FastQCFastQC Report
Thu 2 Feb 2017
SRR4062778_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062778_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13091
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAAT1230.9395768084943854No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTC570.43541364296081275No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT550.42013597127797725No Hit
CACATAAACCTGGTTTTAGCACGTCCATCAGCTCATTG470.35902528454663507No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGG420.3208311053395463No Hit
GTTGCATAGTGCCATGTTGCTTTCCTGTTGGTGATCTT410.3131922694981285No Hit
CACTTGTGCTGGGCAAAGGGAATTCATCCGCGGTCCAG400.3055534336567107No Hit
GTATAAACGGTCCTCTGTTGACAGAGATTAGCATCACT390.29791459781529295No Hit
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT370.2826369261324574No Hit
GTGTACATTCGTTCATTTCAACAATCATGCCAGAGTCA360.27499809029103967No Hit
GTGATAAGGAGCGATAGAGCAAGACCTTGGACACCCTC340.2597204186082041No Hit
TGTGTACAGGGGGCACACACGCCCATGTGTATGTGCAG330.2520815827667863No Hit
AATGTACACCCAGCACTTATCACCACCTCTGACTCAGT320.24444274692536858No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT310.2368039110839508No Hit
GTCCAGCTCCAGCATCACACAGCAGAACAGTGAAGCAT300.22916507524253305No Hit
TCTTAGCAGAGCTCCTAAGCAGGCACTTGGCAGTTCAG280.2138874035596975No Hit
CATATGGAGAGGACTCATCTCGGTCAGCCTTCACCTTC270.20624856771827974No Hit
GGTATAAACAGAACCAATCTAAAAATGGCTGCTATTAC270.20624856771827974No Hit
GTGTTATTACAGTAGAATTGATGTGGGACACACATAAA270.20624856771827974No Hit
GTTCTGCTGTGTGATGCTGGAGCTGGACCGCGGATGAA260.19860973187686196No Hit
CCTTATCACTGAGTCAGAGGTGGTGATAAGTGCTGGGT260.19860973187686196No Hit
GATTAGCATCACTTGTGCTGGGCAAAGGGAATTCATCC260.19860973187686196No Hit
TCCTCAATCCGCCCAATCTTCATCCCAGAGCGAGCAAG250.1909708960354442No Hit
CTATGCAACCATGCGACTCCCAAGCCCATGGGCAGCAG250.1909708960354442No Hit
GGCCAGCAAGGGGCATGGGAAATGACGAAGGCAGACGT250.1909708960354442No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCG240.18333206019402642No Hit
GTAATAACACACTTTCTACTTTTGGCTTTCCTCTCTTT240.18333206019402642No Hit
CATATATACACTCGCTCCCAAGTACAATGTATAAACGG230.17569322435260865No Hit
GTCCTGTTCACAGACGGCGACCACGACGACCCCCTTTT220.1680543885111909No Hit
GTGTGATGCTGGAGCTGGACCGCGGATGAATTCCCTTT220.1680543885111909No Hit
GTATAGAAATGAGCTGTTTTATTACAAAGAGTTTCACA220.1680543885111909No Hit
CAGCAGAACAGTGAAGCATGCCAGTGCTGTGACAATGA220.1680543885111909No Hit
GTATCAACGCAGAGTACATGGGGTGGGGCCAGCAAGGG210.16041555266977314No Hit
GTCTGGAGACGACGATCAGAAATGGCGCCACGCAAGGG210.16041555266977314No Hit
TATATACACTCGCTCCCAAGTACAATGTATAAACGGTC210.16041555266977314No Hit
GGGCATGGGAAATGACGAAGGCAGACGTCCCTGCTGCC200.15277671682835536No Hit
