FastQCFastQC Report
Thu 2 Feb 2017
SRR4062784_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062784_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences866237
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT37500.4329069296278039No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT27030.3120393148757211No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT16780.19371142077745468No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA11810.13633682237078304No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC11440.1320654739984554No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11270.1301029625841427No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG10240.11821245225036565No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA9420.10874622072250435No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC9340.10782268593929836No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC9130.10539840713338267No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG8770.10124250060895575No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC8690.10031896582574976No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA8690.10031896582574976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTAT2000.021.59937731
TAGGACG852.3921348E-718.8251574
GCATCGT2300.018.78206627
TCTAGCG2250.018.48835628
TCGTTTA2350.018.38244830
ATCGTTT2400.017.99948129
AATAGCC450.00884414917.7793183
CATCGTT2450.017.63214328
CCGTCGT2000.017.6005089
TAGCGGC2400.017.33283430
CTAGCGG2400.017.33283429
CGTCGTA1950.017.23027210
ATACCGT2150.017.1177626
ACCGTCG2000.016.8014558
TAAGAGT1354.4201443E-1016.594034
GTATATA1601.8189894E-1116.0013871
TACCGTC2200.016.0013877
AGCGTAT1800.016.0013858
TAGCGTA1800.016.0013857
ACCGAGT600.002439843516.0013858
Produced by FastQC (version 0.11.5)