0.7626929170135502
8	0.13082540117552843	0.6589387392157389
9	0.08107203300895197	0.4593847263499879
>10	0.5044061375139767	6.143478042678537
>50	0.06493884655314477	2.9093184055408026
>100	0.07266207562335614	9.150150948096348
>500	0.006070502486404603	2.7212448077978206
>1k	7.35818483200558E-4	0.9011749169847992
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3235	0.3734543779589189	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2121	0.24485215939748592	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1216	0.14037728704730923	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1209	0.139569194112004	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	984	0.11359477833433576	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	976	0.11267124355112978	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	930	0.10736091854769537	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	917	0.10586017452498565	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	916	0.10574473267708491	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	916	0.10574473267708491	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	900	0.10389766311067294	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	885	0.10216603539216172	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	876	0.10112705876105499	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	1.1544184790074772E-4	0.0	0.0	0.0
8	0.0	1.1544184790074772E-4	0.0	0.0	0.0
9	0.0	1.1544184790074772E-4	0.0	0.0	0.0
10	0.0	1.1544184790074772E-4	0.0	0.0	0.0
11	0.0	1.1544184790074772E-4	0.0	0.0	0.0
12	0.0	1.1544184790074772E-4	0.0	0.0	1.1544184790074772E-4
13	0.0	1.1544184790074772E-4	0.0	0.0	2.3088369580149545E-4
14	0.0	1.1544184790074772E-4	0.0	0.0	2.3088369580149545E-4
15	0.0	1.1544184790074772E-4	0.0	1.1544184790074772E-4	2.3088369580149545E-4
16	0.0	1.1544184790074772E-4	0.0	2.3088369580149545E-4	2.3088369580149545E-4
17	0.0	1.1544184790074772E-4	0.0	2.3088369580149545E-4	2.3088369580149545E-4
18	0.0	1.1544184790074772E-4	0.0	3.4632554370224314E-4	2.3088369580149545E-4
19	0.0	1.1544184790074772E-4	0.0	3.4632554370224314E-4	2.3088369580149545E-4
20	0.0	1.1544184790074772E-4	0.0	4.617673916029909E-4	2.3088369580149545E-4
21	0.0	1.1544184790074772E-4	0.0	5.772092395037385E-4	2.3088369580149545E-4
22	0.0	1.1544184790074772E-4	0.0	0.0010389766311067294	2.3088369580149545E-4
23	0.0	1.1544184790074772E-4	0.0	0.0018470695664119636	2.3088369580149545E-4
24	0.0	1.1544184790074772E-4	0.0	0.003347813589121684	2.3088369580149545E-4
25	0.0	2.3088369580149545E-4	0.0	0.0038095809807246746	2.3088369580149545E-4
26	0.0	2.3088369580149545E-4	0.0	0.004617673916029908	2.3088369580149545E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGATTAG	40	0.0044627925	20.01531	1
GTATAGC	50	7.157028E-4	19.214699	1
CGGGTCG	175	0.0	17.371681	29
AACCGCG	175	0.0	16.45643	7
TCTAGCG	255	0.0	16.313961	28
GTACTAA	70	3.673613E-4	16.012249	1
CTAGCGG	260	0.0	16.00023	29
TAAGACT	70	3.6979275E-4	15.9983835	4
CGACCCG	160	1.8189894E-11	15.998383	5
TAGTACT	80	5.623151E-5	15.998383	4
GACCCGG	185	0.0	15.565995	6
CGCGGTC	165	2.910383E-11	15.514481	10
CATCGTT	145	1.2187229E-9	15.4485	28
TAAGAGT	115	3.3457945E-7	15.302802	4
GACGAAA	200	0.0	15.199342	22
GTATAGA	95	1.4212479E-5	15.1695	1
AGAACCG	190	1.8189894E-12	15.156364	5
TGTGGCG	85	9.4313895E-5	15.058171	12
ATACCGT	160	3.0377123E-10	14.998484	6
GTCATAC	75	6.2060106E-4	14.944766	1
>>END_MODULE