##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062783_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4867380
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.211804708077036	32.0	32.0	32.0	32.0	32.0
2	31.285513767160158	32.0	32.0	32.0	32.0	32.0
3	31.374536198118907	32.0	32.0	32.0	32.0	32.0
4	31.47472110252333	32.0	32.0	32.0	32.0	32.0
5	31.39848645472513	32.0	32.0	32.0	32.0	32.0
6	34.91805303880116	36.0	36.0	36.0	36.0	36.0
7	34.946976812987685	36.0	36.0	36.0	36.0	36.0
8	34.8832273214748	36.0	36.0	36.0	32.0	36.0
9	34.99207684627048	36.0	36.0	36.0	36.0	36.0
10	34.842954320394135	36.0	36.0	36.0	32.0	36.0
11	35.02083276834765	36.0	36.0	36.0	36.0	36.0
12	34.912669649791056	36.0	36.0	36.0	32.0	36.0
13	34.973377463851186	36.0	36.0	36.0	36.0	36.0
14	34.920492338794176	36.0	36.0	36.0	32.0	36.0
15	34.890374698503095	36.0	36.0	36.0	32.0	36.0
16	34.902144891091304	36.0	36.0	36.0	32.0	36.0
17	34.864559989152276	36.0	36.0	36.0	32.0	36.0
18	34.87104643565943	36.0	36.0	36.0	32.0	36.0
19	34.86463086917397	36.0	36.0	36.0	32.0	36.0
20	34.853868405589864	36.0	36.0	36.0	32.0	36.0
21	34.847822237014576	36.0	36.0	36.0	32.0	36.0
22	34.833174931893545	36.0	36.0	36.0	32.0	36.0
23	34.776748682042495	36.0	36.0	36.0	32.0	36.0
24	34.753444358155726	36.0	36.0	36.0	32.0	36.0
25	34.7335383717729	36.0	36.0	36.0	32.0	36.0
26	34.67141912075901	36.0	36.0	36.0	32.0	36.0
27	34.65679688045725	36.0	36.0	36.0	32.0	36.0
28	34.62786961363197	36.0	36.0	36.0	32.0	36.0
29	34.59716130649343	36.0	36.0	36.0	32.0	36.0
30	34.57498346132827	36.0	36.0	36.0	32.0	36.0
31	34.57360921070473	36.0	36.0	36.0	32.0	36.0
32	34.54752926625823	36.0	36.0	36.0	32.0	36.0
33	34.51731116124075	36.0	36.0	36.0	32.0	36.0
34	34.51128019591649	36.0	36.0	36.0	32.0	36.0
35	34.482283076316214	36.0	36.0	36.0	32.0	36.0
36	34.4571892886933	36.0	36.0	36.0	32.0	36.0
37	34.445281855947144	36.0	36.0	36.0	32.0	36.0
38	34.01781430667012	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	8.0
20	30.0
21	113.0
22	354.0
23	1178.0
24	3218.0
25	7789.0
26	16609.0
27	31995.0
28	55749.0
29	87429.0
30	132750.0
31	192211.0
32	277032.0
33	446097.0
34	1092751.0
35	2522067.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.871326741363966	19.099609363763456	12.699437756681212	26.32962613819137
2	15.447433916165954	20.770916086648704	37.31505956595112	26.466590431234227
3	17.440717593448632	24.901178868302864	29.160965447530295	28.49713809071821
4	12.001758649948535	16.436412271925086	36.57669336828719	34.98513570983919
5	14.123024757888128	37.0323611603619	33.326238479937246	15.51837560181272
6	33.75894296941819	36.44947138814773	16.594481908200546	13.197103734233531
7	29.67944443006294	30.97690288908037	21.225707066784842	18.117945614071854
8	27.561388723269882	33.41642590844709	19.56646262313391	19.45572274514912
9	27.257936204642185	14.370022279805095	18.962242674825795	39.40979884072692
10	15.822567526116426	26.716898083326434	31.40050446455445	26.060029926002688
11	37.11959785655539	21.400010971582983	22.711258960382928	18.769132211478702
12	24.71225686521775	23.949811006454834	28.602495561250635	22.735436567076786
13	28.764657045726345	19.66797668309101	25.733882103452604	25.83348416773004
14	23.39798824007988	20.168365732693974	25.18223356302569	31.251412464200452
15	24.753768968110155	27.694591340721292	22.341670467479425	25.209969223689132
16	25.56622362877434	26.159540894596457	23.76753484781029	24.50670062881892
17	23.734349896658983	26.39325468732665	25.25658567853753	24.615809737476834
18	24.789373338428476	24.93403021748867	26.48071447061869	23.795881973464166
19	25.396270683612126	25.161565359597976	25.651048407973075	23.79111554881682
