##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062782_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4258283
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22655657221467	32.0	32.0	32.0	32.0	32.0
2	31.258032404140355	32.0	32.0	32.0	32.0	32.0
3	31.353851775469128	32.0	32.0	32.0	32.0	32.0
4	31.457433195492175	32.0	32.0	32.0	32.0	32.0
5	31.369008823509382	32.0	32.0	32.0	32.0	32.0
6	34.902282210928675	36.0	36.0	36.0	36.0	36.0
7	34.93750039628648	36.0	36.0	36.0	36.0	36.0
8	34.86304362579941	36.0	36.0	36.0	32.0	36.0
9	34.98682497147325	36.0	36.0	36.0	36.0	36.0
10	34.83205484464043	36.0	36.0	36.0	32.0	36.0
11	35.01041170819318	36.0	36.0	36.0	36.0	36.0
12	34.91846690320958	36.0	36.0	36.0	32.0	36.0
13	34.96822663031085	36.0	36.0	36.0	36.0	36.0
14	34.914538794157174	36.0	36.0	36.0	32.0	36.0
15	34.88398023334757	36.0	36.0	36.0	32.0	36.0
16	34.90049299212852	36.0	36.0	36.0	32.0	36.0
17	34.87038085538232	36.0	36.0	36.0	32.0	36.0
18	34.871929366836355	36.0	36.0	36.0	32.0	36.0
19	34.86677940381135	36.0	36.0	36.0	32.0	36.0
20	34.86149229630816	36.0	36.0	36.0	32.0	36.0
21	34.8523099568535	36.0	36.0	36.0	32.0	36.0
22	34.83292397428729	36.0	36.0	36.0	32.0	36.0
23	34.78010456327116	36.0	36.0	36.0	32.0	36.0
24	34.765171314353694	36.0	36.0	36.0	32.0	36.0
25	34.752928821311315	36.0	36.0	36.0	32.0	36.0
26	34.68708185904976	36.0	36.0	36.0	32.0	36.0
27	34.668394045205545	36.0	36.0	36.0	32.0	36.0
28	34.654152624426324	36.0	36.0	36.0	32.0	36.0
29	34.63067978337748	36.0	36.0	36.0	32.0	36.0
30	34.602431308581416	36.0	36.0	36.0	32.0	36.0
31	34.59598058654157	36.0	36.0	36.0	32.0	36.0
32	34.57722255660321	36.0	36.0	36.0	32.0	36.0
33	34.55473133185371	36.0	36.0	36.0	32.0	36.0
34	34.5500583216287	36.0	36.0	36.0	32.0	36.0
35	34.52556229823147	36.0	36.0	36.0	32.0	36.0
36	34.498617400487475	36.0	36.0	36.0	32.0	36.0
37	34.496479919253844	36.0	36.0	36.0	32.0	36.0
38	34.08322626748856	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	5.0
20	19.0
21	69.0
22	266.0
23	896.0
24	2538.0
25	6400.0
26	13732.0
27	26643.0
28	47240.0
29	75793.0
30	115104.0
31	166893.0
32	243367.0
33	392955.0
34	967725.0
35	2198636.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.0964588486978	17.874853595978685	11.907887181243188	27.12080037408033
2	15.924047321420396	19.52002720345266	36.872537593203646	27.6833878819233
3	18.453165278117964	22.84798826193562	28.519640427843807	30.179206032102613
4	12.445685216962064	15.598907445942364	35.366212500402156	36.589194836693416
5	14.395909709063215	36.418351169568744	33.65101253346715	15.534726587900888
6	35.74733060985244	35.32817036753841	15.693463739785773	13.231035282823377
7	30.97776069207432	30.10170090161007	20.040222897979014	18.8803155083366
8	28.21496927098133	34.011469661992535	18.357024933128866	19.416536133897267
9	26.907349461310048	14.182214260418007	17.913542822305118	40.99689345596683
10	15.248361940998642	27.072962756217883	32.07150558176487	25.607169721018607
11	38.10846894789559	21.01672465439276	21.577681046761633	19.29712535095002
12	24.4337019493832	23.351619319582838	28.8254384996724	23.389240231361562
13	29.750816821749098	19.413081312541493	24.832132650959675	26.003969214749734
14	23.24097446833593	19.072297013748035	24.970663044547788	32.716065473368246
