FastQCFastQC Report
Thu 2 Feb 2017
SRR4062783_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062783_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4867380
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT119730.24598449268394906No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT73760.15153943189148988No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA60940.1252008267281371No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC60360.12400922056630057No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC59870.12300251880888692No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG58390.11996186860282122No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT56840.11677740385998216No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG56790.11667467919085832No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG56760.11661304438938401No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA54050.11104536732287186No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC51480.10576531932990645No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG51220.10523115105046246No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT49910.10253976471941784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG12950.017.66774429
GTATCAA84450.017.2198581
TCTAGCG13500.017.06558828
TAGCGGC13750.016.87252630
ATCGTTT9900.014.8685129
AGCGGCG15950.014.64559331
AAGACGG14300.014.2067025
CATCGTT10550.013.80007628
CTTTCGT15550.013.78648427
TTCGTTC15450.013.77329929
GTATTAG13950.013.6620611
CGGTCCA17800.013.57398810
AACGCAG106350.013.41692356
TAGGACG7650.013.3829864
GTCCTAT17250.013.2767071
CAAGACG15600.013.2281664
CGCCGGT17950.013.1905537
TTTCGTT16450.013.129868528
TACCGTC10850.013.1227867
TCCTATA3550.013.0737592
Produced by FastQC (version 0.11.5)