FastQCFastQC Report
Thu 2 Feb 2017
SRR4062780_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062780_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3323551
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT89470.2692000213025165No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT57260.1722856065695998No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT37190.1118983881998501No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACG400.004486026719.9993920
CTAGCGG5400.016.59208729
GTATCAA54050.016.0155181
TCTAGCG5650.015.85792228
ACCGTCG5300.015.6995228
TACCGTC5400.015.4087917
GTCCTAA5250.015.2387751
CGTTTAT5050.015.20745631
GCGGATA756.250204E-414.93287821
TCGTTTA5150.014.91216730
ATACCGT5600.014.8584786
ATCGTTT5450.014.38488129
CGTCGTA5850.014.22307110
AAGACGG8200.014.0502545
GTTACGT800.001018545214.0002043
AACCGCG3300.013.5769777
TAGCGGC6600.013.57534230
CAAGACG9250.013.4922924
CATCGTT5700.013.47327228
GCGCAAG8200.013.4640161
Produced by FastQC (version 0.11.5)