##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062784_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	866237
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.113346578361348	32.0	32.0	32.0	32.0	32.0
2	31.231405492954007	32.0	32.0	32.0	32.0	32.0
3	31.334892183086154	32.0	32.0	32.0	32.0	32.0
4	31.443477939640076	32.0	32.0	32.0	32.0	32.0
5	31.349892696802375	32.0	32.0	32.0	32.0	32.0
6	34.855114708792165	36.0	36.0	36.0	32.0	36.0
7	34.88318901178315	36.0	36.0	36.0	36.0	36.0
8	34.823128081575824	36.0	36.0	36.0	32.0	36.0
9	34.93913444011281	36.0	36.0	36.0	32.0	36.0
10	34.77619058063786	36.0	36.0	36.0	32.0	36.0
11	34.97855667675243	36.0	36.0	36.0	36.0	36.0
12	34.86225132382939	36.0	36.0	36.0	32.0	36.0
13	34.91606454122832	36.0	36.0	36.0	32.0	36.0
14	34.86537633465206	36.0	36.0	36.0	32.0	36.0
15	34.83047826403167	36.0	36.0	36.0	32.0	36.0
16	34.83910638774377	36.0	36.0	36.0	32.0	36.0
17	34.80823146552272	36.0	36.0	36.0	32.0	36.0
18	34.80886755010465	36.0	36.0	36.0	32.0	36.0
19	34.79593229104737	36.0	36.0	36.0	32.0	36.0
20	34.78870794020574	36.0	36.0	36.0	32.0	36.0
21	34.77181187134698	36.0	36.0	36.0	32.0	36.0
22	34.75784571658795	36.0	36.0	36.0	32.0	36.0
23	34.69110070338718	36.0	36.0	36.0	32.0	36.0
24	34.68472254129066	36.0	36.0	36.0	32.0	36.0
25	34.65815475441479	36.0	36.0	36.0	32.0	36.0
26	34.598636400892595	36.0	36.0	36.0	32.0	36.0
27	34.58222287895807	36.0	36.0	36.0	32.0	36.0
28	34.54550544481476	36.0	36.0	36.0	32.0	36.0
29	34.496851323598506	36.0	36.0	36.0	32.0	36.0
30	34.48120433553404	36.0	36.0	36.0	32.0	36.0
31	34.47041860368467	36.0	36.0	36.0	32.0	36.0
32	34.43865824249022	36.0	36.0	36.0	32.0	36.0
33	34.383186125736955	36.0	36.0	36.0	32.0	36.0
34	34.37804550024993	36.0	36.0	36.0	32.0	36.0
35	34.336404471293655	36.0	36.0	36.0	32.0	36.0
36	34.303950304593315	36.0	36.0	36.0	32.0	36.0
37	34.28620227489706	36.0	36.0	36.0	32.0	36.0
38	33.85212361051306	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	1.0
21	25.0
22	51.0
23	249.0
24	589.0
25	1569.0
26	3189.0
27	6254.0
28	10664.0
29	16826.0
30	25949.0
31	37110.0
32	52998.0
33	84178.0
34	200361.0
35	426223.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.900932395549994	18.618816933670146	12.1819856931084	26.298264977671458
2	15.359999630586088	21.3215320980286	37.37256663014856	25.945901641236752
3	17.671260867406957	25.78486026341521	29.04840130356935	27.49547756560849
4	11.86188075549763	16.795749892927684	36.622656386185305	34.719712965389384
5	13.629557798980645	37.64486542335509	33.4435805526214	15.281996225042857
6	32.94033034877331	36.65802391034314	17.111041332846938	13.290604408036607
7	28.96675367194201	31.30769967826134	21.570458688271373	18.155087961525275
8	27.56520002447418	33.336142476671604	19.847405794608523	19.251251704245693
9	27.626016222618848	14.170547611516074	18.632806817840724	39.570629348024354
10	15.417996185761718	27.138928780875244	32.1396907049876	25.30338432837544
11	36.832948309879804	21.58405691189255	22.562605275340836	19.02038950288681
12	24.46535994190966	24.32752237551617	29.210135332478295	21.996982350095877
13	29.612830683555057	19.798184328520172	25.457612013354332	25.131372974570443
14	23.515300680184154	19.991318763016082	25.81063359176945	30.682746965030315
15	24.941211238956544	27.25824456817245	23.22228212371441	24.578262069156594
16	25.10687029069412	26.052800792392844	24.395748507625513	24.44458040928753
17	23.497841814653494	26.17782431366935	25.79294119276826	24.53139267890889
