##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062780_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3323551
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26588098091469	32.0	32.0	32.0	32.0	32.0
2	30.89385449478585	32.0	32.0	32.0	32.0	32.0
3	30.942980264181294	32.0	32.0	32.0	32.0	32.0
4	31.007151387175945	32.0	32.0	32.0	32.0	32.0
5	30.919210507075114	32.0	32.0	32.0	32.0	32.0
6	34.57892657582207	36.0	36.0	36.0	32.0	36.0
7	34.520814032942475	36.0	36.0	36.0	32.0	36.0
8	34.49755126369356	36.0	36.0	36.0	32.0	36.0
9	34.637279223336726	36.0	36.0	36.0	32.0	36.0
10	34.37425271945579	36.0	36.0	36.0	32.0	36.0
11	34.625438875467836	36.0	36.0	36.0	32.0	36.0
12	34.46461540683444	36.0	36.0	36.0	32.0	36.0
13	34.53332956226638	36.0	36.0	36.0	32.0	36.0
14	34.43971523229221	36.0	36.0	36.0	32.0	36.0
15	34.391143087619234	36.0	36.0	36.0	32.0	36.0
16	34.405588480513764	36.0	36.0	36.0	32.0	36.0
17	34.34844688707951	36.0	36.0	36.0	32.0	36.0
18	34.36600160490993	36.0	36.0	36.0	32.0	36.0
19	34.35054283806687	36.0	36.0	36.0	32.0	36.0
20	34.33174908403692	36.0	36.0	36.0	32.0	36.0
21	34.31744510615303	36.0	36.0	36.0	32.0	36.0
22	34.29887761614009	36.0	36.0	36.0	32.0	36.0
23	34.245016249186484	36.0	36.0	36.0	32.0	36.0
24	34.237792048324216	36.0	36.0	36.0	32.0	36.0
25	34.216758220349256	36.0	36.0	36.0	32.0	36.0
26	34.16900146860993	36.0	36.0	36.0	32.0	36.0
27	34.18022470544307	36.0	36.0	36.0	32.0	36.0
28	34.160960972165014	36.0	36.0	36.0	32.0	36.0
29	34.12562737866818	36.0	36.0	36.0	32.0	36.0
30	34.11278840011783	36.0	36.0	36.0	32.0	36.0
31	34.122235524594025	36.0	36.0	36.0	32.0	36.0
32	34.078328269973895	36.0	36.0	36.0	32.0	36.0
33	34.04297572084797	36.0	36.0	36.0	32.0	36.0
34	34.04979794202045	36.0	36.0	36.0	32.0	36.0
35	34.0220044765373	36.0	36.0	36.0	32.0	36.0
36	33.985505864059256	36.0	36.0	36.0	32.0	36.0
37	33.984851744414335	36.0	36.0	36.0	32.0	36.0
38	33.402430111648655	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	3.0
7	4.0
8	5.0
9	12.0
10	26.0
11	43.0
12	18.0
13	23.0
14	319.0
15	748.0
16	1059.0
17	1243.0
18	1500.0
19	1979.0
20	2775.0
21	3879.0
22	5862.0
23	9134.0
24	13691.0
25	20544.0
26	30294.0
27	43524.0
28	61152.0
29	82620.0
30	112257.0
31	151067.0
32	206633.0
33	313208.0
34	706202.0
35	1553727.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.92980434129703	16.738818781861827	11.251120991129373	26.08025588571177
2	16.730944821135534	19.212670628621385	37.87153862062924	26.184845929613843
3	19.412317157831733	23.37259223394168	27.88406070981384	29.33102989841274
4	12.852120992926967	15.12613183762619	35.4798768875996	36.54187028184724
5	14.872815097845764	36.10070806881925	33.5761161193147	15.450360714020292
6	34.75118856665225	34.92702512170002	16.712837302142507	13.608949009505222
7	30.73926975673602	30.026387447157404	20.5086428668141	18.725699929292475
8	28.155112828422208	33.18856809993675	18.992598859891043	19.663720211749993
9	27.605802721432305	14.329011440799338	18.101520545538136	39.963665292230225
10	15.650303457194498	26.814116182989604	31.855829323794886	25.679751036021003
11	37.477576201798236	21.211423695297515	22.1634384220386	19.147561680865646
12	25.333824790503844	23.291880425461493	28.637319652770465	22.7369751312642
13	29.821220015630345	19.56300031627828	24.799346859771962	25.816432808319412
14	23.631208729835844	19.41154132252295	25.22150177056978	31.73574817707142
15	25.163878551855625	26.958273831647723	22.319805822613	25.55804179388365
16	25.67471599457868	25.771159878381404	23.613916325828065	24.94020780121185
17	24.35727864796111	25.695853244626697	24.994771075425017	24.952097031987176
18	24.88877187175591	25.03025561556863	25.914694510057622	24.16627800261784
