##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062783_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4867380
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.255099868923324	32.0	32.0	32.0	32.0	32.0
2	30.875122550530264	32.0	32.0	32.0	32.0	32.0
3	30.925506329894112	32.0	32.0	32.0	32.0	32.0
4	30.990876611236434	32.0	32.0	32.0	32.0	32.0
5	30.909291240872914	32.0	32.0	32.0	32.0	32.0
6	34.56474345541133	36.0	36.0	36.0	32.0	36.0
7	34.5006494253582	36.0	36.0	36.0	32.0	36.0
8	34.489880592844614	36.0	36.0	36.0	32.0	36.0
9	34.6238728022057	36.0	36.0	36.0	32.0	36.0
10	34.34493156482543	36.0	36.0	36.0	32.0	36.0
11	34.60193204557688	36.0	36.0	36.0	32.0	36.0
12	34.42096795401222	36.0	36.0	36.0	32.0	36.0
13	34.50632105978987	36.0	36.0	36.0	32.0	36.0
14	34.41010523115105	36.0	36.0	36.0	32.0	36.0
15	34.35613636083478	36.0	36.0	36.0	32.0	36.0
16	34.372375692877895	36.0	36.0	36.0	32.0	36.0
17	34.30195259051071	36.0	36.0	36.0	32.0	36.0
18	34.32867374234188	36.0	36.0	36.0	32.0	36.0
19	34.319584869067135	36.0	36.0	36.0	32.0	36.0
20	34.306791538774455	36.0	36.0	36.0	32.0	36.0
21	34.2946794784874	36.0	36.0	36.0	32.0	36.0
22	34.265836445890805	36.0	36.0	36.0	32.0	36.0
23	34.21618139532973	36.0	36.0	36.0	32.0	36.0
24	34.19934831469908	36.0	36.0	36.0	32.0	36.0
25	34.17586052455325	36.0	36.0	36.0	32.0	36.0
26	34.131417107355496	36.0	36.0	36.0	32.0	36.0
27	34.13547041734979	36.0	36.0	36.0	32.0	36.0
28	34.124400807005	36.0	36.0	36.0	32.0	36.0
29	34.08724837592298	36.0	36.0	36.0	32.0	36.0
30	34.06367224256171	36.0	36.0	36.0	32.0	36.0
31	34.08246551532857	36.0	36.0	36.0	32.0	36.0
32	34.039011542143825	36.0	36.0	36.0	32.0	36.0
33	34.005468650485476	36.0	36.0	36.0	32.0	36.0
34	34.01143222842679	36.0	36.0	36.0	32.0	36.0
35	33.97899979044168	36.0	36.0	36.0	32.0	36.0
36	33.93965500947122	36.0	36.0	36.0	32.0	36.0
37	33.93559307060472	36.0	36.0	36.0	32.0	36.0
38	33.35112524602558	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	10.0
8	4.0
9	19.0
10	41.0
11	50.0
12	26.0
13	25.0
14	507.0
15	1242.0
16	1714.0
17	2033.0
18	2538.0
19	3100.0
20	4313.0
21	6151.0
22	9088.0
23	14293.0
24	20868.0
25	31644.0
26	46409.0
27	66160.0
28	92656.0
29	123846.0
30	166043.0
31	223840.0
32	304623.0
33	456029.0
34	1021594.0
35	2268514.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.49633416697899	17.91171145300777	11.968005779431264	25.623948600581976
2	16.394465712053318	19.781973376923307	38.128121064141936	25.695439846881442
3	18.39054787345851	24.19062303579827	28.897659426658056	28.52116966408516
4	12.463886088989279	15.559255577804084	36.68608356525816	35.29077476794848
5	14.557219357907206	36.46328146748806	33.72508533878187	15.254413835822872
6	33.74037825037731	36.02336216074511	16.954927087754797	13.281332501122787
7	29.86349945966824	30.77049665323028	21.291351815555803	18.074652071545678
8	27.69388459920939	33.16119372136927	19.782338171746655	19.36258350767468
9	27.338151815045908	14.570928448011966	19.201190786810947	38.88972895013118
10	16.03280136106665	26.379993750204168	31.48477439202656	26.102430496702627
11	36.983254754389094	21.499922338746064	22.82199218785312	18.69483071901172
12	24.866130579467676	23.773643518517567	28.761046037767173	22.599179864247585
13	28.79090278264368	19.615808143251463	25.804730212748822	25.788558861356037
14	23.503485169639532	20.219819236361154	25.239371691431188	31.03732390256812
15	24.812265087327408	27.54020102051511	22.51974345327023	25.127790438887253
16	25.563756210420767	26.049864182361375	23.966327088324615	24.42005251889324
17	23.72448670303216	26.21077258347401	25.48741506698573	24.5773256465081
18	24.751397289106066	24.90185640205272	26.61349061238277	23.733255696458446
19	25.39944100962628	25.086516530035308	25.793955975655585	23.720086484682827
20	25.502442844988288	24.57658826016715	25.231376324693024	24.689592570151532