20	25.542673882047428	24.590950367548867	25.137342882618576	24.729032867785133
21	26.712329892874475	24.4669096724435	24.719201770811118	24.101558663870907
22	25.467228036784494	24.604936087753217	25.250992475002448	24.676843400459838
23	23.838295557316943	24.271383296208594	26.064526785901393	25.825794360573074
24	24.592552872387195	25.270617868339844	25.47923112639654	24.657598132876412
25	24.620257247542515	24.766907165958482	25.67304258928942	24.939792997209583
26	24.428527139554983	25.613805705288208	25.999290377839902	23.958376777316907
27	25.11155908234745	24.972264316543452	25.017011219186685	24.899165381922415
28	24.126449958704683	25.066462860923128	26.008448076788742	24.798639103583447
29	24.32912573088602	24.975921337557374	25.902497853054413	24.792455078502194
30	24.20106915835624	25.165263447686435	26.20323459438137	24.430432799575954
31	25.03042704699448	24.879503963117735	24.98857701679343	25.101491973094355
32	24.518180211941537	25.106874745756443	24.895200292559856	25.47974474974216
33	24.052858005744362	24.748858728926034	25.750485887684953	25.447797377644648
34	24.936475064613816	24.74275688358008	25.87700159017788	24.44376646162823
35	25.677736277011455	24.666802263229908	25.659697825113305	23.995763634645336
36	24.39462297991938	25.3235827077401	25.263817495243845	25.01797681709667
37	25.368843196956064	25.101265978822283	25.11086046291845	24.419030361303207
38	24.421315390739363	24.883659056472947	25.696972187267264	24.998053365520427
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	283.0
1	322.5
2	362.0
3	362.0
4	1243.5
5	2125.0
6	2125.0
7	2581.5
8	3038.0
9	3031.5
10	3025.0
11	3025.0
12	4260.5
13	5496.0
14	8097.0
15	10698.0
16	10698.0
17	16582.0
18	22466.0
19	22466.0
20	27947.0
21	33428.0
22	38301.0
23	43174.0
24	43174.0
25	50561.5
26	57949.0
27	57949.0
28	72878.0
29	87807.0
30	104744.5
31	121682.0
32	121682.0
33	155025.0
34	188368.0
35	188368.0
36	207876.5
37	227385.0
38	255327.5
39	283270.0
40	283270.0
41	297840.0
42	312410.0
43	349939.5
44	387469.0
45	387469.0
46	390172.5
47	392876.0
48	392876.0
49	420754.0
50	448632.0
51	450945.0
52	453258.0
53	453258.0
54	430095.5
55	406933.0
56	406933.0
57	391290.5
58	375648.0
59	335184.0
60	294720.0
61	294720.0
62	277935.5
63	261151.0
64	214955.5
65	168760.0
66	168760.0
67	140791.0
68	112822.0
69	112822.0
70	89843.5
71	66865.0
72	51732.5
73	36600.0
74	36600.0
75	26824.0
76	17048.0
77	17048.0
78	17387.0
79	17726.0
80	13800.5
81	9875.0
82	9875.0
83	9542.5
84	9210.0
85	9210.0
86	5797.0
87	2384.0
88	2049.0
89	1714.0
90	1714.0
91	1042.5
92	371.0
93	271.5
94	172.0
95	172.0
96	138.0
97	104.0
98	104.0
99	90.0
100	76.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009204130353496131
2	4.1089867649536296E-5
3	0.0
4	2.054493382476815E-4
5	4.1089867649536296E-5
6	1.8490440442291335E-4
7	3.698088088458267E-4
8	0.0024243021913226415
9	0.003944627294355485
10	8.423422868154941E-4
11	0.005362227728264488
12	3.698088088458267E-4
13	2.2599427207244967E-4
14	0.0
15	0.0
16	2.0544933824768148E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	1.2326960294860892E-4
22	6.163480147430446E-5
23	1.2326960294860892E-4
24	0.0
25	1.2326960294860892E-4
26	2.0544933824768148E-5
27	8.217973529907259E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.0272466912384075E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	4867380.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.439243293570904
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.18282007544524	29.912014427224292
2	15.023283701173535	12.45107016722537
3	5.170369176778997	6.427685587023742
