##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062779_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3407753
Sequences flagged as poor quality	0
Sequence length	38
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.164776907246505	32.0	32.0	32.0	32.0	32.0
2	30.873846490634737	32.0	32.0	32.0	32.0	32.0
3	30.934872920660624	32.0	32.0	32.0	32.0	32.0
4	30.965712303679286	32.0	32.0	32.0	32.0	32.0
5	30.951588627462144	32.0	32.0	32.0	32.0	32.0
6	34.578155752485586	36.0	36.0	36.0	32.0	36.0
7	34.47277252782112	36.0	36.0	36.0	32.0	36.0
8	34.46089696054849	36.0	36.0	36.0	32.0	36.0
9	34.539743637523024	36.0	36.0	36.0	32.0	36.0
10	34.341441119705564	36.0	36.0	36.0	32.0	36.0
11	34.570769360338026	36.0	36.0	36.0	32.0	36.0
12	34.41914540167671	36.0	36.0	36.0	32.0	36.0
13	34.48338773379409	36.0	36.0	36.0	32.0	36.0
14	34.41199611591568	36.0	36.0	36.0	32.0	36.0
15	34.37957533894035	36.0	36.0	36.0	32.0	36.0
16	34.3952742466957	36.0	36.0	36.0	32.0	36.0
17	34.334685935277584	36.0	36.0	36.0	32.0	36.0
18	34.3612092777851	36.0	36.0	36.0	32.0	36.0
19	34.3270803371019	36.0	36.0	36.0	32.0	36.0
20	34.2885611134375	36.0	36.0	36.0	32.0	36.0
21	34.261398053203976	36.0	36.0	36.0	32.0	36.0
22	34.242982399252526	36.0	36.0	36.0	32.0	36.0
23	34.2198539624204	36.0	36.0	36.0	32.0	36.0
24	34.202683997343705	36.0	36.0	36.0	32.0	36.0
25	34.18016740063027	36.0	36.0	36.0	32.0	36.0
26	34.14921944166728	36.0	36.0	36.0	32.0	36.0
27	34.1447311468877	36.0	36.0	36.0	32.0	36.0
28	34.11204773350651	36.0	36.0	36.0	32.0	36.0
29	34.071550960412914	36.0	36.0	36.0	32.0	36.0
30	34.03667255226538	36.0	36.0	36.0	32.0	36.0
31	34.040939586877336	36.0	36.0	36.0	32.0	36.0
32	33.99594424830673	36.0	36.0	36.0	32.0	36.0
33	33.96882960707539	36.0	36.0	36.0	32.0	36.0
34	33.947267011429524	36.0	36.0	36.0	32.0	36.0
35	33.91276832563862	36.0	36.0	36.0	32.0	36.0
36	33.86267109147875	36.0	36.0	36.0	32.0	36.0
37	33.83578856800948	36.0	36.0	36.0	32.0	36.0
38	33.20681428495551	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	2.0
8	5.0
9	12.0
10	31.0
11	44.0
12	18.0
13	17.0
14	879.0
15	1687.0
16	1996.0
17	2465.0
18	3078.0
19	3832.0
20	4730.0
21	6118.0
22	8474.0
23	11616.0
24	15584.0
25	21822.0
26	31325.0
27	44125.0
28	61423.0
29	83799.0
30	112802.0
31	151377.0
32	206436.0
33	312055.0
34	715006.0
35	1606994.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.96248572472001	18.625542108072867	12.608961482378566	24.803010684828557
2	14.546443222750597	20.779127356690104	41.322835274511164	23.351594146048136
3	18.54373790275835	26.239542627047634	29.1638944203376	26.05282504985641
4	11.856667411075215	16.69067653237869	37.72091252945231	33.73174352709378
5	13.029055871257972	38.18293328872906	34.22756104109902	14.560449798913943
6	31.14673520076626	37.3560044416482	18.287641325169965	13.209619032415581
7	27.875737436292315	31.903759428503015	22.076782582770107	18.143720552434566
8	26.1646184414512	35.37797847081958	20.350747869486792	18.106655218242434
9	27.455698015228826	14.932060704019193	20.00326902106611	37.60897225968587
10	15.805006422139229	27.762745019210662	32.64462725035309	23.787621308297016
11	34.94403108259395	21.986914908757974	24.71923103446758	18.349822974180494
12	25.275783331479662	24.439314168063277	30.33444974068693	19.95045275977013
13	29.157477916182163	21.447761623316897	26.097557969104667	23.297202491396277
14	22.336688069950853	22.089169492117353	26.880769943015554	28.693372494916243
15	22.98487284258886	30.639178683144507	23.899188680447057	22.47675979381958
16	22.61291489880603	27.188970251810268	27.582529266732937	22.61558558265077
17	21.49387134296265	27.848267712583436	27.690622939002246	22.967238005451673
