##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062782_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4258283
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.31482830051455	32.0	32.0	32.0	32.0	32.0
2	30.989401596840793	32.0	32.0	32.0	32.0	32.0
3	31.023094284715224	32.0	32.0	32.0	32.0	32.0
4	31.093239927924003	32.0	32.0	32.0	32.0	32.0
5	31.014911878801854	32.0	32.0	32.0	32.0	32.0
6	34.663802758059994	36.0	36.0	36.0	32.0	36.0
7	34.62811795270535	36.0	36.0	36.0	32.0	36.0
8	34.60817188524107	36.0	36.0	36.0	32.0	36.0
9	34.74642314754562	36.0	36.0	36.0	32.0	36.0
10	34.50116889835645	36.0	36.0	36.0	32.0	36.0
11	34.70366436425198	36.0	36.0	36.0	32.0	36.0
12	34.570014956732564	36.0	36.0	36.0	32.0	36.0
13	34.62235318789287	36.0	36.0	36.0	32.0	36.0
14	34.54582069815463	36.0	36.0	36.0	32.0	36.0
15	34.4959599913862	36.0	36.0	36.0	32.0	36.0
16	34.51254883717217	36.0	36.0	36.0	32.0	36.0
17	34.455683194376704	36.0	36.0	36.0	32.0	36.0
18	34.47408826515288	36.0	36.0	36.0	32.0	36.0
19	34.46224006248528	36.0	36.0	36.0	32.0	36.0
20	34.450685170525304	36.0	36.0	36.0	32.0	36.0
21	34.43892056962865	36.0	36.0	36.0	32.0	36.0
22	34.414306658340934	36.0	36.0	36.0	32.0	36.0
23	34.36496376591222	36.0	36.0	36.0	32.0	36.0
24	34.35207805587369	36.0	36.0	36.0	32.0	36.0
25	34.32526701489779	36.0	36.0	36.0	32.0	36.0
26	34.284959219478836	36.0	36.0	36.0	32.0	36.0
27	34.296296418063335	36.0	36.0	36.0	32.0	36.0
28	34.27473420625167	36.0	36.0	36.0	32.0	36.0
29	34.24505745625643	36.0	36.0	36.0	32.0	36.0
30	34.22621112781842	36.0	36.0	36.0	32.0	36.0
31	34.2342357236473	36.0	36.0	36.0	32.0	36.0
32	34.20648745045832	36.0	36.0	36.0	32.0	36.0
33	34.17947233662018	36.0	36.0	36.0	32.0	36.0
34	34.183777592987596	36.0	36.0	36.0	32.0	36.0
35	34.15514422127416	36.0	36.0	36.0	32.0	36.0
36	34.11820726804677	36.0	36.0	36.0	32.0	36.0
37	34.113456057288815	36.0	36.0	36.0	32.0	36.0
38	33.5503861532923	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	4.0
8	10.0
9	16.0
10	33.0
11	49.0
12	25.0
13	19.0
14	299.0
15	839.0
16	1141.0
17	1279.0
18	1574.0
19	2018.0
20	2704.0
21	3902.0
22	6047.0
23	9412.0
24	14734.0
25	23022.0
26	34182.0
27	49910.0
28	71519.0
29	98550.0
30	135480.0
31	184425.0
32	256486.0
33	390142.0
34	901752.0
35	2068709.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.82380685906148	16.697812868852075	11.171532966852102	26.30684730523435
2	16.88661241411331	18.566774861615574	37.662110323132744	26.88450240113837
3	19.469857514313276	22.092926305633405	28.269177156726272	30.168039023327047
4	12.897072914122532	14.761892460593307	35.44458169725891	36.896452928025255
5	14.850269111444945	35.884524078188505	34.09168458668573	15.173522223680822
6	35.617828076740984	34.943094288011665	16.065131583891308	13.373946051356041
7	31.0760301924579	29.9241337234124	20.162920163577706	18.836915920552
8	28.241988640446603	33.81310608590445	18.593295956617084	19.351609317031862
9	26.992754814389674	14.408413170024675	18.14570027870434	40.45313173688131
10	15.5259514205789	26.8475838980392	32.01518271295532	25.611281968426567
11	37.94565296522701	21.076678119106944	21.721388665137063	19.25628025052898
12	24.531470935733616	23.258599298712333	28.87026272102527	23.339667044528785
13	29.723379998788076	19.406803983951832	24.90409100538744	25.96572501187266
14	23.320009854630115	19.11398984052456	25.02862328515697	32.53737701968836
15	25.19282409669345	27.136380121618476	21.924726959763678	25.7460688219244
