##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062781_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3399427
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.263022856499052	32.0	32.0	32.0	32.0	32.0
2	30.846812712848372	32.0	32.0	32.0	32.0	32.0
3	30.903509032551664	32.0	32.0	32.0	32.0	32.0
4	30.9731098799886	32.0	32.0	32.0	32.0	32.0
5	30.875255447462177	32.0	32.0	32.0	32.0	32.0
6	34.54311888444729	36.0	36.0	36.0	32.0	36.0
7	34.487071203470464	36.0	36.0	36.0	32.0	36.0
8	34.46540784667533	36.0	36.0	36.0	32.0	36.0
9	34.6159788105466	36.0	36.0	36.0	32.0	36.0
10	34.30768038260565	36.0	36.0	36.0	32.0	36.0
11	34.58813617706749	36.0	36.0	36.0	32.0	36.0
12	34.400494259767896	36.0	36.0	36.0	32.0	36.0
13	34.4948834024087	36.0	36.0	36.0	32.0	36.0
14	34.3848360326608	36.0	36.0	36.0	32.0	36.0
15	34.31818332913164	36.0	36.0	36.0	32.0	36.0
16	34.343255201538376	36.0	36.0	36.0	32.0	36.0
17	34.25840060692581	36.0	36.0	36.0	32.0	36.0
18	34.28571256273484	36.0	36.0	36.0	32.0	36.0
19	34.2817907253193	36.0	36.0	36.0	32.0	36.0
20	34.270214068429766	36.0	36.0	36.0	32.0	36.0
21	34.25802966205775	36.0	36.0	36.0	32.0	36.0
22	34.229110964877314	36.0	36.0	36.0	32.0	36.0
23	34.16458479620242	36.0	36.0	36.0	32.0	36.0
24	34.14844089901033	36.0	36.0	36.0	32.0	36.0
25	34.11697441951247	36.0	36.0	36.0	32.0	36.0
26	34.06356777186273	36.0	36.0	36.0	32.0	36.0
27	34.0719768360962	36.0	36.0	36.0	32.0	36.0
28	34.05026405920763	36.0	36.0	36.0	32.0	36.0
29	34.021463028916344	36.0	36.0	36.0	32.0	36.0
30	33.99458173392163	36.0	36.0	36.0	32.0	36.0
31	34.012624774704676	36.0	36.0	36.0	32.0	36.0
32	33.969208340111436	36.0	36.0	36.0	32.0	36.0
33	33.933115198531986	36.0	36.0	36.0	32.0	36.0
34	33.94868546963944	36.0	36.0	36.0	32.0	36.0
35	33.90472335484775	36.0	36.0	36.0	32.0	36.0
36	33.860300868352226	36.0	36.0	36.0	32.0	36.0
37	33.86585386301868	36.0	36.0	36.0	32.0	36.0
38	33.25851533214274	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	7.0
8	6.0
9	13.0
10	26.0
11	28.0
12	19.0
13	23.0
14	243.0
15	577.0
16	805.0
17	1027.0
18	1358.0
19	1786.0
20	2569.0
21	3957.0
22	6286.0
23	9879.0
24	15060.0
25	22658.0
26	33709.0
27	47868.0
28	67705.0
29	90007.0
30	121412.0
31	163546.0
32	222286.0
33	331031.0
34	726001.0
35	1529533.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.178737516088084	16.752489419415262	11.091604155069993	25.977168909426663
2	17.053631791683113	19.061700444991498	37.36383374936315	26.520834013962236
3	19.11657019292297	23.258934547406977	27.9691970491538	29.65529821051625
4	12.292050750045522	14.892594916582016	35.94752726054304	36.86782707282942
5	14.7741379411782	36.30542857310953	33.64250372122303	15.277929764489237
6	35.094233833105456	35.23649356242367	16.557255716864308	13.112016887606579
7	31.115753071253792	29.856658153464732	20.658340555140782	18.369248220140694
8	28.62446292213893	32.65062743166911	19.152095037556474	19.57281460863549
9	27.40538897970506	14.099732924949823	18.175841224632798	40.319036870712324
10	15.74668920781323	26.277389655214	31.31714210659311	26.65877903037966
11	38.62064076160196	20.803233313555104	21.559198920032323	19.01692700481061
12	24.95446914300793	23.34939428001234	28.50191934147275	23.19421723550698
13	29.43035349151944	18.886391385574957	25.28951587990409	26.393739243001512
14	23.695373648729152	19.591334803323807	24.59872643320394	32.1145651147431
15	25.487362772678203	26.93646799066335	21.940835902736648	25.6353333339218
16	26.452359980159184	25.342668871986145	23.331315124737646	24.873656023117018
17	24.456570510415776	25.623620995904655	24.83318077675279	25.086627716926778
18	25.601670724923164	24.363100843334827	25.75093925323962	24.28428917850239
19	25.796537460052875	24.91129480895545	24.8272451511114	24.464922579880273
20	25.895262100688743	24.06111034623156	24.602556815841716	25.441070737237986
21	27.31065836752249	23.985224871779636	23.927064572935134	24.777052187762745
22	25.907148070559018	24.024144525898322	24.802534245588	25.266173157954658
23	24.336314687952136	23.72236274854183	25.445599839488153	26.495722724017877
