FastQCFastQC Report
Thu 2 Feb 2017
SRR4062781_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062781_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3399427
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC59440.17485299728454237No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT53080.15614396190887464No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA50290.1479366963903034No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC49800.14649527699815293No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC46550.13693484225429758No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT45240.13308125163446663No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG44990.132345833577247No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG43400.12766857473333004No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT43270.12728615734357585No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG42140.12396206772494307No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC41630.12246181488821499No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG40530.11922597543644856No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA40410.11887297476898312No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG37630.11069512597270069No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC37280.1096655406925932No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG37130.10922428985826141No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC37050.10898895607995111No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA36030.10598845040649497No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT35830.10540011596071927No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG34430.10128177484028927No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA34290.10086994072824626No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG34290.10086994072824626No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG9500.019.3679429
TCTAGCG10050.018.46720328
ATCGTTT8350.018.39475329
TAGCGGC10450.017.76032430
CATCGTT8750.017.5538528
CGTTTAT8800.017.45411331
TATAACG703.6993425E-415.9998392
TCGTTTA9600.015.83294130
AGCGGCG11800.015.45724331
CGGCATC9850.015.43108925
GCATCGT9850.015.26865727
GTATTAG8750.015.1781051
GTTTTCG11750.015.11451928
ACCGTCG7600.014.7377778
GGCATCG10650.014.72263926
CGCAAGA12150.014.6171362
TAAACGC9300.014.45125428
TTTTCGG12150.014.35355429
CGACGTA1801.1823431E-1014.22186931
CGCATCG10350.014.221868513
Produced by FastQC (version 0.11.5)