##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062780_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3323551
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.17735819308926	32.0	32.0	32.0	32.0	32.0
2	31.234324371733727	32.0	32.0	32.0	32.0	32.0
3	31.344979210489022	32.0	32.0	32.0	32.0	32.0
4	31.45297725234245	32.0	32.0	32.0	32.0	32.0
5	31.362660900946008	32.0	32.0	32.0	32.0	32.0
6	34.885605486421	36.0	36.0	36.0	36.0	36.0
7	34.926839997340196	36.0	36.0	36.0	36.0	36.0
8	34.85370647238451	36.0	36.0	36.0	32.0	36.0
9	34.96914805880819	36.0	36.0	36.0	36.0	36.0
10	34.81182115153341	36.0	36.0	36.0	32.0	36.0
11	34.997083841950975	36.0	36.0	36.0	36.0	36.0
12	34.90248652721141	36.0	36.0	36.0	32.0	36.0
13	34.95584963191478	36.0	36.0	36.0	36.0	36.0
14	34.902621021913006	36.0	36.0	36.0	32.0	36.0
15	34.865105725773425	36.0	36.0	36.0	32.0	36.0
16	34.88411521291534	36.0	36.0	36.0	32.0	36.0
17	34.8554293284502	36.0	36.0	36.0	32.0	36.0
18	34.85335293485793	36.0	36.0	36.0	32.0	36.0
19	34.84666761545106	36.0	36.0	36.0	32.0	36.0
20	34.83247376074566	36.0	36.0	36.0	32.0	36.0
21	34.83060888790333	36.0	36.0	36.0	32.0	36.0
22	34.81482998154684	36.0	36.0	36.0	32.0	36.0
23	34.75763242387434	36.0	36.0	36.0	32.0	36.0
24	34.740784781097084	36.0	36.0	36.0	32.0	36.0
25	34.72637970652474	36.0	36.0	36.0	32.0	36.0
26	34.66069183232031	36.0	36.0	36.0	32.0	36.0
27	34.646679710947716	36.0	36.0	36.0	32.0	36.0
28	34.61851164612789	36.0	36.0	36.0	32.0	36.0
29	34.591225469385	36.0	36.0	36.0	32.0	36.0
30	34.56608218137769	36.0	36.0	36.0	32.0	36.0
31	34.561209080287924	36.0	36.0	36.0	32.0	36.0
32	34.53835791898484	36.0	36.0	36.0	32.0	36.0
33	34.507776170728235	36.0	36.0	36.0	32.0	36.0
34	34.502342524606966	36.0	36.0	36.0	32.0	36.0
35	34.47532443461827	36.0	36.0	36.0	32.0	36.0
36	34.45211823137361	36.0	36.0	36.0	32.0	36.0
37	34.4479744706791	36.0	36.0	36.0	32.0	36.0
38	34.0296622498045	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	3.0
20	15.0
21	83.0
22	267.0
23	834.0
24	2299.0
25	5528.0
26	11503.0
27	22390.0
28	38301.0
29	60733.0
30	91621.0
31	132373.0
32	192256.0
33	310285.0
34	758222.0
35	1696837.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.298037807908045	17.927747735440335	11.921891460617289	26.852322996034335
2	15.82656622389727	20.161838948762934	37.08629715626449	26.925297671075306
3	18.305902331572465	24.11820971003604	28.311164775265972	29.26472318312552
4	12.408561463305436	16.06757726089981	35.469968202617444	36.053893073177306
5	14.43598828721595	36.68251519159645	33.171387926396726	15.710108594790867
6	34.82031852706806	35.39401266357519	16.33525377097006	13.450415038386687
7	30.466881698429987	30.199486090132808	20.544028355307894	18.789603856129308
8	28.05187599444435	33.45858417602838	18.79803722862652	19.691502600900748
9	27.471814248003323	14.155838645916424	17.845675466593086	40.52667163948717
10	15.414986344293503	27.120169916284514	31.790701362020197	25.674142377401783
11	37.60583383733981	21.103036977525825	22.04828231584205	19.242846869292315
12	25.18404316360171	23.443851001085893	28.557914585678347	22.81419124963405
13	29.824320168879865	19.56124572971158	24.773690510075685	25.84074359133287
14	23.539040020748892	19.363445904696512	25.20716546850041	31.890348606054186
15	25.190075314024067	27.088436434404045	22.098382122013476	25.623106129558415
16	25.652201515788388	25.81895087513325	23.51882068305857	25.01002692601979