4	2.410973127166301	3.996356079636232
5	1.3207144453972839	2.736470361207581
6	0.8037292057979244	1.9983558060725228
7	0.5595661771100963	1.6231599266483068
8	0.4003352777243981	1.3271672778096486
9	0.2837112427209098	1.0581101291009818
>10	1.6333965769482175	12.133441993229944
>50	0.10300721911680982	2.945179242551265
>100	0.07928067164301338	6.990563335108442
>500	0.015204146439605493	4.439258453296947
>1k	0.012960910988272152	10.243428350628925
>5k	5.981958917664002E-4	1.4733007848671253
>10k+	4.984965764720003E-5	0.24443807836868098
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	11833	0.24310820194848154	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	8162	0.16768774987775764	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	6313	0.12970016723576133	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	6308	0.1295974425666375	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	6137	0.12608425888260213	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	6096	0.12524191659578665	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	5875	0.12070148622051288	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	5784	0.11883189724245899	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	5620	0.11546252809519701	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	5451	0.11199043427881118	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	5389	0.11071664838167557	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	5165	0.1061145832049275	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	5021	0.10315611273416089	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	4973	0.10216995591057201	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.054493382476815E-5	0.0
5	0.0	0.0	0.0	2.054493382476815E-5	0.0
6	0.0	0.0	0.0	2.054493382476815E-5	4.10898676495363E-5
7	0.0	0.0	0.0	2.054493382476815E-5	4.10898676495363E-5
8	0.0	0.0	0.0	2.054493382476815E-5	4.10898676495363E-5
9	0.0	0.0	0.0	6.163480147430444E-5	4.10898676495363E-5
10	0.0	0.0	0.0	6.163480147430444E-5	4.10898676495363E-5
11	0.0	0.0	0.0	6.163480147430444E-5	4.10898676495363E-5
12	0.0	0.0	0.0	6.163480147430444E-5	3.9035374267059486E-4
13	0.0	0.0	0.0	6.163480147430444E-5	4.930784117944356E-4
14	0.0	0.0	0.0	6.163480147430444E-5	4.930784117944356E-4
15	0.0	0.0	0.0	6.163480147430444E-5	5.752581470935082E-4
16	0.0	0.0	0.0	6.163480147430444E-5	5.958030809182763E-4
17	0.0	0.0	0.0	6.163480147430444E-5	6.163480147430445E-4
18	0.0	0.0	0.0	8.21797352990726E-5	6.77982816217349E-4
19	0.0	0.0	0.0	8.21797352990726E-5	6.77982816217349E-4
20	0.0	0.0	0.0	1.232696029486089E-4	7.601625515164216E-4
21	0.0	0.0	0.0	2.465392058972178E-4	9.245220221145668E-4
22	0.0	0.0	0.0	3.698088088458267E-4	9.450669559393349E-4
23	0.0	0.0	0.0	7.396176176916534E-4	9.656118897641031E-4
24	0.0	0.0	0.0	0.0011299713603622483	9.861568235888711E-4
25	0.0	0.0	0.0	0.0013970555000842341	0.0010067017574136394
26	0.0	0.0	0.0	0.0017052295074557564	0.0010067017574136394
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	8150	0.0	17.101128	1
TCTAGCG	1240	0.0	16.128187	28
CTAGCGG	1270	0.0	15.999164	29
TAGCGGC	1380	0.0	15.071675	30
ATCGTTT	1080	0.0	14.517758	29
CATCGTT	1110	0.0	14.269523	28
ACCGTCG	1300	0.0	14.155869	8
TACCGTC	1345	0.0	13.920347	7
AAGACGG	1585	0.0	13.831772	5
ATACCGT	1435	0.0	13.828042	6
CGGTCCA	1495	0.0	13.699827	10
CAAGACG	1710	0.0	13.661928	4
CGCCGGT	1515	0.0	13.625849	7
CGTCGTA	1405	0.0	13.552423	10
TAGGACG	980	0.0	13.552121	4
GTATTAG	1205	0.0	13.544948	1
GCCGGTC	1560	0.0	13.232659	8
GCGCAAG	1675	0.0	13.087899	1
CCGGTCC	1570	0.0	13.045779	9
CGTCTTA	540	0.0	13.036355	15
>>END_MODULE