FastQCFastQC Report
Thu 2 Feb 2017
SRR4062776_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062776_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2128101
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT51310.24110697753537075No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT34180.1606126776877601No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT21540.10121700050890442No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATTA450.00885447717.77715332
ATCGTTT4000.015.59945129
CCTATTC5950.015.5964553
GTATCAA41950.014.9321161
TCTAGCG4350.014.71212528
CATCGTT4350.014.71212528
ACCGTCG4400.014.5442598
CTATTCC6300.014.473984
TACCGTC4650.014.4504257
ATACCGT4800.014.3311446
TAGCGGC4250.014.30537930
GTCCTAT6950.014.0611741
CTAGCGG4450.014.02197729
TTCGGAA5550.013.54907231
TGTACCG2500.013.4379475
CCGTCGT4650.013.4188829
TAACGAA2750.013.38134713
CCGATAA2650.013.2825519
CGAACGA2900.013.24060216
CGGCTAA1105.707203E-513.0904489
Produced by FastQC (version 0.11.5)