17	24.283304212873517	25.816603987722768	24.8342510766346	25.06584072276911
18	24.899362158125452	25.045862091479865	25.840133038427876	24.214642711966807
19	25.633035268602768	24.825886529197234	25.41029158270777	24.130786619492223
20	25.97288261862087	24.336470239211014	24.95394233456926	24.736704807598862
21	26.58554440246448	24.233897379994783	24.726925452747263	24.453632764793475
22	25.81737949925802	24.36068322166552	24.99705134392523	24.82488593515123
23	24.714446557983553	24.281475267680968	25.396820143304772	25.60725803103071
24	24.962246705406358	24.780784167295764	25.219291053454572	25.037678073843306
25	25.000782296509634	24.449654405472707	25.404387118826026	25.145176179191637
26	24.821523725677746	25.21270171572514	25.470799154278062	24.494975404319057
27	25.318815916003018	24.82061339267554	24.839057537306516	25.021513154014922
28	24.69488207041204	24.77359306356364	25.433429485511127	25.09809538051319
29	24.757225028290524	24.936160149189828	25.38480679249393	24.921808030025716
30	24.72605354935128	24.899422334725717	25.565396769900627	24.809127346022372
31	25.125505821935633	24.83747052474898	24.884648979359728	25.152374673955656
32	24.988754497824768	24.783612467508398	24.75734538149106	25.470287653175777
33	24.483541850267983	24.67776182763556	25.27125354778669	25.567442774309768
34	25.044357676473144	24.747747213748188	25.386732443702538	24.821162666076134
35	25.423620699667314	24.559695337908156	25.392268690927267	24.624415271497263
36	24.692264388300345	25.157610038179044	25.083081318746125	25.067044254774483
37	25.31226390086988	24.884859597460668	24.969317455937944	24.83355904573151
38	24.70929436617642	24.922620414129344	25.163356903504713	25.204728316189524
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	60.0
1	120.5
2	181.0
3	181.0
4	553.5
5	926.0
6	926.0
7	1258.0
8	1590.0
9	1708.5
10	1827.0
11	1827.0
12	2724.0
13	3621.0
14	5061.5
15	6502.0
16	6502.0
17	10172.5
18	13843.0
19	13843.0
20	16947.0
21	20051.0
22	21517.5
23	22984.0
24	22984.0
25	27490.5
26	31997.0
27	31997.0
28	40667.0
29	49337.0
30	61071.0
31	72805.0
32	72805.0
33	90731.0
34	108657.0
35	108657.0
36	122783.5
37	136910.0
38	156174.0
39	175438.0
40	175438.0
41	192348.5
42	209259.0
43	233046.0
44	256833.0
45	256833.0
46	267481.5
47	278130.0
48	278130.0
49	296768.5
50	315407.0
51	318127.0
52	320847.0
53	320847.0
54	310448.5
55	300050.0
56	300050.0
57	290510.5
58	280971.0
59	253963.0
60	226955.0
61	226955.0
62	206110.5
63	185266.0
64	152329.0
65	119392.0
66	119392.0
67	98603.5
68	77815.0
69	77815.0
70	61880.0
71	45945.0
72	35668.0
73	25391.0
74	25391.0
75	19229.0
76	13067.0
77	13067.0
78	11457.0
79	9847.0
80	7532.5
81	5218.0
82	5218.0
83	4675.5
84	4133.0
85	4133.0
86	2700.5
87	1268.0
88	1006.5
89	745.0
90	745.0
91	443.5
92	142.0
93	113.0
94	84.0
95	84.0
96	58.0
97	32.0
98	32.0
99	28.5
100	25.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008364547437364433
2	0.0
3	0.0
4	2.1061810094083103E-4
5	9.026490040321331E-5
6	9.026490040321331E-5
7	3.3097130147844876E-4
8	0.002617682111693186
9	0.0045734216204294745
10	9.628256043009419E-4
11	0.0054459823243272025
12	3.008830013440444E-4
13	1.504415006720222E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	2.4070640107523548E-4
22	9.026490040321331E-5
23	1.504415006720222E-4
24	0.0
25	9.026490040321331E-5
26	0.0
27	9.026490040321331E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	3323551.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.75749110496825