18	22.157337122552722	25.88710051350496	30.719822559354753	21.23573980458756
19	24.335515096306988	25.502268679370644	27.654762788669313	22.50745343565306
20	24.62886868410596	25.74447829733586	27.945118404579944	21.681534613978236
21	25.01315470134206	25.263893727034297	26.50159485163287	23.221356719990773
22	24.244012540583306	26.962576929634864	27.001109612128786	21.792300917653044
23	21.854800439160922	26.860571689850964	28.22644902707543	23.05817884391269
24	23.521112829999407	26.399277303836154	28.243349971428493	21.836259894735946
25	23.004929910855402	26.42651756110146	28.29120784136705	22.27734468667609
26	21.47479039199031	27.839841459269742	28.9718978807035	21.713470268036446
27	22.52552865856969	27.242640746952627	27.738848315966948	22.49298227851073
28	21.541537064639165	27.50571011933218	27.742449333787615	23.210303482241038
29	22.51548265197534	26.512592439547074	27.58298061667648	23.388944291801106
30	21.792548591390375	26.98413071056866	28.298261764482334	22.92505893355863
31	23.333201273061025	26.233949174403197	27.348596566139456	23.084252986396326
32	22.18307988802371	27.5584760354124	27.27064097006991	22.987803106493985
33	21.387874935362298	27.231475465259397	28.784453143021153	22.596196456357152
34	22.372927617387322	27.280904878041625	28.61597565234745	21.7301918522236
35	22.62144416663267	27.382679474510173	28.218208414399815	21.77766794445734
36	21.491550912881777	27.730368815507724	28.200545193282995	22.57753507832751
37	22.45425499226541	27.659012490678435	26.97177718867143	22.91495532838472
38	22.112198242720556	28.325360817481233	27.222453734952488	22.339987204845723
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2967.0
1	2252.5
2	1538.0
3	1538.0
4	3402.5
5	5267.0
6	5267.0
7	6091.0
8	6915.0
9	6473.0
10	6031.0
11	6031.0
12	8135.0
13	10239.0
14	13109.5
15	15980.0
16	15980.0
17	22864.5
18	29749.0
19	29749.0
20	36246.0
21	42743.0
22	43808.5
23	44874.0
24	44874.0
25	54172.5
26	63471.0
27	63471.0
28	82167.0
29	100863.0
30	126555.5
31	152248.0
32	152248.0
33	181086.0
34	209924.0
35	209924.0
36	221970.5
37	234017.0
38	247428.0
39	260839.0
40	260839.0
41	254426.0
42	248013.0
43	254421.5
44	260830.0
45	260830.0
46	261661.5
47	262493.0
48	262493.0
49	266374.0
50	270255.0
51	261132.0
52	252009.0
53	252009.0
54	236142.5
55	220276.0
56	220276.0
57	210364.5
58	200453.0
59	175953.0
60	151453.0
61	151453.0
62	140283.0
63	129113.0
64	105623.0
65	82133.0
66	82133.0
67	69629.0
68	57125.0
69	57125.0
70	44874.0
71	32623.0
72	25654.5
73	18686.0
74	18686.0
75	13971.0
76	9256.0
77	9256.0
78	9153.0
79	9050.0
80	7029.0
81	5008.0
82	5008.0
83	5055.0
84	5102.0
85	5102.0
86	3430.5
87	1759.0
88	1557.5
89	1356.0
90	1356.0
91	973.0
92	590.0
93	543.5
94	497.0
95	497.0
96	451.0
97	405.0
98	405.0
99	1004.0
100	1603.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12154636794392082
2	0.05625407710007151
3	0.01373338971457145
4	0.0036974510770000054
5	2.3475879853968292E-4
6	2.6410364835714327E-4
7	2.9344849817460365E-5
8	1.7606909890476217E-4
9	1.4672424908730182E-4
10	2.9344849817460365E-4
11	0.001702001289412701
12	0.0028171055824761948
13	0.014877838857452403
14	0.00974249013939684
15	0.02447360474776194
16	0.011327112029539699
17	0.021304360967476222
18	0.0056048663151349295
19	0.007747040351809535
20	0.005839625113674612
21	0.006631936058746042
22	0.0075122815532698535
23	0.010153318036841285
24	0.015024563106539707
25	0.017372151091936537
26	0.019925153026055586
27	0.009038213743777793
28	0.0060450390623968345
29	0.011473836278627001
30	0.002993174681380957
31	0.006397177260206359
32	0.007218833055095249
33	0.008774110095420649
34	0.012999768469134941
