FastQCFastQC Report
Thu 2 Feb 2017
SRR4062781_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062781_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3399427
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC55310.16270389097927387No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT52230.15364354051432785No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG48360.14225926898856778No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA47980.14114143354159392No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC45060.1325517506332685No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT45010.13240466702182457No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG44640.1313162482971395No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT44040.12955124495981235No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC43710.1285804931242824No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG40700.11972605971535791No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC39420.11596071926239333No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA39410.11593130254010456No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG37410.11004795808234741No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA37130.10922428985826141No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC36700.10795937079984361No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG36040.10601786712878375No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT35770.10522361562698655No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG35390.1041057801800127No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC35280.10378219623483605No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG34790.10234077684268554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG11450.016.76758828
CGCTTCG9050.016.61855532
CGCAAGA11950.016.608882
AACGCTT9300.016.51590230
CAAGACG12150.016.194214
TAGCGGC12000.016.13311230
CTAGCGG12000.016.13311229
ATCGTTT7500.015.99978129
TAAACGC9250.015.73962928
ACGCTTC9700.015.66988831
AAGACGG12900.015.6251625
AACCGCG7400.015.5662077
GCGCAAG12800.015.516451
CATCGTT7950.015.29471128
ACCGTCG7350.015.2369888
GTATCAA58700.015.1711091
TAGCGTA7400.015.1338127
AAACGCT9850.015.10639129
CGCGGTC7650.015.05883710
GTAAACG9700.015.00921827
Produced by FastQC (version 0.11.5)