19	25.6242473554757	24.77261935640731	25.40726306103424	24.195870227082743
20	25.89120513507959	24.397736371604807	25.0157296717125	24.695328821603105
21	26.61060034435382	24.1509259097663	24.80674480926638	24.4317289366135
22	25.886327739581034	24.20416990266576	25.096718884984792	24.81278347276841
23	24.696001391427437	24.231149077126123	25.51006467850624	25.5627848529402
24	24.873024346705073	24.73664476127253	25.42666768580214	24.963663206220257
25	25.028821873748306	24.394123738300095	25.382418341033713	25.19463604691789
26	24.70171413369575	25.242115102366814	25.604719728502456	24.451451035434978
27	25.249756863198236	24.78972277590009	24.950861089445457	25.00965927145622
28	24.69672856518271	24.77059996515557	25.516174526673897	25.016496942987832
29	24.644844688573702	25.0294144729438	25.477597756856806	24.84814308162569
30	24.740607857574208	24.909408706573274	25.578353532767075	24.771629903085447
31	25.116750438115048	24.86642907350704	24.885536426136692	25.13128406224122
32	24.94710070965692	24.84828315022195	24.77260526898597	25.432010871135162
33	24.47902765269132	24.68656501739107	25.306137924331463	25.528269405586144
34	25.0871023519591	24.81362293423434	25.313396940569383	24.785877773237175
35	25.42955574654947	24.56626124597047	25.411379224837553	24.592803782642505
36	24.55468647496338	25.251064382982875	25.17583233726105	25.018416804792704
37	25.302007141947946	24.954478883648598	24.9624531661362	24.781060808267256
38	24.652479486683383	25.040433854126388	25.22618133804989	25.08090532114034
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	124.0
1	221.5
2	319.0
3	319.0
4	779.5
5	1240.0
6	1240.0
7	1544.5
8	1849.0
9	1895.0
10	1941.0
11	1941.0
12	2873.5
13	3806.0
14	5204.5
15	6603.0
16	6603.0
17	9897.0
18	13191.0
19	13191.0
20	16805.0
21	20419.0
22	21337.5
23	22256.0
24	22256.0
25	27157.0
26	32058.0
27	32058.0
28	40604.5
29	49151.0
30	61079.0
31	73007.0
32	73007.0
33	90536.0
34	108065.0
35	108065.0
36	122102.0
37	136139.0
38	155658.0
39	175177.0
40	175177.0
41	191707.5
42	208238.0
43	231764.0
44	255290.0
45	255290.0
46	267102.0
47	278914.0
48	278914.0
49	296473.0
50	314032.0
51	317724.0
52	321416.0
53	321416.0
54	310735.5
55	300055.0
56	300055.0
57	290460.5
58	280866.0
59	253612.5
60	226359.0
61	226359.0
62	205131.5
63	183904.0
64	151924.0
65	119944.0
66	119944.0
67	98911.5
68	77879.0
69	77879.0
70	61922.5
71	45966.0
72	35842.5
73	25719.0
74	25719.0
75	19479.0
76	13239.0
77	13239.0
78	11666.0
79	10093.0
80	7797.0
81	5501.0
82	5501.0
83	5275.5
84	5050.0
85	5050.0
86	3411.0
87	1772.0
88	1468.0
89	1164.0
90	1164.0
91	890.0
92	616.0
93	534.5
94	453.0
95	453.0
96	409.5
97	366.0
98	366.0
99	868.0
100	1370.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1186682557300911
2	0.05385805724058394
3	0.011975143453492966
4	0.0025274172112899726
5	1.2035320053761774E-4
6	3.008830013440444E-4
7	3.0088300134404435E-5
8	2.707947012096399E-4
9	2.1061810094083103E-4
10	5.415894024192798E-4
11	0.002196445909811524
12	0.003038918313574848
13	0.01588662247096554
14	0.00983887414395025
15	0.02455205290967402
16	0.011283112550401663
17	0.020700750492470252
18	0.006047748327015292
19	0.008093752736154793
20	0.00643889622876255
21	0.006769867530240998
22	0.007822958034945154
23	0.01038046354636953
24	0.014231765963573298
25	0.016668918274460057
26	0.019497218487094077
27	0.009598167742875016
28	0.006258366427956123
29	0.010741523147982383
30	0.003249536414515679
31	0.00670969092997219
32	0.007672516534273131
33	0.008845960239514904
34	0.013509646760347592
35	0.017541478978357786
36	0.015014061767067814
37	0.011072494449460834
38	0.006679602629837785