ACTAAATCCTCAGTCTTTCCAGATGTAGAAGGGATGAT200.15277671682835536No Hit
CCCATGACCAAAGTAAATACCATTGCCAGTTTAAAGAG200.15277671682835536No Hit
TGGTTGCATAGTGCCATGTTGCTTTCCTGTTGGTGATC200.15277671682835536No Hit
GTTTATACCTTCCCACCCATCTTGGGCAAACAAAAAAA200.15277671682835536No Hit
CTATCAGAGGGAATGGGGGTGACATCCTCAATCCGCCC200.15277671682835536No Hit
CCTCTGACTCAGTGATAAGGAGCGATAGAGCAAGACCT200.15277671682835536No Hit
GTTTTATTACAAAGAGTTTCACAAAATGAAAATATCTC200.15277671682835536No Hit
GCCTGGGACCTCAGGTGGCTGAGGGAGAGAATGTGTTT190.14513788098693758No Hit
GCCCTACAACGACTACTTTGAATACTTTGGACCGGATT190.14513788098693758No Hit
GTATCAACGCAGAGTACATGGGAATTCCCATGACCAAA190.14513788098693758No Hit
GTATATATGTGTGAGTGTGTGTGATACATGTGCACGTG190.14513788098693758No Hit
TGCTTACACAAAGCAATTTATTAACAGAAAATAATGAG190.14513788098693758No Hit
CGCCCATGTGTATGTGCAGACAGCAGATGAGGGTGTCC180.13749904514551983No Hit
GGTCATCAGCCTGGGACCTCAGGTGGCTGAGGGAGAGA180.13749904514551983No Hit
GCCCATGGGCTTGGGAGTCGCATGGTTGCATAGTGCCA180.13749904514551983No Hit
GCACTGGCATGCTTCACTGTTCTGCTGTGTGATGCTGG180.13749904514551983No Hit
GCCTTCACCTTCATCCCACCAGACACTCGGCAGATGGT180.13749904514551983No Hit
GTACATGGGAATTCCCATGACCAAAGTAAATACCATTG180.13749904514551983No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT180.13749904514551983No Hit
GTACATGGGGTGGGGCCAGCAAGGGGCATGGGAAATGA180.13749904514551983No Hit
ACCTTGGACACCCTCATCTGCTGTCTGCACATACACAT170.12986020930410205No Hit
GTAAATACCATTGCCAGTTTAAAGAGAGGAAAGCCAAA170.12986020930410205No Hit
CGTGTGTACAGGGGGCACACACGCCCATGTGTATGTGC170.12986020930410205No Hit
CCCCTGGACCTGGAGCCCAGTCAGCCCTCAGAGCTCTT170.12986020930410205No Hit
GTCCAAGGTCTTGCTCTATCGCTCCTTATCACTGAGTC170.12986020930410205No Hit
GTCCTCTGTTGACAGAGATTAGCATCACTTGTGCTGGG170.12986020930410205No Hit
TCCCTGAGGATGCCATCCCCGAAGAGAGTGGGGATGAA170.12986020930410205No Hit
GTTCACAGACGGCGACCACGACGACCCCCTTTTCTTCG170.12986020930410205No Hit
ACATGGCACTATGCAACCATGCGACTCCCAAGCCCATG160.12222137346268429No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGT160.12222137346268429No Hit
CTCTGATAGCACTCGAAGAAAAGGGGGTCGTCGTGGTC160.12222137346268429No Hit
GTGCCATGTTGCTTTCCTGTTGGTGATCTTGGGCAAGT160.12222137346268429No Hit
GTGTATATATGTGTGAGTGTGTGTGATACATGTGCACG160.12222137346268429No Hit
TATCAACGCAGAGTACATGGGAATTCCCATGACCAAAG160.12222137346268429No Hit
GTCCCAGGCTGATGACCTGCTCTTCCTTCTTTTCCTTC160.12222137346268429No Hit
TATCAACGCAGAGTACATGGGCTCTCTTTCCGGTGGAG160.12222137346268429No Hit
GTATCAACGCAGAGTACATGGGGGGGACCGGTTAGGTT160.12222137346268429No Hit
TCTTTGCATCCTTCAATGACACCTTTGTCCATGTTACC160.12222137346268429No Hit
ATGCAACCATGCGACTCCCAAGCCCATGGGCAGCAGGG150.11458253762126652No Hit
GTTTAAAGAGAGGAAAGCCAAAAGTAGAAAGTGTGTTA150.11458253762126652No Hit
