##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062779_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3407753
Sequences flagged as poor quality	0
Sequence length	38
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.116504629296784	32.0	32.0	32.0	32.0	32.0
2	31.291138178148476	32.0	32.0	32.0	32.0	32.0
3	31.375977073455736	32.0	32.0	32.0	32.0	32.0
4	31.47589599363569	32.0	32.0	32.0	32.0	32.0
5	31.398044693966963	32.0	32.0	32.0	32.0	32.0
6	34.905556535347486	36.0	36.0	36.0	36.0	36.0
7	34.937494882991814	36.0	36.0	36.0	36.0	36.0
8	34.88353586659596	36.0	36.0	36.0	32.0	36.0
9	34.98706713778845	36.0	36.0	36.0	36.0	36.0
10	34.84414069916452	36.0	36.0	36.0	32.0	36.0
11	35.0158670537448	36.0	36.0	36.0	36.0	36.0
12	34.91676392038977	36.0	36.0	36.0	32.0	36.0
13	34.96602423943285	36.0	36.0	36.0	36.0	36.0
14	34.91709786478069	36.0	36.0	36.0	32.0	36.0
15	34.884085495633045	36.0	36.0	36.0	32.0	36.0
16	34.89848237240199	36.0	36.0	36.0	32.0	36.0
17	34.87638012496798	36.0	36.0	36.0	32.0	36.0
18	34.87334850853333	36.0	36.0	36.0	32.0	36.0
19	34.85110335168071	36.0	36.0	36.0	32.0	36.0
20	34.83629740770531	36.0	36.0	36.0	32.0	36.0
21	34.819873975607976	36.0	36.0	36.0	32.0	36.0
22	34.803185852965285	36.0	36.0	36.0	32.0	36.0
23	34.76709873045376	36.0	36.0	36.0	32.0	36.0
24	34.74462321653007	36.0	36.0	36.0	32.0	36.0
25	34.722361920010044	36.0	36.0	36.0	32.0	36.0
26	34.6608466047862	36.0	36.0	36.0	32.0	36.0
27	34.63578052752063	36.0	36.0	36.0	32.0	36.0
28	34.596034689133866	36.0	36.0	36.0	32.0	36.0
29	34.55703186234448	36.0	36.0	36.0	32.0	36.0
30	34.52677673528569	36.0	36.0	36.0	32.0	36.0
31	34.51603094473103	36.0	36.0	36.0	32.0	36.0
32	34.476803629840546	36.0	36.0	36.0	32.0	36.0
33	34.44223686399807	36.0	36.0	36.0	32.0	36.0
34	34.41416147238371	36.0	36.0	36.0	32.0	36.0
35	34.384863574325955	36.0	36.0	36.0	32.0	36.0
36	34.34448388718314	36.0	36.0	36.0	32.0	36.0
37	34.311970380482386	36.0	36.0	36.0	32.0	36.0
38	33.89037732488241	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	6.0
20	28.0
21	91.0
22	251.0
23	831.0
24	2195.0
25	5586.0
26	11952.0
27	23317.0
28	40350.0
29	64020.0
30	97312.0
31	139313.0
32	200399.0
33	317895.0
34	764806.0
35	1739399.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.31115903256063	19.891660219966496	13.339709599146335	25.457471148326537
2	13.765669049370654	21.870188361656492	40.37378882800485	23.990353760968006
3	17.702075238434244	26.842731852924786	29.60936429371495	25.845828614926024
4	11.42892272875731	17.60690121250127	37.70500702956702	33.25916902917439
5	12.637451316879867	38.71971904058746	33.8895553432292	14.75327429930347
6	31.23027474718663	37.765963534307524	17.794199165373293	13.209562553132551
7	27.641785193308298	32.09772567943064	22.11733690011248	18.143152227148583
8	26.01324831358822	35.666798427903565	20.14397482861502	18.175978429893192
9	27.1807276859184	14.735898092043017	19.941460677586495	38.14191354445209
10	15.649240928973581	28.079670911736244	32.51645235902736	23.754635800262815
11	34.958355868989074	21.87536958230853	24.737385633075604	18.4288889156268
12	25.086971104904983	24.60941133730527	30.293117933551876	20.010499624237873
13	29.24595905915523	21.406877155750458	26.07380362268784	23.27336016240647
14	22.203464336606654	22.131158605438426	26.886140775502444	28.77923628245248
15	22.96052560147405	30.692365321078142	23.84691613506026	22.50019294238755
16	22.67981004469519	27.22694658556132	27.33294030751678	22.760303062226708
