##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062777_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4357710
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.20227275334981	32.0	32.0	32.0	32.0	32.0
2	31.257850568303077	32.0	32.0	32.0	32.0	32.0
3	31.35655768740921	32.0	32.0	32.0	32.0	32.0
4	31.461946985916914	32.0	32.0	32.0	32.0	32.0
5	31.373757776446805	32.0	32.0	32.0	32.0	32.0
6	34.9067097168008	36.0	36.0	36.0	36.0	36.0
7	34.93193466293076	36.0	36.0	36.0	36.0	36.0
8	34.861179151435046	36.0	36.0	36.0	32.0	36.0
9	34.98526106601862	36.0	36.0	36.0	36.0	36.0
10	34.82320599580972	36.0	36.0	36.0	32.0	36.0
11	35.00822840436835	36.0	36.0	36.0	36.0	36.0
12	34.895221802276886	36.0	36.0	36.0	32.0	36.0
13	34.9581670189159	36.0	36.0	36.0	36.0	36.0
14	34.90099960759206	36.0	36.0	36.0	32.0	36.0
15	34.86549242606782	36.0	36.0	36.0	32.0	36.0
16	34.88081400552125	36.0	36.0	36.0	32.0	36.0
17	34.83959602635329	36.0	36.0	36.0	32.0	36.0
18	34.84486438978271	36.0	36.0	36.0	32.0	36.0
19	34.84414222148789	36.0	36.0	36.0	32.0	36.0
20	34.82697839002596	36.0	36.0	36.0	32.0	36.0
21	34.82117121148493	36.0	36.0	36.0	32.0	36.0
22	34.80534477971228	36.0	36.0	36.0	32.0	36.0
23	34.74796005241285	36.0	36.0	36.0	32.0	36.0
24	34.72075860945313	36.0	36.0	36.0	32.0	36.0
25	34.700059205408344	36.0	36.0	36.0	32.0	36.0
26	34.632084741756564	36.0	36.0	36.0	32.0	36.0
27	34.620828370864515	36.0	36.0	36.0	32.0	36.0
28	34.590897512684414	36.0	36.0	36.0	32.0	36.0
29	34.552048208806916	36.0	36.0	36.0	32.0	36.0
30	34.53196128241668	36.0	36.0	36.0	32.0	36.0
31	34.52990423869418	36.0	36.0	36.0	32.0	36.0
32	34.50425361026778	36.0	36.0	36.0	32.0	36.0
33	34.47491319982284	36.0	36.0	36.0	32.0	36.0
34	34.467336743381274	36.0	36.0	36.0	32.0	36.0
35	34.434309304657724	36.0	36.0	36.0	32.0	36.0
36	34.40446059971866	36.0	36.0	36.0	32.0	36.0
37	34.40752941338455	36.0	36.0	36.0	32.0	36.0
38	33.96110961950198	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	16.0
21	87.0
22	329.0
23	1041.0
24	2852.0
25	7082.0
26	14877.0
27	29207.0
28	50582.0
29	80035.0
30	121899.0
31	176952.0
32	255361.0
33	413108.0
34	1002788.0
35	2201491.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.18298289150482	18.04751510622726	11.904962443563218	26.8645395587047
2	16.05584125607257	20.015604526230522	36.47087575813902	27.45767845955789
3	18.37554587157016	23.588191963210033	28.133446236670178	29.902815928549632
4	12.20463523804388	15.731996544052432	35.347206843969474	36.71616137393421
5	14.562583228939793	36.5081743640982	32.833789498105546	16.095452908856466
6	35.39230080441481	35.18783523976628	16.023628967744926	13.39623498807399
7	31.12479014081792	29.987511714938485	20.158598007565487	18.729100136678106
8	28.49381392410379	32.86839181762292	18.76210095949407	19.875693298779215
9	27.211543779473384	14.25095346380624	18.009731653208362	40.52777110351201
10	15.77772790400737	26.26996824907267	30.983246084832526	26.969057762087427
11	38.48542297235552	20.884280851413433	21.501262074851514	19.129034101379528
12	25.165609265628152	23.32532528076183	27.896753721059543	23.612311732550474
13	29.42340198884824	18.849929481115947	25.118904522525426	26.607764007510387
14	23.704479510788698	19.485793908173747	24.462584459536984	32.34714212150057
15	25.448894029203412	26.95099031371982	21.723244548168648	25.876871108908116
16	26.496376880604007	25.523388551185956	22.95630571017936	25.023928858030676
17	24.576096160598112	25.73741712963919	24.468080712117143	25.218405997645554
18	25.613957789756547	24.398870048718248	25.585181207560854	24.401990953964354
19	25.82790043394352	24.863701347726213	24.841832063170795	24.466566155159477
20	25.987594401646735	23.96166794027138	24.483914716674583	25.566822941407295