24	25.002287461035934	24.964511255632228	24.68801532231135	25.34518596102049
25	25.229083422112815	24.282357288640846	24.67893015062943	25.809629138616913
26	24.836156323711265	25.197693669477545	25.27869239285764	24.687457613953555
27	25.681357970610723	24.451808418092742	24.456250729965983	25.41058288133055
28	24.765424885805054	24.407726335750645	25.475085394314185	25.351763384130116
29	24.55035402403545	24.879268198324066	25.40603101501796	25.164346762622525
30	24.668764927604702	24.953115664501432	25.669346513997056	24.708772893896814
31	25.31927612310002	24.83099223005532	24.228742087965234	25.620989558879426
32	25.02643997471176	24.52123420478117	24.16623985168314	26.286085968823926
33	24.54498089665542	24.174210970235734	25.00356707558346	26.27724105752538
34	25.508413641172535	24.647514389509396	25.09820523894647	24.745866730371603
35	26.298220239349153	24.08667080135715	25.270794766752854	24.344314192540846
36	24.68876970105298	25.12311227356845	24.66193772746129	25.526180297917282
37	25.96307732861366	24.78003026679972	24.576182429251944	24.68070997533468
38	24.884260791465852	24.490495955415877	25.051180603176537	25.574062649941737
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	91.0
1	129.0
2	167.0
3	167.0
4	524.5
5	882.0
6	882.0
7	1079.5
8	1277.0
9	1284.0
10	1291.0
11	1291.0
12	1744.0
13	2197.0
14	3404.0
15	4611.0
16	4611.0
17	7058.5
18	9506.0
19	9506.0
20	12116.0
21	14726.0
22	17614.5
23	20503.0
24	20503.0
25	25785.5
26	31068.0
27	31068.0
28	39249.0
29	47430.0
30	57916.5
31	68403.0
32	68403.0
33	89240.5
34	110078.0
35	110078.0
36	125186.5
37	140295.0
38	161914.5
39	183534.0
40	183534.0
41	196126.0
42	208718.0
43	237532.0
44	266346.0
45	266346.0
46	276065.5
47	285785.0
48	285785.0
49	303119.0
50	320453.0
51	325007.5
52	329562.0
53	329562.0
54	313290.0
55	297018.0
56	297018.0
57	287638.5
58	278259.0
59	250068.5
60	221878.0
61	221878.0
62	211396.5
63	200915.0
64	167670.5
65	134426.0
66	134426.0
67	111262.0
68	88098.0
69	88098.0
70	70129.5
71	52161.0
72	40427.5
73	28694.0
74	28694.0
75	21127.5
76	13561.0
77	13561.0
78	13554.5
79	13548.0
80	10591.0
81	7634.0
82	7634.0
83	7749.0
84	7864.0
85	7864.0
86	5215.0
87	2566.0
88	2294.0
89	2022.0
90	2022.0
91	1483.5
92	945.0
93	821.5
94	698.0
95	698.0
96	650.5
97	603.0
98	603.0
99	1108.5
100	1614.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1193436423256037
2	0.05395026867763302
3	0.011825522360091862
4	0.002941672228878573
5	2.0591705602150006E-4
6	3.23583945176643E-4
7	2.9416722288785725E-5
8	3.8241738975421444E-4
9	2.353337783102858E-4
10	4.118341120430001E-4
11	0.001941503671059858
12	0.002471004672258001
13	0.014031776531750792
14	0.009178017354101146
15	0.022886209940675296
16	0.010972437413717078
17	0.02023870493468458
18	0.005412676901136574
19	0.007236513683041288
20	0.00585392773546836
21	0.005912761180045931
22	0.0067658461264207175
23	0.008854433408924504
24	0.013590525697419005
25	0.016355697592564864
26	0.017885367151581722
27	0.008707349797480576
28	0.005765677568602003
29	0.010707686913118004
30	0.0030593391180337155
31	0.005147926400537502
32	0.006412845458955288
33	0.008354349130015147
34	0.012443273528156364
35	0.01597328020281065
36	0.014620110977526506
37	0.00950160129927779
38	0.005589177234869289
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	3399427.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.82405163240041
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.32673314908168	34.05952057491166
2	14.309812706290037	13.11467192606028
3	4.997936503691199	6.870771012018128
4	2.181122751894204	3.997915263976131
5	1.188376556444491	2.722811434062328
6	0.7090981608271704	1.9496250440510656
7	0.45253517877376775	1.451589677832468
8	0.3052206093164074	1.118915596847021
9	0.21692898143105716	0.8946508361094714
>10	1.129619835778111	9.258506433295098
>50	0.08235879829670283	2.6131613972350474
>100	0.0745370706953682	7.17876343639135
>500	0.014669274693775627	4.722999467609014
>1k	0.010921178075204286	9.72764419773331
>5k	1.2924471094916316E-4	0.31845370186767014