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.30473505025903	35.47944720005381
2	15.927490763665775	15.850239599951276
3	5.641558664766843	8.421294152408782
4	2.5339486936091187	5.043317183308066
5	1.3625285763417856	3.3898001758795733
6	0.7932572245990297	2.368229357816281
7	0.5026612021323276	1.7507812205738358
8	0.3387580071015188	1.3484598820072475
9	0.2533734692767568	1.1346505329395855
>10	1.1899787035604805	10.446968157270868
>50	0.08313720655664068	2.8796260633050097
>100	0.05687927394964489	5.832232313733772
>500	0.007795442789207154	2.6714441280257875
>1k	0.003775917598372449	2.9388703595346835
>5k	1.2180379349588547E-4	0.4446396731914418
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	8947	0.2692000213025165	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	5726	0.1722856065695998	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	3719	0.1118983881998501	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.026490040321331E-5
2	0.0	0.0	0.0	0.0	9.026490040321331E-5
3	0.0	0.0	0.0	0.0	9.026490040321331E-5
4	0.0	0.0	0.0	0.0	1.2035320053761775E-4
5	0.0	0.0	0.0	0.0	1.2035320053761775E-4
6	3.0088300134404438E-5	0.0	0.0	0.0	1.8052980080642662E-4
7	3.0088300134404438E-5	0.0	0.0	0.0	1.8052980080642662E-4
8	3.0088300134404438E-5	0.0	0.0	0.0	2.407064010752355E-4
9	3.0088300134404438E-5	0.0	0.0	0.0	2.407064010752355E-4
10	3.0088300134404438E-5	0.0	0.0	0.0	2.407064010752355E-4
11	6.0176600268808876E-5	0.0	0.0	0.0	2.707947012096399E-4
12	6.0176600268808876E-5	0.0	0.0	0.0	6.017660026880887E-4
13	6.0176600268808876E-5	0.0	0.0	0.0	7.522075033601109E-4
14	6.0176600268808876E-5	0.0	0.0	0.0	7.522075033601109E-4
15	6.0176600268808876E-5	3.0088300134404438E-5	0.0	3.0088300134404438E-5	0.0010530905047041552
16	1.5044150067202217E-4	3.0088300134404438E-5	0.0	3.0088300134404438E-5	0.0010831788048385597
17	1.5044150067202217E-4	3.0088300134404438E-5	0.0	9.026490040321331E-5	0.0010831788048385597
18	1.5044150067202217E-4	3.0088300134404438E-5	0.0	1.2035320053761775E-4	0.0012035320053761774
19	1.5044150067202217E-4	3.0088300134404438E-5	0.0	1.5044150067202217E-4	0.0012035320053761774
20	1.5044150067202217E-4	3.0088300134404438E-5	0.0	2.707947012096399E-4	0.0013840618061826042
21	1.5044150067202217E-4	3.0088300134404438E-5	0.0	3.911479017472577E-4	0.0014743267065858174
22	1.5044150067202217E-4	3.0088300134404438E-5	0.0	4.5132450201606654E-4	0.0015345033068546263
23	2.1061810094083106E-4	3.0088300134404438E-5	0.0	6.318543028224932E-4	0.001715033107661053
24	2.1061810094083106E-4	3.0088300134404438E-5	0.0	0.0011132671049729642	0.0017451214077954574
25	2.1061810094083106E-4	3.0088300134404438E-5	0.0	0.0012937969057793908	0.0017451214077954574
26	2.1061810094083106E-4	3.0088300134404438E-5	0.0	0.0015345033068546263	0.0018353863081986706
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATACG	40	0.0044860267	19.99939	20
CTAGCGG	540	0.0	16.592087	29
GTATCAA	5405	0.0	16.015518	1
TCTAGCG	565	0.0	15.857922	28
ACCGTCG	530	0.0	15.699522	8
TACCGTC	540	0.0	15.408791	7
GTCCTAA	525	0.0	15.238775	1
CGTTTAT	505	0.0	15.207456	31
GCGGATA	75	6.250204E-4	14.932878	21
TCGTTTA	515	0.0	14.912167	30
ATACCGT	560	0.0	14.858478	6
ATCGTTT	545	0.0	14.384881	29
CGTCGTA	585	0.0	14.223071	10
AAGACGG	820	0.0	14.050254	5
GTTACGT	80	0.0010185452	14.000204	3
AACCGCG	330	0.0	13.576977	7
TAGCGGC	660	0.0	13.575342	30
CAAGACG	925	0.0	13.492292	4
CATCGTT	570	0.0	13.473272	28
GCGCAAG	820	0.0	13.464016	1
>>END_MODULE