TGCCAGTGCTGTGACAATGAGCTGATGGACGTGCTAAA150.11458253762126652No Hit
CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTT150.11458253762126652No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT150.11458253762126652No Hit
GATTGAGGATGTCACCCCCATTCCCTCTGATAGCACTC150.11458253762126652No Hit
TTTTAATTCTCAAGGGGTTTTTACTTTTATGGTTGTTA150.11458253762126652No Hit
GTATCAACGCAGAGTACATGGGCTCTCTTTCCGGTGGA150.11458253762126652No Hit
GTGCTGGGCAAAGGGAATTCATCCGCGGTCCAGCTCCA150.11458253762126652No Hit
TTACCGATCTTTCTGGCAAGGAAACCATCTGCCGAGTG150.11458253762126652No Hit
TTGTAGGGCAGCTCATTAGGGATCTCTGTGTCCAGGGC150.11458253762126652No Hit
GTATCAACGCAGAGTACATGGGGGTAGTTGAGAGAACG150.11458253762126652No Hit
ATCCTGGGCAGCCAACATGGCTGCATATGGAGAGGACT150.11458253762126652No Hit
ATAATGAGAAGTCCTGTTCACAGACGGCGACCACGACG150.11458253762126652No Hit
GTCCAGGGGTCTTGGTCCTGTTTCCTCCTGTGGCCCGG150.11458253762126652No Hit
CCCCCATTCCCTCTGATAGCACTCGAAGAAAAGGGGGT150.11458253762126652No Hit
GTGGAGGAGTCTGGAGACGACGATCAGAAATGGCGCCA150.11458253762126652No Hit
GGTGAAGGCTGACCGAGATGAGTCCTCTCCATATGCAG150.11458253762126652No Hit
GAGCTGGACCGCGGATGAATTCCCTTTGCCCAGCACAA150.11458253762126652No Hit
GCATAGTGCCATGTTGCTTTCCTGTTGGTGATCTTGGG150.11458253762126652No Hit
CCTTCATCCCACCAGACACTCGGCAGATGGTTTCCTTG150.11458253762126652No Hit
ACGCAGAGTACATGGGCTCTCTTTCCGGTGGAGGAGTC140.10694370177984874No Hit
CTCCATATGCAGCCATGTTGGCTGCCCAGGATGTGGCC140.10694370177984874No Hit
GTGTGATACATGTGCACGTGTGTACAGGGGGCACACAC140.10694370177984874No Hit
CCTTTGTCCATGTTACCGATCTTTCTGGCAAGGAAACC140.10694370177984874No Hit
GTTTTAACTCTTTTGGCTTTTTTGAAGTTAGAAGAGTT140.10694370177984874No Hit
GTGCTGGGTGTACATTCGTTCATTTCAACAATCATGCC140.10694370177984874No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTT140.10694370177984874No Hit
GGATTTAGTAACTTGCCCAAGATCACCAACAGGAAAGC140.10694370177984874No Hit
CCCAAGTACAATGTATAAACGGTCCTCTGTTGACAGAG140.10694370177984874No Hit
CTGCTAAGATGCCCCTGTTAAACTTTTTAGGAGGGGTC140.10694370177984874No Hit
TAAATACACAGAGGAATAGAAAATATAAAATCTGAGAA140.10694370177984874No Hit
GTACATGGGCTCTCTTTCCGGTGGAGGAGTCTGGAGAC140.10694370177984874No Hit
GTCTTGCTCTATCGCTCCTTATCACTGAGTCAGAGGTG140.10694370177984874No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA307.977319E-426.6666661
TCAACGC350.001959771822.8571434
ATCAACG350.001959771822.8571433
ATACACA350.001959771822.8571431
AACGCAG400.004254319720.06
TATCAAC400.004254319720.02
CAACGCA400.004254319720.05
ACGCAGA450.00840279217.7777797
CAGAGTA450.00840279217.77777910
AGAGTAC450.00840279217.77777911
GCAGAGT450.00840279217.7777799
CGCAGAG450.00840279217.7777798
Produced by FastQC (version 0.11.5)