21	27.226568676203954	24.033602106430845	24.0066613075742	24.733167909791007
22	25.918745799109487	24.058547304809615	24.6951435697719	25.327563326309
23	24.434673237103695	23.675924529929887	25.342514321198266	26.546887911768152
24	24.97346083149177	24.81399634211547	24.76530104114317	25.44724178524959
25	25.21436737586244	24.211293740330348	24.855554734532344	25.71878414927487
26	24.925276102649352	25.185160367523395	25.243287241070938	24.646276288756315
27	25.754915891487812	24.351860521045026	24.447989124548155	25.445234462919004
28	24.742835112937758	24.36132739443423	25.504496627815985	25.391340864812022
29	24.717340070817013	24.6094393614995	25.4366857822113	25.236534785472188
30	24.700634048617278	24.79547744113307	25.627978915531322	24.875909594718326
31	25.39579274435426	24.59048445169596	24.330669089957798	25.68305371399198
32	25.13065348543157	24.506013479556923	24.309212866390833	26.054120168620678
33	24.562075034823337	24.150597446824136	25.096897223541724	26.190430294810806
34	25.50988936849859	24.373512693593653	25.169595957509795	24.94700198039796
35	26.22212125175838	24.140661035268522	25.143527219571748	24.493690493401353
36	24.77700443581606	25.023349419764052	24.718051453630462	25.48159469078943
37	26.053523524970686	24.651319156162295	24.537796227835262	24.757361091031758
38	24.901054614273058	24.259111500612594	25.129248019658046	25.710585865456302
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	123.0
1	154.5
2	186.0
3	186.0
4	692.5
5	1199.0
6	1199.0
7	1527.0
8	1855.0
9	1870.0
10	1885.0
11	1885.0
12	2591.0
13	3297.0
14	5023.5
15	6750.0
16	6750.0
17	10466.5
18	14183.0
19	14183.0
20	17832.5
21	21482.0
22	24227.5
23	26973.0
24	26973.0
25	33077.5
26	39182.0
27	39182.0
28	49666.5
29	60151.0
30	73127.0
31	86103.0
32	86103.0
33	112526.0
34	138949.0
35	138949.0
36	156111.0
37	173273.0
38	199491.5
39	225710.0
40	225710.0
41	243005.5
42	260301.0
43	297897.5
44	335494.0
45	335494.0
46	347858.5
47	360223.0
48	360223.0
49	384997.5
50	409772.0
51	416710.5
52	423649.0
53	423649.0
54	406452.5
55	389256.0
56	389256.0
57	377801.5
58	366347.0
59	329799.0
60	293251.0
61	293251.0
62	277831.5
63	262412.0
64	217206.0
65	172000.0
66	172000.0
67	143861.0
68	115722.0
69	115722.0
70	92700.0
71	69678.0
72	54044.5
73	38411.0
74	38411.0
75	28428.5
76	18446.0
77	18446.0
78	18341.0
79	18236.0
80	14058.5
81	9881.0
82	9881.0
83	9448.5
84	9016.0
85	9016.0
86	5627.0
87	2238.0
88	1900.0
89	1562.0
90	1562.0
91	943.5
92	325.0
93	213.5
94	102.0
95	102.0
96	68.5
97	35.0
98	35.0
99	43.5
100	52.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009202080909468505
2	0.0
3	0.0
4	1.1473916345970705E-4
5	2.294783269194141E-5
6	1.8358266153553127E-4
7	5.966436499904766E-4
8	0.0023177311018860824
9	0.00406176638647363
10	9.638089730615392E-4
11	0.004658410036464106
12	2.983218249952383E-4
13	2.753739923032969E-4
14	4.589566538388282E-5
15	0.0
16	2.294783269194141E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	1.6063482884358987E-4
22	6.884349807582423E-5
23	2.0653049422747268E-4
24	0.0
25	9.179133076776564E-5
26	2.294783269194141E-5
27	1.3768699615164846E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	6.884349807582423E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	4357710.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.03303291059578
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.64221583713321	31.26014864817955
2	14.96178557313269	12.877020219397922
3	5.276116392225234	6.81141871040287
4	2.3195660249501935	3.99271844359926
5	1.2900416907592016	2.7757203267240675
6	0.7584467039144625	1.9582957182290373
7	0.5145719688096133	1.5500514728057908
8	0.35962451436887294	1.2380586849834188
9	0.2748013952992828	1.0642983739012508
