##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062781_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3399427
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.207402600497083	32.0	32.0	32.0	32.0	32.0
2	31.246852778424127	32.0	32.0	32.0	32.0	32.0
3	31.362074255455404	32.0	32.0	32.0	32.0	32.0
4	31.467278750212902	32.0	32.0	32.0	32.0	32.0
5	31.380989796221538	32.0	32.0	32.0	32.0	32.0
6	34.91808060593741	36.0	36.0	36.0	36.0	36.0
7	34.945109278710795	36.0	36.0	36.0	36.0	36.0
8	34.87114769636177	36.0	36.0	36.0	32.0	36.0
9	34.99590813392963	36.0	36.0	36.0	36.0	36.0
10	34.83141923624187	36.0	36.0	36.0	32.0	36.0
11	35.02360044795785	36.0	36.0	36.0	36.0	36.0
12	34.908782862523594	36.0	36.0	36.0	32.0	36.0
13	34.97437803488647	36.0	36.0	36.0	36.0	36.0
14	34.914025804937125	36.0	36.0	36.0	32.0	36.0
15	34.88119821369896	36.0	36.0	36.0	32.0	36.0
16	34.895225577722364	36.0	36.0	36.0	32.0	36.0
17	34.86082507434341	36.0	36.0	36.0	32.0	36.0
18	34.86485340029364	36.0	36.0	36.0	32.0	36.0
19	34.86190349138252	36.0	36.0	36.0	32.0	36.0
20	34.845364527610094	36.0	36.0	36.0	32.0	36.0
21	34.84074257220408	36.0	36.0	36.0	32.0	36.0
22	34.825910660826075	36.0	36.0	36.0	32.0	36.0
23	34.7586381469583	36.0	36.0	36.0	32.0	36.0
24	34.7353121570194	36.0	36.0	36.0	32.0	36.0
25	34.712872198755846	36.0	36.0	36.0	32.0	36.0
26	34.64959565244378	36.0	36.0	36.0	32.0	36.0
27	34.63705236205984	36.0	36.0	36.0	32.0	36.0
28	34.60412475396589	36.0	36.0	36.0	32.0	36.0
29	34.56787070291552	36.0	36.0	36.0	32.0	36.0
30	34.54725340476498	36.0	36.0	36.0	32.0	36.0
31	34.54896133966107	36.0	36.0	36.0	32.0	36.0
32	34.52409626681202	36.0	36.0	36.0	32.0	36.0
33	34.491555782783394	36.0	36.0	36.0	32.0	36.0
34	34.48878708088157	36.0	36.0	36.0	32.0	36.0
35	34.45298692985612	36.0	36.0	36.0	32.0	36.0
36	34.42825599726071	36.0	36.0	36.0	32.0	36.0
37	34.42893670021448	36.0	36.0	36.0	32.0	36.0
38	33.96840849943241	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	10.0
21	62.0
22	256.0
23	751.0
24	2136.0
25	5364.0
26	11168.0
27	21856.0
28	38465.0
29	60856.0
30	92659.0
31	136399.0
32	198268.0
33	322360.0
34	787633.0
35	1721182.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.52394911159463	17.96924737079318	11.757352776774251	26.749450740837933
2	16.09421234813985	20.020815272691543	36.58725426373327	27.297718115435337
3	18.067015411714973	24.01766533006886	28.291679744851116	29.623639513365045
4	11.872234637742508	15.821174147699946	35.893780672520805	36.412810542036745
5	14.318921582326421	36.78904520617458	33.25638894813093	15.63564426336807
6	35.2185737293807	35.65008788250954	16.163586488891504	12.96775189921825
7	30.871897845862755	30.05472711162989	20.573146179398083	18.500228863109268
8	28.539161832154036	32.90279915854328	18.88123675663209	19.67680225267059
9	27.28749767599485	13.874193070110824	17.93567153746767	40.90263771642665
10	15.550610887754504	26.548326070770788	31.313965388567443	26.587097652907264
11	38.784144115733156	20.711459055302495	21.406226281658284	19.09817054730607
12	24.79304539572521	23.493263697863487	28.36348114985599	23.350209756555316
13	29.411441121461234	18.88129805596363	25.216463269344025	26.49079755323111
14	23.52261131067089	19.579240854414582	24.554873512506667	32.343274322407865
15	25.480617762934756	27.005610063107692	21.766021155918335	25.747751018039217
16	26.49912852908948	25.44968634401406	23.11749781212999	24.933687314766466
17	24.493539646534547	25.73816116657307	24.63332791085086	25.134971276041522
18	25.69774259014828	24.4432958848653	25.560278246892786	24.298683278093634
19	25.805525460614394	25.034601419592185	24.67621749194791	24.483655627845515
20	25.930840697564616	24.005045556206973	24.48086103922808	25.583252707000327
21	27.291866884468334	24.083498876132804	23.809128784502544	24.815505454896318
