FastQCFastQC Report
Thu 2 Feb 2017
SRR4062782_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062782_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4258283
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT66820.15691770603315938No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15850.021.2248121
GTATCAA43200.020.8774991
CTAGCGG6300.017.26935429
TCTAGCG6250.017.15050928
TAGCGGC6750.015.16976430
CGCCGGT6900.014.8391147
TATCAAC61650.014.4625242
TCAACGC61700.014.2079594
TAGGACG3500.014.1686984
CAACGCA62550.014.0917745
ATCAACG62250.014.0844123
AACGCAG65100.013.5643656
CAAGACG9450.013.5423654
AAGACGG9750.013.2899045
CGCGTAA4000.013.19978210
GTATTAG6050.012.9746151
CCGGTCC9150.012.9396769
AACGAAT4000.012.79948931
ACCGTCG4950.012.6048168
TCGCGTA4200.012.571229
Produced by FastQC (version 0.11.5)