16	25.887750354466014	25.855809073813248	23.446238714570132	24.810201857150606
17	24.32106322368248	25.88857215873004	24.963616223265323	24.826748394322156
18	24.898204385959556	25.301300456876657	25.54977146773094	24.250723689432842
19	25.403803568337715	25.09621416320393	25.189827308875707	24.310154959582654
20	25.608989684446072	24.704723022539028	24.98717718685328	24.69911010616162
21	26.34325482145713	24.361595369841734	24.595625595790697	24.699524212910436
22	25.70629735226563	24.367047285332482	25.042766757946254	24.883888604455635
23	24.89255831114512	24.458939883618438	25.227912128328324	25.420589676908122
24	24.88795504391373	24.857891624190344	25.12902609082065	25.125127241075273
25	25.10852454996545	24.57037619182058	25.161841448227882	25.15925780998609
26	24.75736684066729	25.320872370730314	25.367895757466428	24.553865031135967
27	25.144231525741144	24.998949015139114	24.98147565789891	24.875343801220833
28	24.90532582594043	24.811526470605504	25.27826868090253	25.004879022551535
29	24.76601887068083	25.07697597504076	25.276655529517207	24.880349624761205
30	24.846664739383485	25.146489569538716	25.32022686831591	24.68661882276189
31	25.250678951516615	24.801903976387234	24.768766633255332	25.178650438840815
32	24.90519607919119	24.96531821637347	24.821494791332736	25.307990913102614
33	24.749219805388183	24.811950082575684	25.111862340038964	25.326967771997168
34	25.213287684777892	24.73808331025775	25.35620364479148	24.69242536017288
35	25.266588296914104	24.73088680346534	25.349522441660138	24.653002457960422
36	24.69706859211203	25.00627694123068	25.119015268292554	25.17763919836473
37	25.312662860530207	24.6851646871516	25.02317478189166	24.978997670426537
38	24.796390058381608	24.872152989453546	25.142795276394963	25.18866167576988
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	40.0
1	107.5
2	175.0
3	175.0
4	522.0
5	869.0
6	869.0
7	1089.0
8	1309.0
9	1277.5
10	1246.0
11	1246.0
12	1889.5
13	2533.0
14	4069.5
15	5606.0
16	5606.0
17	8935.5
18	12265.0
19	12265.0
20	16624.5
21	20984.0
22	24582.0
23	28180.0
24	28180.0
25	34821.0
26	41462.0
27	41462.0
28	52234.5
29	63007.0
30	77174.0
31	91341.0
32	91341.0
33	113768.5
34	136196.0
35	136196.0
36	156125.0
37	176054.0
38	201922.5
39	227791.0
40	227791.0
41	248373.0
42	268955.0
43	302876.0
44	336797.0
45	336797.0
46	352400.0
47	368003.0
48	368003.0
49	384392.5
50	400782.0
51	407862.5
52	414943.0
53	414943.0
54	403170.0
55	391397.0
56	391397.0
57	372571.0
58	353745.0
59	319053.0
60	284361.0
61	284361.0
62	256490.0
63	228619.0
64	191126.0
65	153633.0
66	153633.0
67	126999.5
68	100366.0
69	100366.0
70	80351.5
71	60337.0
72	47136.0
73	33935.0
74	33935.0
75	26752.5
76	19570.0
77	19570.0
78	16829.5
79	14089.0
80	10955.0
81	7821.0
82	7821.0
83	6687.0
84	5553.0
85	5553.0
86	3839.0
87	2125.0
88	1664.0
89	1203.0
90	1203.0
91	866.5
92	530.0
93	487.5
94	445.0
95	445.0
96	409.0
97	373.0
98	373.0
99	1008.0
100	1643.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12265507013037884
2	0.0560084898068071
3	0.012258461919980423
4	0.0028884881535586058
5	2.3483643524866714E-4
6	2.5832007877353385E-4
7	2.3483643524866714E-5
8	3.9922193992273416E-4
9	1.64385504674067E-4
10	4.461892269724675E-4
11	0.0021839788478126045
12	0.002724102648884539
13	0.014113669758444893
14	0.009064686400598551
15	0.024305571048237048
16	0.011037312456687355
17	0.02092392638065624
18	0.005659558089492878
