FastQCFastQC Report
Thu 2 Feb 2017
SRR4062783_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062783_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4867380
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT118330.24310820194848154No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT81620.16768774987775764No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC63130.12970016723576133No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC63080.1295974425666375No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG61370.12608425888260213No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA60960.12524191659578665No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT58750.12070148622051288No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA57840.11883189724245899No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG56200.11546252809519701No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA54510.11199043427881118No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC53890.11071664838167557No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG51650.1061145832049275No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC50210.10315611273416089No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT49730.10216995591057201No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA81500.017.1011281
TCTAGCG12400.016.12818728
CTAGCGG12700.015.99916429
TAGCGGC13800.015.07167530
ATCGTTT10800.014.51775829
CATCGTT11100.014.26952328
ACCGTCG13000.014.1558698
TACCGTC13450.013.9203477
AAGACGG15850.013.8317725
ATACCGT14350.013.8280426
CGGTCCA14950.013.69982710
CAAGACG17100.013.6619284
CGCCGGT15150.013.6258497
CGTCGTA14050.013.55242310
TAGGACG9800.013.5521214
GTATTAG12050.013.5449481
GCCGGTC15600.013.2326598
GCGCAAG16750.013.0878991
CCGGTCC15700.013.0457799
CGTCTTA5400.013.03635515
Produced by FastQC (version 0.11.5)