22	25.85657868610133	24.139729513079416	24.710914345808483	25.29277745501077
23	24.322635644958883	23.71606265293023	25.31642106638003	26.644880635730857
24	24.963383534931033	25.03736659148733	24.576965470945545	25.42228440263609
25	25.260661806235408	24.340388254010094	24.665384488666316	25.733565451088186
26	24.93827632614339	25.192172205593593	25.124278370606795	24.74527309765622
27	25.795980202534253	24.417688285518874	24.317671370893606	25.468660141053267
28	24.77017450293829	24.429881859501617	25.387572670335324	25.412370967224774
29	24.613824623973393	24.777087432676154	25.376364899143294	25.232723044207155
30	24.75270096989875	24.870838526610513	25.548040890420648	24.828419613070086
31	25.369481386127724	24.75776064613242	24.135508719557738	25.73724924818212
32	25.076490832131416	24.536429227631597	24.149128661977446	26.23795127825954
33	24.567257952590246	24.12794862192952	24.95597052091426	26.348822904565978
34	25.4828534338287	24.508071507345207	25.096788370510676	24.91228668831541
35	26.286841870703505	24.06996826229832	25.148473551572074	24.4947163154261
36	24.81497617098411	25.10305413235819	24.609088531684897	25.472881164972804
37	26.176646829009716	24.660744296024006	24.437912624686454	24.724696250279827
38	24.929833721879437	24.3489413650838	25.02743846385786	25.69378644917891
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	44.0
1	85.0
2	126.0
3	126.0
4	437.0
5	748.0
6	748.0
7	930.0
8	1112.0
9	1148.0
10	1184.0
11	1184.0
12	1655.5
13	2127.0
14	3320.5
15	4514.0
16	4514.0
17	6871.0
18	9228.0
19	9228.0
20	11758.5
21	14289.0
22	17271.5
23	20254.0
24	20254.0
25	25661.0
26	31068.0
27	31068.0
28	39319.5
29	47571.0
30	58028.5
31	68486.0
32	68486.0
33	89870.5
34	111255.0
35	111255.0
36	125536.5
37	139818.0
38	161299.5
39	182781.0
40	182781.0
41	195159.5
42	207538.0
43	236019.0
44	264500.0
45	264500.0
46	274683.0
47	284866.0
48	284866.0
49	303636.0
50	322406.0
51	326246.0
52	330086.0
53	330086.0
54	313722.0
55	297358.0
56	297358.0
57	288669.0
58	279980.0
59	251871.0
60	223762.0
61	223762.0
62	213286.5
63	202811.0
64	169317.5
65	135824.0
66	135824.0
67	112675.5
68	89527.0
69	89527.0
70	71348.0
71	53169.0
72	41113.5
73	29058.0
74	29058.0
75	21134.5
76	13211.0
77	13211.0
78	13194.5
79	13178.0
80	10302.0
81	7426.0
82	7426.0
83	7083.5
84	6741.0
85	6741.0
86	4242.5
87	1744.0
88	1492.5
89	1241.0
90	1241.0
91	731.5
92	222.0
93	153.5
94	85.0
95	85.0
96	65.5
97	46.0
98	46.0
99	44.5
100	43.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008913266853502076
2	0.0
3	0.0
4	2.353337783102858E-4
5	5.883344457757145E-5
6	5.883344457757145E-5
7	4.412508343317859E-4
8	0.002471004672258001
9	0.003618256841520644
10	8.825016686635718E-4
11	0.00467725884391693
12	2.941672228878573E-4
13	1.4708361144392864E-4
14	0.0
15	0.0
16	5.883344457757145E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	1.176668891551429E-4
22	5.883344457757145E-5
23	2.9416722288785725E-5
24	0.0
25	8.825016686635718E-5
26	5.883344457757145E-5
27	5.883344457757145E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	5.883344457757145E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	3399427.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.49360064938714
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.94088287807887	31.28968030263495
2	15.57697394259003	13.549973679698404
3	5.462747002375114	7.127836097098197
4	2.441158588393692	4.246991070616676
5	1.2907965081328487	2.8070693922176764
6	0.7900594680620503	2.061751859589483
7	0.48298427563913443	1.4704707643207426
8	0.33369003802755204	1.1610705003719324
9	0.23418661591876727	0.9167057235182045
>10	1.2488047531532398	9.680173515234106
>50	0.08984950719150192	2.703871601421488
>100	0.07974180641862334	7.366730188070826
>500	0.01566261909066406	4.784352517672419
>1k	0.012257701896256464	10.351105263335366
>5k	2.0429503160427438E-4	0.4822175241997102
