FastQCFastQC Report
Thu 2 Feb 2017
SRR4062780_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062780_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3323551
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT89580.26953099260399493No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT51290.15432289138936037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA57700.016.6538731
TTACGCT600.00244585715.9974054
TCTAGCG5550.014.98991328
TCAATCG650.00416410614.76905725
CTAGCGG5900.014.64389529
GTATTAC2550.014.4453321
AACGCAG67700.014.0122876
GTCCTAT8950.013.4207891
GTATTAG5750.013.369441
ATACCGT5300.013.2806646
GTATAGG2900.013.2541871
TAGCGGC6400.013.24984430
AAGACGG8700.013.2390325
GCGCAAG8250.013.2006361
AGCGAAA8000.013.19944817
ACCGTCG4850.013.19453058
CAAGACG9000.013.1534214
CGTCGTA5000.013.12004310
TCGTTTA5150.013.0483930
TGTAGGA7850.012.8444792
Produced by FastQC (version 0.11.5)