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	3323551.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.75790726731175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.29032099334492	37.93353637565058
2	14.965169411804242	15.491317013115482
3	5.210929647102128	8.091204403535922
4	2.3507819229041584	4.866862110853849
5	1.2044213079734134	3.116916318443113
6	0.7286796796716721	2.26289611728125
7	0.4843786397604227	1.7549297303290596
8	0.31112191226193936	1.2882415266945728
9	0.21942921490066314	1.0221477260910792
>10	1.0951464626719252	10.100249392034033
>50	0.07485199275094659	2.676824154277424
>100	0.053968550584036455	5.6834455958762
>500	0.0073558556668340686	2.6118249827129287
>1k	0.0033276489890311156	2.6739494187269908
>5k	1.1675961365021457E-4	0.42565513437755237
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	8958	0.26953099260399493	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	5129	0.15432289138936037	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8052980080642662E-4
2	0.0	0.0	0.0	0.0	1.8052980080642662E-4
3	0.0	0.0	0.0	0.0	1.8052980080642662E-4
4	0.0	0.0	0.0	0.0	1.8052980080642662E-4
5	0.0	0.0	0.0	0.0	2.1061810094083106E-4
6	6.0176600268808876E-5	0.0	0.0	0.0	3.6105960161285323E-4
7	6.0176600268808876E-5	0.0	0.0	0.0	3.6105960161285323E-4
8	6.0176600268808876E-5	0.0	0.0	3.0088300134404438E-5	3.6105960161285323E-4
9	6.0176600268808876E-5	0.0	0.0	3.0088300134404438E-5	3.6105960161285323E-4
10	6.0176600268808876E-5	0.0	0.0	3.0088300134404438E-5	3.911479017472577E-4
11	6.0176600268808876E-5	0.0	0.0	3.0088300134404438E-5	3.911479017472577E-4
12	6.0176600268808876E-5	0.0	0.0	3.0088300134404438E-5	7.522075033601109E-4
13	6.0176600268808876E-5	0.0	0.0	3.0088300134404438E-5	9.929139044353465E-4
14	6.0176600268808876E-5	0.0	0.0	3.0088300134404438E-5	0.0010530905047041552
15	6.0176600268808876E-5	0.0	0.0	3.0088300134404438E-5	0.0013238852059137953
16	6.0176600268808876E-5	0.0	0.0	3.0088300134404438E-5	0.0013539735060481997
17	6.0176600268808876E-5	3.0088300134404438E-5	0.0	1.2035320053761775E-4	0.0013539735060481997
18	6.0176600268808876E-5	3.0088300134404438E-5	0.0	1.5044150067202217E-4	0.0015044150067202219
19	6.0176600268808876E-5	3.0088300134404438E-5	0.0	2.407064010752355E-4	0.0015044150067202219
20	6.0176600268808876E-5	3.0088300134404438E-5	0.0	3.6105960161285323E-4	0.0016849448075266485
21	6.0176600268808876E-5	3.0088300134404438E-5	0.0	4.5132450201606654E-4	0.0017752097079298617
22	9.026490040321331E-5	3.0088300134404438E-5	3.0088300134404438E-5	5.415894024192798E-4	0.0018353863081986706
23	9.026490040321331E-5	3.0088300134404438E-5	3.0088300134404438E-5	7.221192032257065E-4	0.0019557395087362885
24	9.026490040321331E-5	3.0088300134404438E-5	3.0088300134404438E-5	0.0012637086056449863	0.001985827808870693
25	9.026490040321331E-5	3.0088300134404438E-5	3.0088300134404438E-5	0.0014743267065858174	0.0020159161090050975
26	9.026490040321331E-5	3.0088300134404438E-5	3.0088300134404438E-5	0.0016247682072578395	0.0021663576096771194
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5770	0.0	16.653873	1
TTACGCT	60	0.002445857	15.997405	4
TCTAGCG	555	0.0	14.989913	28
TCAATCG	65	0.004164106	14.769057	25
CTAGCGG	590	0.0	14.643895	29
GTATTAC	255	0.0	14.445332	1
AACGCAG	6770	0.0	14.012287	6
GTCCTAT	895	0.0	13.420789	1
GTATTAG	575	0.0	13.36944	1
ATACCGT	530	0.0	13.280664	6
GTATAGG	290	0.0	13.254187	1
TAGCGGC	640	0.0	13.249844	30
AAGACGG	870	0.0	13.239032	5
GCGCAAG	825	0.0	13.200636	1
AGCGAAA	800	0.0	13.199448	17
ACCGTCG	485	0.0	13.1945305	8
CAAGACG	900	0.0	13.153421	4
CGTCGTA	500	0.0	13.120043	10
TCGTTTA	515	0.0	13.04839	30
TGTAGGA	785	0.0	12.844479	2
>>END_MODULE