18	24.399788972302037	24.9031154291493	27.12975779145892	23.567337807089743
19	25.03760518195367	25.429183930033005	26.297768393638233	23.235442494375096
20	25.396629328925	24.243711593940226	26.074619301646084	24.285039775488695
21	26.534108487756225	24.535692351737865	25.251894402910985	23.67830475759493
22	25.18410088693972	24.645218340939028	25.804254493862533	24.36642627825872
23	23.752764835449	24.429716613024627	26.271477980596515	25.54604057092986
24	24.083247425358188	25.474090808866396	25.99057763637434	24.452084129401076
25	24.371880180458906	24.688537534805757	26.20694591312298	24.732636371612355
26	24.096869563410475	25.463354717011626	26.53846464651129	23.90131107306661
27	24.90198975798743	25.05622024482935	25.666908325213917	24.374881671969302
28	23.9462179519	24.92689644981685	26.530845484549843	24.59604011373331
29	23.897732375781686	25.26467929677444	26.548969854670258	24.288618472773617
30	23.96018641549599	25.35368496150592	26.676302212904783	24.009826410093314
31	24.351880605423226	25.196914932056703	25.52569331487803	24.925511147642045
32	23.98673804051316	25.417870628938733	25.612967351890997	24.982423978657113
33	23.854095357275202	24.78952065081496	26.22019147184893	25.136192520060906
34	24.433036224497453	25.158934564097358	26.39681749913707	24.01121171226812
35	25.248055670676735	24.91408240469987	26.074157534254482	23.76370439036892
36	24.1622096493223	25.705782597603193	25.774701380799943	24.35730637227456
37	24.98692621072524	25.526732291509134	25.652794789416756	23.83354670834887
38	24.046075150334147	25.223351115225974	26.14180645712432	24.58876727731556
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	280.0
1	223.5
2	167.0
3	167.0
4	547.0
5	927.0
6	927.0
7	1125.5
8	1324.0
9	1317.5
10	1311.0
11	1311.0
12	1617.5
13	1924.0
14	2380.5
15	2837.0
16	2837.0
17	4266.0
18	5695.0
19	5695.0
20	6573.0
21	7451.0
22	7709.0
23	7967.0
24	7967.0
25	8979.5
26	9992.0
27	9992.0
28	12858.5
29	15725.0
30	18938.5
31	22152.0
32	22152.0
33	27505.5
34	32859.0
35	32859.0
36	36266.5
37	39674.0
38	44899.5
39	50125.0
40	50125.0
41	53659.5
42	57194.0
43	63617.0
44	70040.0
45	70040.0
46	73184.0
47	76328.0
48	76328.0
49	78992.5
50	81657.0
51	79791.0
52	77925.0
53	77925.0
54	74082.0
55	70239.0
56	70239.0
57	67350.5
58	64462.0
59	57164.5
60	49867.0
61	49867.0
62	46754.0
63	43641.0
64	35933.0
65	28225.0
66	28225.0
67	23255.5
68	18286.0
69	18286.0
70	14806.0
71	11326.0
72	8957.0
73	6588.0
74	6588.0
75	4828.5
76	3069.0
77	3069.0
78	3035.5
79	3002.0
80	2315.5
81	1629.0
82	1629.0
83	1595.5
84	1562.0
85	1562.0
86	991.5
87	421.0
88	350.5
89	280.0
90	280.0
91	166.5
92	53.0
93	36.5
94	20.0
95	20.0
96	11.5
97	3.0
98	3.0
99	6.5
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009350789679960565
2	0.0
3	0.0
4	0.0
5	2.3088369580149542E-4
6	1.1544184790074771E-4
7	6.926510874044863E-4
8	0.002077953262213459
9	0.004502232068129161
10	0.0010389766311067294
11	0.006464743482441872
12	0.0
13	1.1544184790074771E-4
14	1.1544184790074771E-4
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.1544184790074771E-4
22	0.0
23	1.1544184790074771E-4
24	0.0
25	1.1544184790074771E-4
26	0.0
27	1.1544184790074771E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	866237.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.057475824365646
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.65461567679951	50.46682198458421
2	11.552057774763243	14.10678976608564
3	2.84786974019359	5.216512134384379
4	1.0364400171043482	2.5312964515101544
5	0.5053369114351937	1.5427298126556972