19	0.007843536937305483
20	0.005800459950642078
21	0.00671632204811188
22	0.007115543988034614
23	0.009205588261747751
24	0.014489408054842761
25	0.0169317069814289
26	0.01942097319506477
27	0.008735915391250418
28	0.006058780029415612
29	0.010825959664963554
30	0.0030528736582326726
31	0.005800459950642078
32	0.006669354761062146
33	0.008712431747725551
34	0.012587232929328559
35	0.016156746745108298
36	0.014935597281815228
37	0.009910097567493753
38	0.006199681890564812
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	4258283.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.39871511029055
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.79752867963632	27.473319583387823
2	15.842296947369094	13.43386069928256
3	7.260690292778224	9.235318176826683
4	3.806249352178357	6.4552032768695256
5	2.3130959763486003	4.9036148661981835
6	1.4558010501653018	3.703445638993226
7	0.980825880063457	2.9110029943127365
8	0.6934816779732902	2.352218567887663
9	0.48246021900252106	1.8410124039782654
>10	2.2047718848565503	15.761826393982197
>50	0.09722156236969345	2.819944896590843
>100	0.05540363779569139	4.743850189595763
>500	0.006837482477156229	1.9293229568088277
>1k	0.003279767702722877	2.2785703499642476
>5k	5.558928309699574E-5	0.1574890053214938
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	6682	0.15691770603315938	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.5832007877353385E-4
2	0.0	0.0	0.0	0.0	2.5832007877353385E-4
3	0.0	0.0	0.0	0.0	2.5832007877353385E-4
4	0.0	0.0	0.0	0.0	2.5832007877353385E-4
5	0.0	0.0	0.0	0.0	3.522546528730007E-4
6	0.0	0.0	0.0	0.0	4.6967287049733423E-4
7	0.0	0.0	0.0	2.348364352486671E-5	4.6967287049733423E-4
8	0.0	0.0	0.0	2.348364352486671E-5	4.6967287049733423E-4
9	0.0	0.0	0.0	2.348364352486671E-5	4.6967287049733423E-4
10	0.0	0.0	0.0	2.348364352486671E-5	4.93156514022201E-4
11	0.0	0.0	0.0	2.348364352486671E-5	5.166401575470677E-4
12	0.0	0.0	0.0	2.348364352486671E-5	0.0011272148891936023
13	0.0	0.0	0.0	2.348364352486671E-5	0.001221149463293069
14	0.0	0.0	0.0	2.348364352486671E-5	0.001221149463293069
15	0.0	0.0	0.0	4.696728704973342E-5	0.001549920472641203
16	0.0	0.0	0.0	4.696728704973342E-5	0.0016673386902655365
17	0.0	0.0	0.0	7.045093057460014E-5	0.0017143059773152701
18	0.0	0.0	0.0	9.393457409946684E-5	0.0018082405514147368
19	0.0	0.0	0.0	1.409018611492003E-4	0.0018082405514147368
20	0.0	0.0	0.0	2.3483643524866712E-4	0.002019593343138537
21	0.0	0.0	0.0	3.522546528730007E-4	0.0021135279172380043
22	0.0	0.0	0.0	3.992219399227341E-4	0.0021839788478126045
23	0.0	0.0	0.0	5.401238010719344E-4	0.002207462491337471
24	0.0	0.0	0.0	6.340583751714013E-4	0.002207462491337471
25	0.0	0.0	0.0	7.279929492708681E-4	0.002207462491337471
26	0.0	0.0	0.0	8.21927523370335E-4	0.0022544297783872046
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1585	0.0	21.224812	1
GTATCAA	4320	0.0	20.877499	1
CTAGCGG	630	0.0	17.269354	29
TCTAGCG	625	0.0	17.150509	28
TAGCGGC	675	0.0	15.169764	30
CGCCGGT	690	0.0	14.839114	7
TATCAAC	6165	0.0	14.462524	2
TCAACGC	6170	0.0	14.207959	4
TAGGACG	350	0.0	14.168698	4
CAACGCA	6255	0.0	14.091774	5
ATCAACG	6225	0.0	14.084412	3
AACGCAG	6510	0.0	13.564365	6
CAAGACG	945	0.0	13.542365	4
AAGACGG	975	0.0	13.289904	5
CGCGTAA	400	0.0	13.199782	10
GTATTAG	605	0.0	12.974615	1
CCGGTCC	915	0.0	12.939676	9
AACGAAT	400	0.0	12.799489	31
ACCGTCG	495	0.0	12.604816	8
TCGCGTA	420	0.0	12.57122	9
>>END_MODULE