FastQCFastQC Report
Thu 2 Feb 2017
SRR4062778_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062778_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13091
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2962.261095409059659No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT550.42013597127797725No Hit
GTGTACATTCGTTCATTTCAACAATCATGCCAGAGTCA400.3055534336567107No Hit
CACATAAACCTGGTTTTAGCACGTCCATCAGCTCATTG380.29027576197387517No Hit
GTGCCATGTTGCTTTCCTGTTGGTGATCTTGGGCAAGT370.2826369261324574No Hit
GTATAAACGGTCCTCTGTTGACAGAGATTAGCATCACT360.27499809029103967No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGG360.27499809029103967No Hit
GTGATAAGGAGCGATAGAGCAAGACCTTGGACACCCTC340.2597204186082041No Hit
GTTCTGCTGTGTGATGCTGGAGCTGGACCGCGGATGAA330.2520815827667863No Hit
AATGTACACCCAGCACTTATCACCACCTCTGACTCAGT330.2520815827667863No Hit
GTTGCATAGTGCCATGTTGCTTTCCTGTTGGTGATCTT330.2520815827667863No Hit
CACTTGTGCTGGGCAAAGGGAATTCATCCGCGGTCCAG320.24444274692536858No Hit
ACCTTGGACACCCTCATCTGCTGTCTGCACATACACAT290.22152623940111527No Hit
GTAATAACACACTTTCTACTTTTGGCTTTCCTCTCTTT290.22152623940111527No Hit
TGTGTACAGGGGGCACACACGCCCATGTGTATGTGCAG290.22152623940111527No Hit
GTTTATACCTTCCCACCCATCTTGGGCAAACAAAAAAA280.2138874035596975No Hit
GGCCAGCAAGGGGCATGGGAAATGACGAAGGCAGACGT270.20624856771827974No Hit
GTCTGGAGACGACGATCAGAAATGGCGCCACGCAAGGG260.19860973187686196No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT250.1909708960354442No Hit
GTATCAACGCAGAGTACATGGGCTCTCTTTCCGGTGGA250.1909708960354442No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCG250.1909708960354442No Hit
GATTAGCATCACTTGTGCTGGGCAAAGGGAATTCATCC250.1909708960354442No Hit
GCCTTCACCTTCATCCCACCAGACACTCGGCAGATGGT250.1909708960354442No Hit
GGGCATGGGAAATGACGAAGGCAGACGTCCCTGCTGCC240.18333206019402642No Hit
GTCCTGTTCACAGACGGCGACCACGACGACCCCCTTTT240.18333206019402642No Hit
CTGTTCTGCTGTGTGATGCTGGAGCTGGACCGCGGATG240.18333206019402642No Hit
TATATACACTCGCTCCCAAGTACAATGTATAAACGGTC240.18333206019402642No Hit
GTCCAGCTCCAGCATCACACAGCAGAACAGTGAAGCAT230.17569322435260865No Hit
GGGCAAAGGGAATTCATCCGCGGTCCAGCTCCAGCATC230.17569322435260865No Hit
CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTT220.1680543885111909No Hit
GTGCTGGGTGTACATTCGTTCATTTCAACAATCATGCC220.1680543885111909No Hit
CTGCTAAGATGCCCCTGTTAAACTTTTTAGGAGGGGTC220.1680543885111909No Hit
GGTATAAACAGAACCAATCTAAAAATGGCTGCTATTAC220.1680543885111909No Hit
GTGTTATTACAGTAGAATTGATGTGGGACACACATAAA220.1680543885111909No Hit
ATATAATGATGGCTACAACGATTGGGAATCCTATTATT220.1680543885111909No Hit
GTACATGGGGTGGGGCCAGCAAGGGGCATGGGAAATGA220.1680543885111909No Hit
GTTTAAAGAGAGGAAAGCCAAAAGTAGAAAGTGTGTTA210.16041555266977314No Hit
CTTCTACATCTGGAAAGACTGAGGATTTAGTAACTTGC210.16041555266977314No Hit
GTATCAACGCAGAGTACATGGGAATTCCCATGACCAAA210.16041555266977314No Hit
CCCCTGGACCTGGAGCCCAGTCAGCCCTCAGAGCTCTT210.16041555266977314No Hit
CTATGCAACCATGCGACTCCCAAGCCCATGGGCAGCAG210.16041555266977314No Hit
GTCATCATCCCTTCTACATCTGGAAAGACTGAGGATTT200.15277671682835536No Hit
TGGTTGCATAGTGCCATGTTGCTTTCCTGTTGGTGATC200.15277671682835536No Hit
GCCCTACAACGACTACTTTGAATACTTTGGACCGGATT200.15277671682835536No Hit
TCCCTGAGGATGCCATCCCCGAAGAGAGTGGGGATGAA200.15277671682835536No Hit
ACATGGCACTATGCAACCATGCGACTCCCAAGCCCATG190.14513788098693758No Hit
TCTTAGCAGAGCTCCTAAGCAGGCACTTGGCAGTTCAG190.14513788098693758No Hit
GTATAGAAATGAGCTGTTTTATTACAAAGAGTTTCACA190.14513788098693758No Hit
GTTTTATTACAAAGAGTTTCACAAAATGAAAATATCTC190.14513788098693758No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTT180.13749904514551983No Hit
