FastQCFastQC Report
Thu 2 Feb 2017
SRR4062777_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062777_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4357710
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT82580.18950320237005217No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC73480.1686206746203855No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT70700.16224117713202577No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC58720.13474967356707995No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA58470.13417597774978143No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC57950.13298269044980046No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG54660.12543285349415173No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT53280.12226605258266383No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG52370.12017779980769716No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT49920.1145555807981715No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG49150.11278859768089203No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC48920.11226079752897737No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA48000.11014959692131876No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG45600.10464211707525282No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG44710.10259975996567004No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG44180.10138352483299715No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA43710.10030497669647591No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG13800.019.01363428
CTAGCGG14450.018.2690729
TAGCGGC15000.017.49254430
ATCGTTT10350.016.69489929
CATCGTT10650.016.52507428
TAAACGC11000.016.43562128
CAAGACG16700.016.2885594
AAGACGG16900.016.191225
AGCGGCG16600.016.0956631
CGACCCG9050.015.5598075
CGCTTCG11300.015.43293132
ATACCGT13200.015.396076
TACCGTC13100.015.3914437
ACCGTCG12600.015.3672068
CGCCGGT17250.015.3064677
CGTTTAT11400.015.1572131
CGGTCCA17950.015.15408310
CGCAAGA17100.015.064342
CGTCGTA12850.014.94250210
GTAAACG12050.014.87069727
Produced by FastQC (version 0.11.5)