##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062777_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4357710
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.277511353440225	32.0	32.0	32.0	32.0	32.0
2	30.91591386301521	32.0	32.0	32.0	32.0	32.0
3	30.96210142483093	32.0	32.0	32.0	32.0	32.0
4	31.027132599461645	32.0	32.0	32.0	32.0	32.0
5	30.94393660890697	32.0	32.0	32.0	32.0	32.0
6	34.60003258592242	36.0	36.0	36.0	32.0	36.0
7	34.541435754100206	36.0	36.0	36.0	32.0	36.0
8	34.529054250971264	36.0	36.0	36.0	32.0	36.0
9	34.65737095859981	36.0	36.0	36.0	32.0	36.0
10	34.392493534448136	36.0	36.0	36.0	32.0	36.0
11	34.62738479614293	36.0	36.0	36.0	32.0	36.0
12	34.4623304442012	36.0	36.0	36.0	32.0	36.0
13	34.54399099527045	36.0	36.0	36.0	32.0	36.0
14	34.44400132179516	36.0	36.0	36.0	32.0	36.0
15	34.39598068710401	36.0	36.0	36.0	32.0	36.0
16	34.40698968035964	36.0	36.0	36.0	32.0	36.0
17	34.33191263301138	36.0	36.0	36.0	32.0	36.0
18	34.35576942935624	36.0	36.0	36.0	32.0	36.0
19	34.350786766443846	36.0	36.0	36.0	32.0	36.0
20	34.335820878397136	36.0	36.0	36.0	32.0	36.0
21	34.319906785903605	36.0	36.0	36.0	32.0	36.0
22	34.29661955476615	36.0	36.0	36.0	32.0	36.0
23	34.23932134079597	36.0	36.0	36.0	32.0	36.0
24	34.2184209137368	36.0	36.0	36.0	32.0	36.0
25	34.19638411000273	36.0	36.0	36.0	32.0	36.0
26	34.14718372723288	36.0	36.0	36.0	32.0	36.0
27	34.150866395423286	36.0	36.0	36.0	32.0	36.0
28	34.13547230081855	36.0	36.0	36.0	32.0	36.0
29	34.103420144984405	36.0	36.0	36.0	32.0	36.0
30	34.08004777738766	36.0	36.0	36.0	32.0	36.0
31	34.101371591959996	36.0	36.0	36.0	32.0	36.0
32	34.05088383577613	36.0	36.0	36.0	32.0	36.0
33	34.021424096601194	36.0	36.0	36.0	32.0	36.0
34	34.0349135210925	36.0	36.0	36.0	32.0	36.0
35	33.98606033903128	36.0	36.0	36.0	32.0	36.0
36	33.95192245468377	36.0	36.0	36.0	32.0	36.0
37	33.95533640375335	36.0	36.0	36.0	32.0	36.0
38	33.36306706963061	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	4.0
8	11.0
9	18.0
10	43.0
11	43.0
12	27.0
13	20.0
14	324.0
15	857.0
16	1254.0
17	1465.0
18	1865.0
19	2366.0
20	3307.0
21	5050.0
22	7615.0
23	11723.0
24	17903.0
25	26812.0
26	39948.0
27	57230.0
28	81019.0
29	109448.0
30	148443.0
31	200051.0
32	274726.0
33	412408.0
34	929753.0
35	2023976.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.89179107220974	16.842009249081894	11.214190135538015	26.052009543170346
2	17.06677981699404	19.029621139273175	37.194014237470576	26.70958480626221
3	19.482712600847655	22.871271881356588	27.782601221750436	29.86341429604532
4	12.715928633684706	14.852622124145224	35.376381445272074	37.055067796898
5	15.085548431778456	35.96074724620081	33.27401314498657	15.679691177034162
6	35.38085934362593	34.77078953153707	16.36985858129682	13.478492543540192
7	31.30965575956179	29.757143086621184	20.262890371318882	18.67031078249815
8	28.632004473922805	32.540376258376845	18.997397019572496	19.830222248127853
9	27.3114424326038	14.403994216216304	18.240466704806042	40.04409664637386
10	16.00731121305095	26.063900828191084	30.952385323416763	26.976402635341202
11	38.32978170203841	20.934537231545093	21.627529877534123	19.108151188882378
12	25.308181446624378	23.254380471358065	28.01381225676	23.423625825257556
13	29.360180028620054	18.874017835318796	25.1940460192996	26.571756116761552
14	23.79259111019515	19.56807715593075	24.496328694840344	32.14300303903375
15	25.485916902471406	26.80372262385028	21.894791301329995	25.815569172348322
16	26.50873306723167	25.492193208899593	23.094386527960868	24.90468719590786
17	24.54912673753329	25.63989861419663	24.641533521100122	25.169441127169957
18	25.605670295322142	24.345000338501116	25.709584400950558	24.33974496522618
19	25.75476026058796	24.82199727037619	24.977893810886687	24.445348658149157
20	25.932953478317145	24.033058769207326	24.58058057782392	25.45340717465161