CCCATGACCAAAGTAAATACCATTGCCAGTTTAAAGAG180.13749904514551983No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTT180.13749904514551983No Hit
CCCAAGTACAATGTATAAACGGTCCTCTGTTGACAGAG180.13749904514551983No Hit
CCTCAGGGATGGCCTGCATCTGGACCCCAGGGGCATGG180.13749904514551983No Hit
CCTTATCACTGAGTCAGAGGTGGTGATAAGTGCTGGGT180.13749904514551983No Hit
ATGTATAAACGGTCCTCTGTTGACAGAGATTAGCATCA180.13749904514551983No Hit
GTCCAAGGTCTTGCTCTATCGCTCCTTATCACTGAGTC180.13749904514551983No Hit
GAGCTGGACCGCGGATGAATTCCCTTTGCCCAGCACAA180.13749904514551983No Hit
CCTCTGACTCAGTGATAAGGAGCGATAGAGCAAGACCT180.13749904514551983No Hit
CAGCAGAACAGTGAAGCATGCCAGTGCTGTGACAATGA180.13749904514551983No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT180.13749904514551983No Hit
TCACTGCCCTGCACATCAAACTCCGGGCCACAGGAGGA170.12986020930410205No Hit
CCTTTGTCCATGTTACCGATCTTTCTGGCAAGGAAACC170.12986020930410205No Hit
GAGCAAGACCTTGGACACCCTCATCTGCTGTCTGCACA170.12986020930410205No Hit
ATGTAGAAGGGATGATGACTCTGGCATGATTGTTGAAA170.12986020930410205No Hit
TCCTCAATCCGCCCAATCTTCATCCCAGAGCGAGCAAG170.12986020930410205No Hit
ATAATGAGAAGTCCTGTTCACAGACGGCGACCACGACG170.12986020930410205No Hit
CTATTACCCAAGCCTGAAAAGGGGTCCTAGGAGACCCA170.12986020930410205No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGA160.12222137346268429No Hit
GTATCAACGCAGAGTACATGGGGTGGGGCCAGCAAGGG160.12222137346268429No Hit
GTCCCAGGCTGATGACCTGCTCTTCCTTCTTTTCCTTC160.12222137346268429No Hit
GTACATGGGCTCTCTTTCCGGTGGAGGAGTCTGGAGAC160.12222137346268429No Hit
GTATCAACGCAGAGTACATGGGGGGGACCGGTTAGGTT160.12222137346268429No Hit
GGTGAAGGCTGACCGAGATGAGTCCTCTCCATATGCAG160.12222137346268429No Hit
CTTCACTGTTCTGCTGTGTGATGCTGGAGCTGGACCGC160.12222137346268429No Hit
CAGTAGAATTGATGTGGGACACACATAAACCTGGTTTT160.12222137346268429No Hit
TCTTTGCATCCTTCAATGACACCTTTGTCCATGTTACC160.12222137346268429No Hit
GTTCACAGACGGCGACCACGACGACCCCCTTTTCTTCG160.12222137346268429No Hit
GTTTTAACTCTTTTGGCTTTTTTGAAGTTAGAAGAGTT150.11458253762126652No Hit
ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACAT150.11458253762126652No Hit
TGCCAGTGCTGTGACAATGAGCTGATGGACGTGCTAAA150.11458253762126652No Hit
GAATTGATGTGGGACACACATAAACCTGGTTTTAGCAC150.11458253762126652No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGT150.11458253762126652No Hit
CTGCTGGACTTACGAAACAGCGGTGGCCCTGGACACAG150.11458253762126652No Hit
TGCTTACACAAAGCAATTTATTAACAGAAAATAATGAG150.11458253762126652No Hit
CATATATACACTCGCTCCCAAGTACAATGTATAAACGG150.11458253762126652No Hit
GTCTTGCTCTATCGCTCCTTATCACTGAGTCAGAGGTG150.11458253762126652No Hit
TCCCTGCTGCCCATGGGCTTGGGAGTCGCATGGTTGCA150.11458253762126652No Hit
GCAGATGAGGGTGTCCAAGGTCTTGCTCTATCGCTCCT140.10694370177984874No Hit
CCATTACATTTATTATTTTACTTCTACTCACAATTCTA140.10694370177984874No Hit
GATTGAGGATGTCACCCCCATTCCCTCTGATAGCACTC140.10694370177984874No Hit
CGCCAAGTGTGTGGAGTTCGTGAAGAGTTTCAACTTGC140.10694370177984874No Hit
TTGTAGGGCAGCTCATTAGGGATCTCTGTGTCCAGGGC140.10694370177984874No Hit
TAAATACACAGAGGAATAGAAAATATAAAATCTGAGAA140.10694370177984874No Hit
GTGTATATATGTGTGAGTGTGTGTGATACATGTGCACG140.10694370177984874No Hit
CTCCACACACTTGGCGTGTCCTTTGATGGTCAGATTGA140.10694370177984874No Hit
GCCCATGGGCTTGGGAGTCGCATGGTTGCATAGTGCCA140.10694370177984874No Hit
GCACTGGCATGCTTCACTGTTCTGCTGTGTGATGCTGG140.10694370177984874No Hit
GAAATGGCGCCACGCAAGGGGAAGGAAAAGAAGGAAGA140.10694370177984874No Hit
GGTCAAAGAAGAGGTCAAGTTGGCCTGAGCAAGGTCTG140.10694370177984874No Hit
GCTTAGGAGCTCTGCTAAGATGCCCCTGTTAAACTTTT140.10694370177984874No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTGT200.003556462732.1153831
GCGACCA200.003624481831.99233817
CGACCAC200.003624481831.99233818
Produced by FastQC (version 0.11.5)