FastQCFastQC Report
Thu 2 Feb 2017
SRR4062782_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062782_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4258283
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT60880.14296842177938854No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38400.021.3343521
TCTAGCG5600.019.71383928
GGTATCA13950.018.8109321
CTAGCGG6050.018.51197629
TAGCGGC7850.015.08245930
TCAACGC54650.014.6387924
TATCAAC55650.014.43273452
ATCAACG55850.014.2954453
CAACGCA56850.014.1857065
TATACCG2150.014.1405415
AACGCAG58250.013.87223156
GTATAGA6450.013.6440611
AAGACGG10750.013.545155
CAAGACG10400.013.53863054
CCTATAC3900.013.5384713
ACCGTCG4900.013.3887088
CGTTTAT5900.013.28783331
ATACCGT5700.013.1939216
CGCCGGT8250.013.1888187
TACCGTC4750.013.1377777
Produced by FastQC (version 0.11.5)