21	27.178699723483607	24.010316179708806	24.124856251177587	24.68612784563
22	25.973631958169513	23.95827691584607	24.78627143367277	25.281819692311647
23	24.475988931685492	23.76559536942921	25.336452388094195	26.421963310791103
24	24.93265018812963	24.82592787591385	24.842269012752055	25.39915292320446
25	25.263280385003096	24.201184681401124	24.866558089233024	25.668976844362756
26	24.868415737413542	25.273749433083676	25.315154981610792	24.54267984789199
27	25.710238030432315	24.387068997567784	24.540373466209445	25.36231950579046
28	24.73087458530773	24.42792203142757	25.49301793159666	25.348185451668044
29	24.643488933790014	24.73499206376164	25.4252244775807	25.196294524867646
30	24.658863692118242	24.90914085353554	25.654120451104735	24.777875003241487
31	25.365798468555262	24.726135950029533	24.30240227435562	25.60566330705958
32	25.016994024437427	24.58090968634149	24.301661490048915	26.100434799172167
33	24.575113779199846	24.23098204570997	25.067615746568496	26.126288428521693
34	25.551381865693685	24.483743991825925	25.105153804436757	24.859720338043633
35	26.15674192143166	24.158679942401026	25.251100407571965	24.433477728595356
36	24.62088376033184	25.132423587475994	24.714915466712387	25.531777185479775
37	26.000992816880363	24.671155227125794	24.642192330615824	24.685659625378015
38	24.793141868361236	24.472082901601965	25.150529380108345	25.584245849928454
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	182.0
1	274.0
2	366.0
3	366.0
4	947.0
5	1528.0
6	1528.0
7	1828.5
8	2129.0
9	2091.0
10	2053.0
11	2053.0
12	2849.0
13	3645.0
14	5310.0
15	6975.0
16	6975.0
17	10544.0
18	14113.0
19	14113.0
20	17956.0
21	21799.0
22	24336.5
23	26874.0
24	26874.0
25	33269.5
26	39665.0
27	39665.0
28	49730.0
29	59795.0
30	73092.5
31	86390.0
32	86390.0
33	112132.5
34	137875.0
35	137875.0
36	155680.0
37	173485.0
38	200168.0
39	226851.0
40	226851.0
41	243703.5
42	260556.0
43	297925.5
44	335295.0
45	335295.0
46	346952.5
47	358610.0
48	358610.0
49	384000.0
50	409390.0
51	416513.0
52	423636.0
53	423636.0
54	406019.5
55	388403.0
56	388403.0
57	376654.5
58	364906.0
59	328427.5
60	291949.0
61	291949.0
62	275648.0
63	259347.0
64	215816.0
65	172285.0
66	172285.0
67	142975.0
68	113665.0
69	113665.0
70	91342.5
71	69020.0
72	53838.5
73	38657.0
74	38657.0
75	28775.5
76	18894.0
77	18894.0
78	18640.0
79	18386.0
80	14225.0
81	10064.0
82	10064.0
83	10232.0
84	10400.0
85	10400.0
86	6880.5
87	3361.0
88	2963.0
89	2565.0
90	2565.0
91	1835.5
92	1106.0
93	958.5
94	811.0
95	811.0
96	731.5
97	652.0
98	652.0
99	1339.5
100	2027.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12111866094806677
2	0.05603860743372092
3	0.011909925167117592
4	0.003120905246104032
5	6.884349807582423E-5
6	2.753739923032969E-4
7	0.0
8	2.983218249952383E-4
9	2.0653049422747268E-4
10	4.589566538388282E-4
11	0.0020194092768908442
12	0.0030061660826443248
13	0.01434239543246338
14	0.00927092440754433
15	0.022901937026557528
16	0.010969064026747993
17	0.020354727597752028
18	0.006081175663364473
19	0.00764162828641649
20	0.005920540834520884
21	0.006494236651819419
22	0.007205619465269602
23	0.009339767905620154
24	0.013952282276700378
25	0.01739445718049159
26	0.018495953149704775
27	0.008444802430634439
28	0.0060123321652886495
29	0.01106085535751576
30	0.0032356444095637386
31	0.005874645169137001
32	0.006792558476814657
33	0.008421854597942497
34	0.011955820832501475
35	0.01578810889205569
36	0.015352100070908804
37	0.009431559236387919
38	0.005851697336445059
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	4357710.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.01448835667548
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.45823030513947	33.51699141129362
2	14.052368842012338	12.651203872449477
3	4.881139396331678	6.591659775704469
4	2.230471338011656	4.016141042992963
5	1.1466333158280184	2.580755602235827