21	26.715440826881455	24.384669342341926	24.804265739179606	24.095624091597017
22	25.567305671238344	24.449737692462108	25.358121239852828	24.62483539644672
23	23.84027582295881	24.276529775688378	26.142135943076582	25.74105845827623
24	24.55303002594388	25.24740797483612	25.587641457443695	24.611920541776307
25	24.671711334062806	24.65648486342924	25.694371240258707	24.977432562249245
26	24.30921413591199	25.653612701262162	26.142781933472985	23.894391229352866
27	25.024327480766082	25.04464832407492	25.115884283378985	24.815139911780008
28	24.09187775165212	25.03747629993861	26.04152883579381	24.82911711261546
29	24.267936488367674	25.071458291596677	25.90625304998639	24.754352170049263
30	24.08337550308358	25.26807325640667	26.27698324694955	24.371567993560202
31	25.026905238087217	24.949137902340663	24.9793408042026	25.044616055369524
32	24.424823526801163	25.14168811028003	24.879967816088175	25.553520546830633
33	24.023761111695553	24.794059680982564	25.7536967756358	25.428482431686085
34	24.995181569554916	24.812615452264662	25.814088720763223	24.378114257417195
35	25.61669318436571	24.7240916121261	25.73137375653978	23.927841446968404
36	24.313047802318046	25.395588916708878	25.29138896623005	24.999974314743028
37	25.358597736452598	25.085362801666943	25.203796112247822	24.352243349632637
38	24.284210301388597	25.084999769882177	25.721871383862823	24.908918544866403
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	506.0
1	555.0
2	604.0
3	604.0
4	1563.5
5	2523.0
6	2523.0
7	2952.0
8	3381.0
9	3284.0
10	3187.0
11	3187.0
12	4421.0
13	5655.0
14	8230.5
15	10806.0
16	10806.0
17	16493.5
18	22181.0
19	22181.0
20	28083.0
21	33985.0
22	38419.5
23	42854.0
24	42854.0
25	50623.5
26	58393.0
27	58393.0
28	73205.0
29	88017.0
30	104634.5
31	121252.0
32	121252.0
33	154098.0
34	186944.0
35	186944.0
36	207862.5
37	228781.0
38	255114.0
39	281447.0
40	281447.0
41	296758.0
42	312069.0
43	349919.0
44	387769.0
45	387769.0
46	390341.0
47	392913.0
48	392913.0
49	420711.5
50	448510.0
51	451296.5
52	454083.0
53	454083.0
54	429737.5
55	405392.0
56	405392.0
57	388995.5
58	372599.0
59	333422.0
60	294245.0
61	294245.0
62	276671.5
63	259098.0
64	213454.0
65	167810.0
66	167810.0
67	139326.0
68	110842.0
69	110842.0
70	88698.0
71	66554.0
72	51498.5
73	36443.0
74	36443.0
75	27236.0
76	18029.0
77	18029.0
78	17929.5
79	17830.0
80	13987.0
81	10144.0
82	10144.0
83	10470.0
84	10796.0
85	10796.0
86	7127.0
87	3458.0
88	3051.0
89	2644.0
90	2644.0
91	1972.5
92	1301.0
93	1138.0
94	975.0
95	975.0
96	914.5
97	854.0
98	854.0
99	1680.0
100	2506.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12331069281625845
2	0.054505709437109896
3	0.011936606552190294
4	0.0029173806031170773
5	3.0817400737152226E-4
6	4.5198854414489934E-4
7	0.0
8	2.67084139721986E-4
9	2.054493382476815E-4
10	4.314436103201312E-4
11	0.0018079541765795974
12	0.0028146559339932366
13	0.015388155434751345
14	0.009697208765290566
15	0.02485936992796946
16	0.011505162941870165
17	0.02146945584688272
18	0.005690946669460778
19	0.008033069125484347
20	0.0063483845518533585
21	0.006615468691575345
22	0.007868709654886202
23	0.010067017574136394
24	0.014833442221482605
25	0.017565918420176768
26	0.019846406074726035
27	0.009368489824094276
28	0.006245659882729518
29	0.011402438272746325
30	0.003020105272240918
31	0.0061223902797809095
32	0.0072318167063183895
33	0.008957591147598913
34	0.013354206986099296
35	0.016826300802485115
36	0.015922323714195316
37	0.010395736515332684
38	0.00632783961802859
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	4867380.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.82672858052374
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.57675199188836	32.684550679655246
2	13.773092900247526	12.072592085069738