>10	1.4032252450561788	10.822658850601403
>50	0.09719838630071968	2.894668385736782
>100	0.07576518272871074	6.903589984038885
>500	0.013962496578884268	4.2989909701529365
>1k	0.012197123347411503	10.258096558210266
>5k	4.8146539529255925E-4	1.2942646530365765
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	8258	0.18950320237005217	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	7348	0.1686206746203855	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	7070	0.16224117713202577	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	5872	0.13474967356707995	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	5847	0.13417597774978143	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	5795	0.13298269044980046	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	5466	0.12543285349415173	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	5328	0.12226605258266383	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	5237	0.12017779980769716	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	4992	0.1145555807981715	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	4915	0.11278859768089203	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	4892	0.11226079752897737	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	4800	0.11014959692131876	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	4560	0.10464211707525282	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	4471	0.10259975996567004	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	4418	0.10138352483299715	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	4371	0.10030497669647591	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8358266153553127E-4
2	0.0	0.0	0.0	0.0	1.8358266153553127E-4
3	0.0	0.0	0.0	0.0	1.8358266153553127E-4
4	0.0	0.0	0.0	0.0	1.8358266153553127E-4
5	0.0	0.0	0.0	0.0	2.0653049422747268E-4
6	0.0	0.0	0.0	0.0	2.9832182499523835E-4
7	0.0	0.0	0.0	2.294783269194141E-5	2.9832182499523835E-4
8	0.0	0.0	0.0	2.294783269194141E-5	2.9832182499523835E-4
9	0.0	0.0	0.0	2.294783269194141E-5	2.9832182499523835E-4
10	0.0	0.0	0.0	4.589566538388282E-5	3.2126965768717973E-4
11	0.0	0.0	0.0	4.589566538388282E-5	3.2126965768717973E-4
12	0.0	0.0	0.0	4.589566538388282E-5	7.80226311526008E-4
13	0.0	0.0	0.0	4.589566538388282E-5	8.94965474985715E-4
14	0.0	0.0	0.0	6.884349807582423E-5	8.94965474985715E-4
15	0.0	0.0	0.0	9.179133076776564E-5	0.0011932872999809534
16	0.0	0.0	0.0	2.294783269194141E-4	0.0012162351326728948
17	0.0	0.0	0.0	2.753739923032969E-4	0.0012162351326728948
18	0.0	0.0	0.0	2.9832182499523835E-4	0.001285078630748719
19	0.0	0.0	0.0	3.2126965768717973E-4	0.001285078630748719
20	0.0	0.0	0.0	3.6716532307106255E-4	0.0014227656269003674
21	0.0	0.0	0.0	5.507479846065938E-4	0.0014457134595923088
22	0.0	0.0	0.0	7.113828134501837E-4	0.001514556957668133
23	0.0	0.0	0.0	0.0011014959692131876	0.0015834004557439573
24	0.0	0.0	0.0	0.001514556957668133	0.0015834004557439573
25	0.0	0.0	0.0	0.0017669831172794886	0.0015834004557439573
26	2.294783269194141E-5	0.0	0.0	0.002294783269194141	0.0017669831172794886
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	1380	0.0	19.013634	28
CTAGCGG	1445	0.0	18.26907	29
TAGCGGC	1500	0.0	17.492544	30
ATCGTTT	1035	0.0	16.694899	29
CATCGTT	1065	0.0	16.525074	28
TAAACGC	1100	0.0	16.435621	28
CAAGACG	1670	0.0	16.288559	4
AAGACGG	1690	0.0	16.19122	5
AGCGGCG	1660	0.0	16.09566	31
CGACCCG	905	0.0	15.559807	5
CGCTTCG	1130	0.0	15.432931	32
ATACCGT	1320	0.0	15.39607	6
TACCGTC	1310	0.0	15.391443	7
ACCGTCG	1260	0.0	15.367206	8
CGCCGGT	1725	0.0	15.306467	7
CGTTTAT	1140	0.0	15.15721	31
CGGTCCA	1795	0.0	15.154083	10
CGCAAGA	1710	0.0	15.06434	2
CGTCGTA	1285	0.0	14.942502	10
GTAAACG	1205	0.0	14.870697	27
>>END_MODULE