6	0.7036741943358165	1.9005320296693564
7	0.45815327952922125	1.443647482685857
8	0.32556197995449265	1.172400476483018
9	0.25600465147856244	1.037152656291286
>10	1.3041555578957085	10.564021505137658
>50	0.08762036240263597	2.7349017971590706
>100	0.07116731027625198	6.695291200904256
>500	0.013022559833016659	4.126116598815196
>1k	0.011388356080286526	9.84268463742581
>5k	4.0855089077261055E-4	1.12649991075226
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	8206	0.18830991507007122	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	6918	0.15875310656285066	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	6670	0.1530620440552492	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	5730	0.13149108132482426	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	5500	0.12621307980567775	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	5350	0.12277090490188654	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	5345	0.12265616573842683	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	5284	0.12125634794421841	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	4964	0.11391304148279716	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	4962	0.11386714581741327	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	4941	0.1133852413308825	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	4730	0.10854324863288287	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	4475	0.1026915512964378	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.179133076776564E-5
2	0.0	0.0	0.0	2.294783269194141E-5	9.179133076776564E-5
3	0.0	0.0	0.0	2.294783269194141E-5	9.179133076776564E-5
4	0.0	0.0	0.0	2.294783269194141E-5	9.179133076776564E-5
5	0.0	0.0	0.0	2.294783269194141E-5	1.1473916345970705E-4
6	0.0	0.0	0.0	2.294783269194141E-5	2.0653049422747268E-4
7	0.0	0.0	0.0	4.589566538388282E-5	2.0653049422747268E-4
8	0.0	0.0	0.0	4.589566538388282E-5	2.294783269194141E-4
9	0.0	0.0	0.0	4.589566538388282E-5	2.294783269194141E-4
10	0.0	0.0	0.0	6.884349807582423E-5	2.9832182499523835E-4
11	0.0	0.0	0.0	6.884349807582423E-5	2.9832182499523835E-4
12	0.0	0.0	0.0	6.884349807582423E-5	7.343306461421251E-4
13	2.294783269194141E-5	0.0	0.0	6.884349807582423E-5	7.80226311526008E-4
14	2.294783269194141E-5	0.0	0.0	9.179133076776564E-5	7.80226311526008E-4
15	2.294783269194141E-5	0.0	0.0	1.1473916345970705E-4	0.0011014959692131876
16	2.294783269194141E-5	0.0	0.0	2.294783269194141E-4	0.001124443801905129
17	2.294783269194141E-5	0.0	0.0	2.753739923032969E-4	0.0011703394672890118
18	2.294783269194141E-5	0.0	0.0	3.2126965768717973E-4	0.0012391829653648362
19	2.294783269194141E-5	0.0	0.0	3.2126965768717973E-4	0.0012391829653648362
20	2.294783269194141E-5	0.0	0.0	4.1306098845494537E-4	0.0013309742961326017
21	2.294783269194141E-5	0.0	0.0	5.966436499904767E-4	0.0014227656269003674
22	2.294783269194141E-5	0.0	0.0	7.343306461421251E-4	0.0014457134595923088
23	2.294783269194141E-5	0.0	0.0	0.0011932872999809534	0.001514556957668133
24	2.294783269194141E-5	0.0	0.0	0.00162929612112784	0.001514556957668133
25	6.884349807582423E-5	0.0	0.0	0.0018587744480472541	0.001514556957668133
26	6.884349807582423E-5	0.0	0.0	0.0024554180980377307	0.0015834004557439573
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	1215	0.0	19.225517	28
CTAGCGG	1235	0.0	19.044596	29
TAGCGGC	1290	0.0	17.984554	30
AGCGGCG	1450	0.0	16.110397	31
CAAGACG	1675	0.0	15.6621275	4
GTATCAA	7130	0.0	15.611534	1
AAGACGG	1695	0.0	15.571877	5
GTATTAG	1065	0.0	15.489517	1
GCGCAAG	1655	0.0	15.386839	1
CGCAAGA	1670	0.0	15.239355	2
TATACTG	570	0.0	15.154639	5
CATCGTT	1110	0.0	15.13449	28
GTATAGG	365	0.0	14.918883	1
CGCTTCG	1130	0.0	14.867305	32
ATCGTTT	1120	0.0	14.85719	29
CGGTCCA	1695	0.0	14.725543	10
CTATTCC	1560	0.0	14.663348	4
TCGTTTA	1125	0.0	14.648936	30
GTCCTAT	1695	0.0	14.551283	1
GCGGCGC	1565	0.0	14.517618	32
>>END_MODULE