15	25.211264728060584	27.23050581654625	21.786832861977466	25.7713965934157
16	25.93811916122961	25.967708565968284	23.27016465094718	24.82400762185492
17	24.395983075807784	25.983782665454598	24.784026801412683	24.836207457324935
18	24.918775947958366	25.39413186018872	25.41435127726363	24.27274091458928
19	25.435697909227734	25.179186071005617	25.0979561480531	24.287159871713552
20	25.65073763298494	24.68572426961759	24.831088023036514	24.832450074360956
21	26.393545181195393	24.451749957024862	24.45416877628411	24.700536085495635
22	25.72365434612965	24.47246930276828	24.906235682316087	24.897640668785986
23	24.96005421917623	24.44167493293054	25.082099029841213	25.51617181805202
24	24.8867912254775	24.81582365474535	25.030722476641408	25.266662643135746
25	25.102799770235762	24.59411997056087	25.134338340521683	25.168741918681683
26	24.82691959501968	25.258455231113214	25.241781836379516	24.672843337487592
27	25.194391821488534	24.977379369750377	24.89347227202714	24.934756536733953
28	24.89200459434002	24.881507405684403	25.212908583107325	25.013579416868254
29	24.827236705498436	25.02217443039835	25.172868970897426	24.977719893205784
30	24.933241872369685	25.11300916355254	25.216877318863023	24.73687164521475
31	25.235523331821767	24.80008961358369	24.719775552728645	25.244611501865894
32	24.979856904766546	24.92732399420142	24.751807242496564	25.341011858535474
33	24.84463808535036	24.75965077943387	25.0144717953222	25.38123933989357
34	25.20025089924742	24.741497922989147	25.32941093863419	24.728840239129248
35	25.323610478683545	24.68161463200074	25.222607327883097	24.772167561432624
36	24.80950655463716	24.997328735549047	25.015927781220743	25.17723692859305
37	25.358272336526248	24.648361792769528	24.936036425949144	25.057329444755084
38	24.841996101243673	24.80155724810082	25.101913189852898	25.25453346080261
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	19.0
1	56.0
2	93.0
3	93.0
4	346.5
5	600.0
6	600.0
7	803.5
8	1007.0
9	1079.0
10	1151.0
11	1151.0
12	1723.0
13	2295.0
14	3753.0
15	5211.0
16	5211.0
17	8548.5
18	11886.0
19	11886.0
20	15914.5
21	19943.0
22	24089.0
23	28235.0
24	28235.0
25	34915.5
26	41596.0
27	41596.0
28	52472.0
29	63348.0
30	77729.5
31	92111.0
32	92111.0
33	114610.0
34	137109.0
35	137109.0
36	156351.5
37	175594.0
38	201612.0
39	227630.0
40	227630.0
41	248501.0
42	269372.0
43	303106.0
44	336840.0
45	336840.0
46	351910.5
47	366981.0
48	366981.0
49	384292.0
50	401603.0
51	408642.5
52	415682.0
53	415682.0
54	404994.0
55	394306.0
56	394306.0
57	374533.0
58	354760.0
59	319742.5
60	284725.0
61	284725.0
62	257191.0
63	229657.0
64	191544.5
65	153432.0
66	153432.0
67	127157.0
68	100882.0
69	100882.0
70	80678.0
71	60474.0
72	47270.5
73	34067.0
74	34067.0
75	26502.5
76	18938.0
77	18938.0
78	16415.5
79	13893.0
80	10608.5
81	7324.0
82	7324.0
83	6059.0
84	4794.0
85	4794.0
86	3224.0
87	1654.0
88	1221.5
89	789.0
90	789.0
91	472.5
92	156.0
93	111.5
94	67.0
95	67.0
96	52.0
97	37.0
98	37.0
99	29.5
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008782882678300151
2	0.0
3	0.0
4	9.393457409946686E-5
5	9.393457409946686E-5
6	1.64385504674067E-4
7	2.818037222984005E-4
8	0.0021839788478126045
9	0.004109637616851675
10	9.863130280444018E-4
11	0.004649761417923609
12	3.0528736582326725E-4
13	2.3483643524866714E-4
