FastQCFastQC Report
Thu 2 Feb 2017
SRR4062777_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062777_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4357710
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT82060.18830991507007122No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT69180.15875310656285066No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC66700.1530620440552492No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA57300.13149108132482426No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG55000.12621307980567775No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG53500.12277090490188654No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC53450.12265616573842683No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC52840.12125634794421841No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT49640.11391304148279716No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT49620.11386714581741327No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG49410.1133852413308825No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG47300.10854324863288287No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA44750.1026915512964378No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG12150.019.22551728
CTAGCGG12350.019.04459629
TAGCGGC12900.017.98455430
AGCGGCG14500.016.11039731
CAAGACG16750.015.66212754
GTATCAA71300.015.6115341
AAGACGG16950.015.5718775
GTATTAG10650.015.4895171
GCGCAAG16550.015.3868391
CGCAAGA16700.015.2393552
TATACTG5700.015.1546395
CATCGTT11100.015.1344928
GTATAGG3650.014.9188831
CGCTTCG11300.014.86730532
ATCGTTT11200.014.8571929
CGGTCCA16950.014.72554310
CTATTCC15600.014.6633484
TCGTTTA11250.014.64893630
GTCCTAT16950.014.5512831
GCGGCGC15650.014.51761832
Produced by FastQC (version 0.11.5)