6	0.30164398448075264	1.1050572173999331
7	0.1946395976924367	0.8318941771399148
8	0.12469560962058981	0.6090879335850959
9	0.09299643992329175	0.5110315094131613
>10	0.5324318702504566	6.190982775373527
>50	0.07264736711591704	3.1556797442279856
>100	0.07682809955077541	9.559511198930466
>500	0.0064657311311784324	2.7087797451103244
>1k	0.0013311799387720302	1.4638255495994625
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3750	0.4329069296278039	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2703	0.3120393148757211	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1678	0.19371142077745468	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1181	0.13633682237078304	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1144	0.1320654739984554	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1127	0.1301029625841427	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1024	0.11821245225036565	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	942	0.10874622072250435	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	934	0.10782268593929836	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	913	0.10539840713338267	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	877	0.10124250060895575	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	869	0.10031896582574976	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	869	0.10031896582574976	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1544184790074772E-4
2	0.0	0.0	0.0	0.0	1.1544184790074772E-4
3	0.0	0.0	0.0	0.0	1.1544184790074772E-4
4	0.0	0.0	0.0	0.0	1.1544184790074772E-4
5	0.0	0.0	0.0	0.0	1.1544184790074772E-4
6	0.0	0.0	0.0	0.0	1.1544184790074772E-4
7	0.0	0.0	0.0	0.0	1.1544184790074772E-4
8	0.0	0.0	0.0	0.0	1.1544184790074772E-4
9	0.0	0.0	0.0	1.1544184790074772E-4	1.1544184790074772E-4
10	0.0	0.0	0.0	1.1544184790074772E-4	1.1544184790074772E-4
11	0.0	0.0	0.0	1.1544184790074772E-4	1.1544184790074772E-4
12	0.0	0.0	0.0	1.1544184790074772E-4	2.3088369580149545E-4
13	0.0	0.0	0.0	1.1544184790074772E-4	2.3088369580149545E-4
14	0.0	0.0	0.0	1.1544184790074772E-4	2.3088369580149545E-4
15	0.0	0.0	0.0	3.4632554370224314E-4	2.3088369580149545E-4
16	0.0	0.0	0.0	4.617673916029909E-4	2.3088369580149545E-4
17	0.0	0.0	0.0	4.617673916029909E-4	2.3088369580149545E-4
18	0.0	0.0	0.0	5.772092395037385E-4	2.3088369580149545E-4
19	0.0	0.0	0.0	5.772092395037385E-4	2.3088369580149545E-4
20	0.0	0.0	0.0	6.926510874044863E-4	2.3088369580149545E-4
21	0.0	0.0	0.0	8.08092935305234E-4	2.3088369580149545E-4
22	0.0	0.0	0.0	0.001154418479007477	2.3088369580149545E-4
23	0.0	0.0	0.0	0.0018470695664119636	2.3088369580149545E-4
24	0.0	0.0	0.0	0.003347813589121684	2.3088369580149545E-4
25	0.0	1.1544184790074772E-4	0.0	0.0038095809807246746	2.3088369580149545E-4
26	0.0	1.1544184790074772E-4	0.0	0.004617673916029908	3.4632554370224314E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTAT	200	0.0	21.599377	31
TAGGACG	85	2.3921348E-7	18.825157	4
GCATCGT	230	0.0	18.782066	27
TCTAGCG	225	0.0	18.488356	28
TCGTTTA	235	0.0	18.382448	30
ATCGTTT	240	0.0	17.999481	29
AATAGCC	45	0.008844149	17.779318	3
CATCGTT	245	0.0	17.632143	28
CCGTCGT	200	0.0	17.600508	9
TAGCGGC	240	0.0	17.332834	30
CTAGCGG	240	0.0	17.332834	29
CGTCGTA	195	0.0	17.230272	10
ATACCGT	215	0.0	17.117762	6
ACCGTCG	200	0.0	16.801455	8
TAAGAGT	135	4.4201443E-10	16.59403	4
GTATATA	160	1.8189894E-11	16.001387	1
TACCGTC	220	0.0	16.001387	7
AGCGTAT	180	0.0	16.001385	8
TAGCGTA	180	0.0	16.001385	7
ACCGAGT	60	0.0024398435	16.001385	8
>>END_MODULE