14	4.696728704973343E-5
15	0.0
16	4.696728704973343E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	1.64385504674067E-4
22	0.0
23	2.5832007877353385E-4
24	0.0
25	1.1741821762433357E-4
26	4.696728704973343E-5
27	4.696728704973343E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	4.696728704973343E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	4258283.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.23188782001492
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.60269407067101	24.78392677261935
2	17.361293647731518	13.969552368917443
3	7.770486585063131	9.378640337915725
4	4.184856924722304	6.734587773529614
5	2.493478343765729	5.015867050400971
6	1.5912949262755165	3.841247937748529
7	1.0711736201291389	3.0166735844556354
8	0.7366219224329357	2.370855243926848
9	0.5641540130398501	2.042728286924744
>10	2.448001542099493	16.489884825847888
>50	0.10477775230782996	2.893565355351116
>100	0.06000718829240987	4.8882366853976515
>500	0.007576688546908853	2.065552103768499
>1k	0.0035240409070692678	2.364823432079027
>5k	5.8734015117820126E-5	0.1438582411168985
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	6088	0.14296842177938854	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.878691481989337E-4
2	0.0	0.0	0.0	0.0	1.878691481989337E-4
3	0.0	0.0	0.0	0.0	1.878691481989337E-4
4	0.0	0.0	0.0	0.0	1.878691481989337E-4
5	0.0	0.0	0.0	0.0	1.878691481989337E-4
6	0.0	0.0	0.0	0.0	2.1135279172380042E-4
7	0.0	0.0	0.0	0.0	2.1135279172380042E-4
8	0.0	0.0	0.0	2.348364352486671E-5	2.1135279172380042E-4
9	0.0	0.0	0.0	2.348364352486671E-5	2.1135279172380042E-4
10	0.0	0.0	0.0	2.348364352486671E-5	2.3483643524866712E-4
11	0.0	0.0	0.0	2.348364352486671E-5	2.3483643524866712E-4
12	0.0	0.0	0.0	2.348364352486671E-5	7.045093057460014E-4
13	0.0	0.0	0.0	2.348364352486671E-5	9.158620974698018E-4
14	0.0	0.0	0.0	2.348364352486671E-5	9.158620974698018E-4
15	0.0	0.0	0.0	7.045093057460014E-5	0.0012446331068179358
16	0.0	0.0	0.0	7.045093057460014E-5	0.0012681167503428026
17	0.0	0.0	0.0	9.393457409946684E-5	0.0012681167503428026
18	0.0	0.0	0.0	1.1741821762433356E-4	0.0012681167503428026
19	0.0	0.0	0.0	1.878691481989337E-4	0.0012916003938676691
20	0.0	0.0	0.0	3.0528736582326725E-4	0.00164385504674067
21	0.0	0.0	0.0	3.992219399227341E-4	0.0016908223337904033
22	0.0	0.0	0.0	4.4618922697246756E-4	0.0018082405514147368
23	0.0	0.0	0.0	5.401238010719344E-4	0.0018082405514147368
24	0.0	0.0	2.348364352486671E-5	6.810256622211346E-4	0.0018082405514147368
25	0.0	0.0	2.348364352486671E-5	7.984438798454682E-4	0.0018082405514147368
26	0.0	0.0	2.348364352486671E-5	9.393457409946685E-4	0.002043076986663404
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3840	0.0	21.334352	1
TCTAGCG	560	0.0	19.713839	28
GGTATCA	1395	0.0	18.810932	1
CTAGCGG	605	0.0	18.511976	29
TAGCGGC	785	0.0	15.082459	30
TCAACGC	5465	0.0	14.638792	4
TATCAAC	5565	0.0	14.4327345	2
ATCAACG	5585	0.0	14.295445	3
CAACGCA	5685	0.0	14.185706	5
TATACCG	215	0.0	14.140541	5
AACGCAG	5825	0.0	13.8722315	6
GTATAGA	645	0.0	13.644061	1
AAGACGG	1075	0.0	13.54515	5
CAAGACG	1040	0.0	13.5386305	4
CCTATAC	390	0.0	13.538471	3
ACCGTCG	490	0.0	13.388708	8
CGTTTAT	590	0.0	13.287833	31
ATACCGT	570	0.0	13.193921	6
CGCCGGT	825	0.0	13.188818	7
TACCGTC	475	0.0	13.137777	7
>>END_MODULE