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	5531	0.16270389097927387	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	5223	0.15364354051432785	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	4836	0.14225926898856778	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	4798	0.14114143354159392	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	4506	0.1325517506332685	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4501	0.13240466702182457	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	4464	0.1313162482971395	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	4404	0.12955124495981235	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	4371	0.1285804931242824	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	4070	0.11972605971535791	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	3942	0.11596071926239333	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	3941	0.11593130254010456	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	3741	0.11004795808234741	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	3713	0.10922428985826141	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	3670	0.10795937079984361	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	3604	0.10601786712878375	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	3577	0.10522361562698655	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	3539	0.1041057801800127	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	3528	0.10378219623483605	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	3479	0.10234077684268554	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4708361144392864E-4
2	0.0	0.0	0.0	0.0	1.4708361144392864E-4
3	0.0	0.0	0.0	0.0	1.4708361144392864E-4
4	0.0	0.0	0.0	0.0	1.4708361144392864E-4
5	2.941672228878573E-5	0.0	0.0	0.0	1.4708361144392864E-4
6	2.941672228878573E-5	0.0	0.0	0.0	2.3533377831028583E-4
7	2.941672228878573E-5	0.0	0.0	2.941672228878573E-5	2.3533377831028583E-4
8	2.941672228878573E-5	0.0	0.0	2.941672228878573E-5	2.3533377831028583E-4
9	2.941672228878573E-5	0.0	0.0	2.941672228878573E-5	2.3533377831028583E-4
10	2.941672228878573E-5	0.0	0.0	2.941672228878573E-5	3.23583945176643E-4
11	2.941672228878573E-5	0.0	0.0	2.941672228878573E-5	3.23583945176643E-4
12	2.941672228878573E-5	0.0	0.0	2.941672228878573E-5	8.530849463747861E-4
13	2.941672228878573E-5	0.0	0.0	2.941672228878573E-5	0.0010590020023962862
14	2.941672228878573E-5	0.0	0.0	2.941672228878573E-5	0.0010590020023962862
15	2.941672228878573E-5	0.0	0.0	5.883344457757146E-5	0.0012649190584177862
16	2.941672228878573E-5	0.0	0.0	1.1766688915514291E-4	0.0013531692252841435
17	2.941672228878573E-5	0.0	0.0	1.4708361144392864E-4	0.0013825859475729292
18	2.941672228878573E-5	0.0	0.0	1.4708361144392864E-4	0.0014708361144392863
19	2.941672228878573E-5	0.0	0.0	2.059170560215001E-4	0.0015002528367280721
20	2.941672228878573E-5	0.0	0.0	2.3533377831028583E-4	0.0016473364481720007
21	2.941672228878573E-5	0.0	0.0	3.23583945176643E-4	0.0016767531704607866
22	2.941672228878573E-5	0.0	0.0	4.118341120430002E-4	0.0017061698927495722
23	2.941672228878573E-5	0.0	0.0	6.177511680645003E-4	0.0017061698927495722
24	2.941672228878573E-5	0.0	0.0	9.70751835529929E-4	0.0017061698927495722
25	2.941672228878573E-5	0.0	0.0	0.0013237525029953577	0.0017061698927495722
26	5.883344457757146E-5	0.0	0.0	0.001735586615038358	0.0018238367819047151
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	1145	0.0	16.767588	28
CGCTTCG	905	0.0	16.618555	32
CGCAAGA	1195	0.0	16.60888	2
AACGCTT	930	0.0	16.515902	30
CAAGACG	1215	0.0	16.19421	4
TAGCGGC	1200	0.0	16.133112	30
CTAGCGG	1200	0.0	16.133112	29
ATCGTTT	750	0.0	15.999781	29
TAAACGC	925	0.0	15.739629	28
ACGCTTC	970	0.0	15.669888	31
AAGACGG	1290	0.0	15.625162	5
AACCGCG	740	0.0	15.566207	7
GCGCAAG	1280	0.0	15.51645	1
CATCGTT	795	0.0	15.294711	28
ACCGTCG	735	0.0	15.236988	8
GTATCAA	5870	0.0	15.171109	1
TAGCGTA	740	0.0	15.133812	7
AAACGCT	985	0.0	15.106391	29
CGCGGTC	765	0.0	15.058837	10
GTAAACG	970	0.0	15.009218	27
>>END_MODULE