3	4.707252970798916	6.189104949332044
4	2.2570884748766096	3.956832158825818
5	1.2047736372147135	2.6400643599589815
6	0.7182894632243116	1.8888166408189195
7	0.5000018652819962	1.5339412227628753
8	0.3472408544974099	1.2174744545701695
9	0.2615837544458414	1.0317924186455039
>10	1.4602143540153476	11.511167607107849
>50	0.09378208774158618	2.8427910460475476
>100	0.07416329573861836	6.9468323018415035
>500	0.013706446155994758	4.31040479706812
>1k	0.011492689628750304	9.604381949678267
>5k	5.18113057033816E-4	1.3220957745384616
>10k+	4.710118700307418E-5	0.2471575540790838
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	11973	0.24598449268394906	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	7376	0.15153943189148988	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	6094	0.1252008267281371	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	6036	0.12400922056630057	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	5987	0.12300251880888692	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	5839	0.11996186860282122	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	5684	0.11677740385998216	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	5679	0.11667467919085832	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	5676	0.11661304438938401	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	5405	0.11104536732287186	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	5148	0.10576531932990645	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	5122	0.10523115105046246	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	4991	0.10253976471941784	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.163480147430444E-5
2	0.0	0.0	0.0	0.0	8.21797352990726E-5
3	0.0	0.0	0.0	0.0	8.21797352990726E-5
4	0.0	0.0	0.0	0.0	8.21797352990726E-5
5	0.0	0.0	0.0	0.0	1.0272466912384075E-4
6	0.0	0.0	0.0	0.0	1.232696029486089E-4
7	0.0	0.0	0.0	0.0	1.232696029486089E-4
8	0.0	0.0	0.0	0.0	1.232696029486089E-4
9	0.0	0.0	0.0	0.0	1.4381453677337705E-4
10	0.0	0.0	0.0	0.0	1.4381453677337705E-4
11	0.0	2.054493382476815E-5	0.0	0.0	1.8490440442291335E-4
12	0.0	2.054493382476815E-5	0.0	0.0	3.4926387502105853E-4
13	0.0	2.054493382476815E-5	0.0	0.0	4.10898676495363E-4
14	0.0	2.054493382476815E-5	0.0	0.0	4.314436103201312E-4
15	0.0	2.054493382476815E-5	0.0	2.054493382476815E-5	5.752581470935082E-4
16	0.0	2.054493382476815E-5	0.0	2.054493382476815E-5	6.163480147430445E-4
17	0.0	2.054493382476815E-5	0.0	4.10898676495363E-5	6.163480147430445E-4
18	0.0	2.054493382476815E-5	0.0	6.163480147430444E-5	7.190726838668853E-4
19	0.0	2.054493382476815E-5	0.0	6.163480147430444E-5	7.190726838668853E-4
20	0.0	2.054493382476815E-5	0.0	1.0272466912384075E-4	8.21797352990726E-4
21	0.0	2.054493382476815E-5	0.0	1.8490440442291335E-4	8.628872206402624E-4
22	0.0	2.054493382476815E-5	0.0	3.4926387502105853E-4	8.628872206402624E-4
23	0.0	2.054493382476815E-5	0.0	7.190726838668853E-4	9.039770882897986E-4
24	0.0	2.054493382476815E-5	0.0	0.0011094264265374802	9.245220221145668E-4
25	0.0	2.054493382476815E-5	0.0	0.0014381453677337706	9.245220221145668E-4
26	0.0	2.054493382476815E-5	0.0	0.001787409242754829	9.245220221145668E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	1295	0.0	17.667744	29
GTATCAA	8445	0.0	17.219858	1
TCTAGCG	1350	0.0	17.065588	28
TAGCGGC	1375	0.0	16.872526	30
ATCGTTT	990	0.0	14.86851	29
AGCGGCG	1595	0.0	14.645593	31
AAGACGG	1430	0.0	14.206702	5
CATCGTT	1055	0.0	13.800076	28
CTTTCGT	1555	0.0	13.786484	27
TTCGTTC	1545	0.0	13.773299	29
GTATTAG	1395	0.0	13.662061	1
CGGTCCA	1780	0.0	13.573988	10
AACGCAG	10635	0.0	13.4169235	6
TAGGACG	765	0.0	13.382986	4
GTCCTAT	1725	0.0	13.276707	1
CAAGACG	1560	0.0	13.228166	4
CGCCGGT	1795	0.0	13.190553	7
TTTCGTT	1645	0.0	13.1298685	28
TACCGTC	1085	0.0	13.122786	7
TCCTATA	355	0.0	13.073759	2
>>END_MODULE