17	21.501939841297183	27.94697855155582	27.58439358721128	22.966688019935717
18	22.24644802601597	25.814605694720242	30.730396246441572	21.208550032822217
19	24.433842476259283	25.4187436706827	27.759758409720426	22.38765544333759
20	24.677874247341283	25.740216500432982	27.971364121754128	21.610545130471603
21	24.907754383390802	25.24642359328002	26.690983786956203	23.154838236372974
22	24.182188140451537	26.908002695031797	27.17741784026537	21.732391324251296
23	21.767552852306405	26.759877702332126	28.330825814444776	23.141743630916693
24	23.514028158730987	26.36708118223357	28.23778601324685	21.88110464578859
25	22.96133812632969	26.345770669796785	28.322764287286333	22.37012691658719
26	21.53528191018595	27.65591510180318	29.014317943324514	21.79448504468635
27	22.651541517472253	27.099731024554057	27.83088880450597	22.41783865346772
28	21.603179573167424	27.27597921562977	28.02048740034856	23.100353810854248
29	22.647239984822846	26.19042518633246	27.757674925383384	23.404659903461315
30	21.852581451766017	26.60650581189423	28.53302454726032	23.00788818907943
31	23.30465265528341	25.918691877022777	27.632343071813008	23.144312395880803
32	22.28852854065421	27.274218524640727	27.535299653466666	22.9019532812384
33	21.47737820199997	26.996704279917	28.90948962556852	22.61642789251451
34	22.47026119557374	27.01208098122135	28.721682586736776	21.795975236468134
35	22.754495410905662	27.14448494359773	28.25269319695412	21.848326448542483
36	21.61999417211283	27.435761922885842	28.35994862303694	22.58429528196439
37	22.591983632616568	27.334434156466152	27.054073461310136	23.019508749607144
38	22.28581254909763	27.79143781338484	27.462922026873443	22.459827610644087
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1754.0
1	1335.5
2	917.0
3	917.0
4	3018.5
5	5120.0
6	5120.0
7	5984.5
8	6849.0
9	6528.5
10	6208.0
11	6208.0
12	8271.5
13	10335.0
14	13386.0
15	16437.0
16	16437.0
17	24556.0
18	32675.0
19	32675.0
20	38331.5
21	43988.0
22	44770.5
23	45553.0
24	45553.0
25	53856.5
26	62160.0
27	62160.0
28	80560.5
29	98961.0
30	124747.0
31	150533.0
32	150533.0
33	179778.5
34	209024.0
35	209024.0
36	221655.0
37	234286.0
38	248932.0
39	263578.0
40	263578.0
41	256755.0
42	249932.0
43	255894.0
44	261856.0
45	261856.0
46	262537.0
47	263218.0
48	263218.0
49	267191.5
50	271165.0
51	261738.0
52	252311.0
53	252311.0
54	236254.5
55	220198.0
56	220198.0
57	209837.0
58	199476.0
59	175752.0
60	152028.0
61	152028.0
62	140892.0
63	129756.0
64	106088.5
65	82421.0
66	82421.0
67	69473.0
68	56525.0
69	56525.0
70	44496.0
71	32467.0
72	25650.0
73	18833.0
74	18833.0
75	13772.5
76	8712.0
77	8712.0
78	8820.5
79	8929.0
80	6762.5
81	4596.0
82	4596.0
83	4566.5
84	4537.0
85	4537.0
86	2860.0
87	1183.0
88	1029.0
89	875.0
90	875.0
91	523.0
92	171.0
93	127.5
94	84.0
95	84.0
96	70.5
97	57.0
98	57.0
99	51.0
100	45.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009537076190674619
2	0.0
3	0.0
4	1.1737939926984146E-4
5	2.9344849817460365E-5
6	5.868969963492073E-5
7	2.9344849817460365E-4
8	0.002171518886492067
9	0.0037854856264523864
10	0.0010270697436111126
11	0.0055755214653174685
12	3.5213819780952434E-4
13	2.0541394872222252E-4
14	2.9344849817460365E-5
15	0.0
16	1.1737939926984146E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	8.803454945238109E-5
22	2.9344849817460365E-5
23	5.868969963492073E-5
24	0.0
25	8.803454945238109E-5
26	2.9344849817460365E-5