35	0.016726564395952406
36	0.01528866675489685
37	0.00974249013939684
38	0.0062504530111190575
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	3407753.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.09963119629187
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.35210526429616	32.179536324987524
2	15.293208545705141	13.79436130438562
3	5.8886571640106595	7.967287990148482
4	2.739108117948141	4.941310637049211
5	1.4637645044172944	3.3007619653721463
6	0.8295021107262059	2.2446143562178533
7	0.524680689481957	1.6564033914016678
8	0.35220454824985725	1.2707436185380117
9	0.24801497175445703	1.0066845381556282
>10	1.1085169026048236	8.706410970275865
>50	0.08977110549046931	2.8727829925340775
>100	0.08903317158697746	8.588286885731968
>500	0.01385297436163619	4.294515988195789
>1k	0.00718786405420416	5.511951224931437
>5k	2.613768746983331E-4	0.6986158586487341
>10k+	1.3068843734916655E-4	0.9657319534260954
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	20234	0.593763691206493	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	12536	0.36786703731168313	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	6818	0.20007318605544475	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6135	0.1800306536301193	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	5392	0.15822743021574628	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA	5361	0.15731773987140502	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG	4819	0.1414128312703415	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	4566	0.13398858426652402	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	4446	0.13046720228842879	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.9344849817460362E-5	0.0
5	2.9344849817460362E-5	0.0	0.0	5.8689699634920724E-5	0.0
6	2.9344849817460362E-5	0.0	0.0	5.8689699634920724E-5	0.0
7	2.9344849817460362E-5	0.0	0.0	5.8689699634920724E-5	0.0
8	2.9344849817460362E-5	0.0	0.0	8.803454945238109E-5	0.0
9	2.9344849817460362E-5	0.0	0.0	8.803454945238109E-5	0.0
10	2.9344849817460362E-5	0.0	0.0	8.803454945238109E-5	0.0
11	2.9344849817460362E-5	0.0	0.0	1.1737939926984145E-4	0.0
12	2.9344849817460362E-5	0.0	0.0	1.1737939926984145E-4	0.0
13	2.9344849817460362E-5	0.0	0.0	1.467242490873018E-4	0.0
14	2.9344849817460362E-5	0.0	0.0	1.467242490873018E-4	0.0
15	2.9344849817460362E-5	0.0	0.0	1.467242490873018E-4	0.0
16	2.9344849817460362E-5	0.0	0.0	2.0541394872222252E-4	0.0
17	2.9344849817460362E-5	0.0	0.0	2.347587985396829E-4	0.0
18	2.9344849817460362E-5	0.0	0.0	2.934484981746036E-4	0.0
19	2.9344849817460362E-5	0.0	0.0	2.934484981746036E-4	0.0
20	2.9344849817460362E-5	0.0	0.0	5.575521465317469E-4	0.0
21	2.9344849817460362E-5	0.0	0.0	9.977248937936523E-4	0.0
22	2.9344849817460362E-5	0.0	0.0	0.0015846218901428596	0.0
23	2.9344849817460362E-5	0.0	0.0	0.0034333474286428623	0.0
24	2.9344849817460362E-5	0.0	0.0	0.0061037287620317555	0.0
25	2.9344849817460362E-5	0.0	0.0	0.007072108806007948	0.0
26	2.9344849817460362E-5	0.0	0.0	0.009361007091769855	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	665	0.0	17.563572	29
TCTAGCG	670	0.0	17.431988	28
TAGCGGC	680	0.0	17.176392	30
TGTAGGA	4535	0.0	16.163198	2
GTCCTAC	4125	0.0	15.916505	1
ATCGTTT	545	0.0	15.559334	29
GATATAC	1260	0.0	15.505192	1
GTAGGAC	4515	0.0	15.306878	3
CTGTAGG	4565	0.0	15.294439	1
AGGACCT	2875	0.0	14.9664135	5
TCCTACA	4555	0.0	14.827338	2
CATCGTT	585	0.0	14.768514	28
TAACGCA	65	0.0041700336	14.765913	4
TTAGGAC	2900	0.0	14.673856	3
TAGGACC	2150	0.0	14.508368	4
CGGTCCA	830	0.0	14.457977	10
ATACCGT	675	0.0	14.455374	6
TTTAGAA	1840	0.0	14.438743	2
CCTACAG	4550	0.0	14.345293	3
GATAACG	425	0.0	14.306027	11
>>END_MODULE