FastQCFastQC Report
Thu 2 Feb 2017
SRR4062779_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062779_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3407753
Sequences flagged as poor quality0
Sequence length38
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT227150.6665682636036121No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT156230.45845458869818323No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT86510.25386229577084957No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT70290.20626494936692888No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA55440.16268784738800024No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG49280.14461141990044468No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA47730.1400629681787383No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC41750.12251474798789701No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA39150.11488508703535731No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTA350.002067572722.85675419
CTAGCGG5900.017.89800329
TATATCG450.00885161417.778525
TAGCGGC6000.017.3330430
TCTAGCG6150.016.91028228
ACCGTCG5250.016.7626048
TAAGACC2950.015.7285474
TACCGTC5750.015.5832597
AGGACCT31500.015.5435055
GACGCGT951.4334699E-515.1585278
TGTAGGA44600.014.887642
AGCGGCG6900.014.84032831
TAGACCG650.004162656614.7698465
GGACCGA1951.8189894E-1214.7698466
TCCTCGC14200.014.64763829
CGGTCCA7250.014.56612410
ATACCGT6050.014.5460616
GGACCTG33100.014.5021166
CCGTCGT5950.014.2526969
ACCGTCC1351.3204044E-714.2228168
Produced by FastQC (version 0.11.5)