27	2.0541394872222252E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	5.868969963492073E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	3407753.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.9614473518444
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.91948949853716	29.608810196073975
2	16.652702128687935	14.308483715351478
3	6.387492436361657	8.232477600451668
4	2.8922286745321024	4.970173197216225
5	1.57723027197568	3.38800476456092
6	0.9103141821877172	2.346504888701694
7	0.5985860734841446	1.800128685707544
8	0.39379324992400866	1.3534342379297601
9	0.26604129872030235	1.028656732354972
>10	1.189942602778854	8.887858756390715
>50	0.09151152364876954	2.7736960985368806
>100	0.09693748798131523	8.887958810672504
>500	0.014719832558805542	4.333907933779423
>1k	0.008666817300121485	6.319804666383383
>5k	2.0635279286003536E-4	0.6271843855146563
>10k+	1.375685285733569E-4	1.1329153303741468
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	22715	0.6665682636036121	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	15623	0.45845458869818323	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	8651	0.25386229577084957	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7029	0.20626494936692888	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	5544	0.16268784738800024	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG	4928	0.14461141990044468	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA	4773	0.1400629681787383	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	4175	0.12251474798789701	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	3915	0.11488508703535731	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.9344849817460362E-5	0.0
5	0.0	0.0	0.0	5.8689699634920724E-5	0.0
6	0.0	0.0	0.0	5.8689699634920724E-5	0.0
7	0.0	0.0	0.0	5.8689699634920724E-5	0.0
8	0.0	0.0	0.0	8.803454945238109E-5	0.0
9	0.0	0.0	0.0	1.1737939926984145E-4	0.0
10	0.0	0.0	0.0	1.1737939926984145E-4	0.0
11	0.0	0.0	0.0	1.467242490873018E-4	0.0
12	0.0	0.0	0.0	1.467242490873018E-4	0.0
13	0.0	0.0	0.0	1.467242490873018E-4	0.0
14	2.9344849817460362E-5	0.0	0.0	1.467242490873018E-4	0.0
15	2.9344849817460362E-5	0.0	0.0	1.467242490873018E-4	0.0
16	2.9344849817460362E-5	0.0	0.0	2.0541394872222252E-4	0.0
17	2.9344849817460362E-5	0.0	0.0	2.347587985396829E-4	0.0
18	2.9344849817460362E-5	0.0	0.0	2.934484981746036E-4	0.0
19	2.9344849817460362E-5	0.0	0.0	3.2279334799206397E-4	0.0
20	2.9344849817460362E-5	0.0	0.0	5.868969963492072E-4	0.0
21	2.9344849817460362E-5	0.0	0.0	0.001056414593428573	0.0
22	2.9344849817460362E-5	0.0	0.0	0.0016433115897777802	0.0
23	2.9344849817460362E-5	0.0	0.0	0.0036681062271825453	0.0
24	2.9344849817460362E-5	0.0	0.0	0.006397177260206359	0.0
25	2.9344849817460362E-5	0.0	0.0	0.00730686760454763	0.0
26	2.9344849817460362E-5	0.0	0.0	0.009654455589944459	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCCGTA	35	0.0020675727	22.856754	19
CTAGCGG	590	0.0	17.898003	29
TATATCG	45	0.008851614	17.77852	5
TAGCGGC	600	0.0	17.33304	30
TCTAGCG	615	0.0	16.910282	28
ACCGTCG	525	0.0	16.762604	8
TAAGACC	295	0.0	15.728547	4
TACCGTC	575	0.0	15.583259	7
AGGACCT	3150	0.0	15.543505	5
GACGCGT	95	1.4334699E-5	15.158527	8
TGTAGGA	4460	0.0	14.88764	2
AGCGGCG	690	0.0	14.840328	31
TAGACCG	65	0.0041626566	14.769846	5
GGACCGA	195	1.8189894E-12	14.769846	6
TCCTCGC	1420	0.0	14.647638	29
CGGTCCA	725	0.0	14.566124	10
ATACCGT	605	0.0	14.546061	6
GGACCTG	3310	0.0	14.502116	6
CCGTCGT	595	0.0	14.252696	9
ACCGTCC	135	1.3204044E-7	14.222816	8
>>END_MODULE