FastQCFastQC Report
Thu 2 Feb 2017
SRR4062779_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062779_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3407753
Sequences flagged as poor quality0
Sequence length38
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT202340.593763691206493No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT125360.36786703731168313No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT68180.20007318605544475No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT61350.1800306536301193No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA53920.15822743021574628No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA53610.15731773987140502No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG48190.1414128312703415No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC45660.13398858426652402No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA44460.13046720228842879No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG6650.017.56357229
TCTAGCG6700.017.43198828
TAGCGGC6800.017.17639230
TGTAGGA45350.016.1631982
GTCCTAC41250.015.9165051
ATCGTTT5450.015.55933429
GATATAC12600.015.5051921
GTAGGAC45150.015.3068783
CTGTAGG45650.015.2944391
AGGACCT28750.014.96641355
TCCTACA45550.014.8273382
CATCGTT5850.014.76851428
TAACGCA650.004170033614.7659134
TTAGGAC29000.014.6738563
TAGGACC21500.014.5083684
CGGTCCA8300.014.45797710
ATACCGT6750.014.4553746
TTTAGAA18400.014.4387432
CCTACAG45500.014.3452933
GATAACG4250.014.30602711
Produced by FastQC (version 0.11.5)