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	5944	0.17485299728454237	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	5308	0.15614396190887464	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	5029	0.1479366963903034	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	4980	0.14649527699815293	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	4655	0.13693484225429758	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	4524	0.13308125163446663	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	4499	0.132345833577247	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	4340	0.12766857473333004	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4327	0.12728615734357585	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	4214	0.12396206772494307	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	4163	0.12246181488821499	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	4053	0.11922597543644856	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	4041	0.11887297476898312	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	3763	0.11069512597270069	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	3728	0.1096655406925932	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	3713	0.10922428985826141	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	3705	0.10898895607995111	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	3603	0.10598845040649497	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	3583	0.10540011596071927	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	3443	0.10128177484028927	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	3429	0.10086994072824626	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	3429	0.10086994072824626	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.7650033373271438E-4
2	0.0	0.0	0.0	0.0	1.7650033373271438E-4
3	0.0	0.0	0.0	0.0	1.7650033373271438E-4
4	0.0	0.0	0.0	0.0	1.7650033373271438E-4
5	0.0	0.0	0.0	0.0	1.7650033373271438E-4
6	0.0	0.0	0.0	0.0	2.6475050059907154E-4
7	0.0	0.0	0.0	0.0	2.6475050059907154E-4
8	0.0	0.0	0.0	0.0	2.6475050059907154E-4
9	0.0	0.0	0.0	2.941672228878573E-5	2.6475050059907154E-4
10	0.0	0.0	0.0	2.941672228878573E-5	2.6475050059907154E-4
11	0.0	0.0	0.0	2.941672228878573E-5	2.6475050059907154E-4
12	0.0	0.0	0.0	2.941672228878573E-5	5.589177234869288E-4
13	0.0	0.0	0.0	2.941672228878573E-5	7.354180572196431E-4
14	0.0	0.0	0.0	2.941672228878573E-5	7.354180572196431E-4
15	0.0	0.0	0.0	2.941672228878573E-5	9.70751835529929E-4
16	0.0	0.0	0.0	5.883344457757146E-5	0.0010001685578187147
17	0.0	0.0	0.0	5.883344457757146E-5	0.0010001685578187147
18	0.0	0.0	0.0	8.825016686635719E-5	0.0010590020023962862
19	0.0	0.0	0.0	8.825016686635719E-5	0.001088418724685072
20	0.0	0.0	0.0	1.1766688915514291E-4	0.0012060856138402147
21	0.0	0.0	0.0	1.7650033373271438E-4	0.0013531692252841435
22	0.0	0.0	0.0	2.6475050059907154E-4	0.0015296695590168578
23	0.0	0.0	0.0	4.7066755662057165E-4	0.0015296695590168578
24	0.0	0.0	0.0	7.060013349308575E-4	0.0015296695590168578
25	0.0	0.0	0.0	9.70751835529929E-4	0.0015296695590168578
26	0.0	0.0	0.0	0.0013531692252841435	0.001735586615038358
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	950	0.0	19.36794	29
TCTAGCG	1005	0.0	18.467203	28
ATCGTTT	835	0.0	18.394753	29
TAGCGGC	1045	0.0	17.760324	30
CATCGTT	875	0.0	17.55385	28
CGTTTAT	880	0.0	17.454113	31
TATAACG	70	3.6993425E-4	15.999839	2
TCGTTTA	960	0.0	15.832941	30
AGCGGCG	1180	0.0	15.457243	31
CGGCATC	985	0.0	15.431089	25
GCATCGT	985	0.0	15.268657	27
GTATTAG	875	0.0	15.178105	1
GTTTTCG	1175	0.0	15.114519	28
ACCGTCG	760	0.0	14.737777	8
GGCATCG	1065	0.0	14.722639	26
CGCAAGA	1215	0.0	14.617136	2
TAAACGC	930	0.0	14.451254	28
TTTTCGG	1215	0.0	14.353554	29
CGACGTA	180	1.1823431E-10	14.221869	31
CGCATCG	1035	0.0	14.2218685	13
>>END_MODULE