##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062776_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2128101
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27436291792542	32.0	32.0	32.0	32.0	32.0
2	30.895826372902413	32.0	32.0	32.0	32.0	32.0
3	30.94701708236592	32.0	32.0	32.0	32.0	32.0
4	31.01391757252123	32.0	32.0	32.0	32.0	32.0
5	30.92555381534993	32.0	32.0	32.0	32.0	32.0
6	34.60076941836877	36.0	36.0	36.0	32.0	36.0
7	34.54048656525231	36.0	36.0	36.0	32.0	36.0
8	34.52396761243945	36.0	36.0	36.0	32.0	36.0
9	34.65794950521615	36.0	36.0	36.0	32.0	36.0
10	34.386211932610344	36.0	36.0	36.0	32.0	36.0
11	34.65005561296198	36.0	36.0	36.0	32.0	36.0
12	34.4746743693086	36.0	36.0	36.0	32.0	36.0
13	34.55074265742086	36.0	36.0	36.0	32.0	36.0
14	34.45663528187807	36.0	36.0	36.0	32.0	36.0
15	34.40686085857767	36.0	36.0	36.0	32.0	36.0
16	34.421240345265566	36.0	36.0	36.0	32.0	36.0
17	34.355324770769805	36.0	36.0	36.0	32.0	36.0
18	34.37839933349028	36.0	36.0	36.0	32.0	36.0
19	34.373610087115225	36.0	36.0	36.0	32.0	36.0
20	34.34867142114025	36.0	36.0	36.0	32.0	36.0
21	34.34386243885981	36.0	36.0	36.0	32.0	36.0
22	34.31860564888603	36.0	36.0	36.0	32.0	36.0
23	34.26414535776262	36.0	36.0	36.0	32.0	36.0
24	34.24711797043467	36.0	36.0	36.0	32.0	36.0
25	34.22656302496921	36.0	36.0	36.0	32.0	36.0
26	34.188736342870946	36.0	36.0	36.0	32.0	36.0
27	34.18970481194267	36.0	36.0	36.0	32.0	36.0
28	34.175600688125236	36.0	36.0	36.0	32.0	36.0
29	34.14126068264617	36.0	36.0	36.0	32.0	36.0
30	34.11899999107185	36.0	36.0	36.0	32.0	36.0
31	34.12647191087265	36.0	36.0	36.0	32.0	36.0
32	34.08872229278592	36.0	36.0	36.0	32.0	36.0
33	34.0575033797738	36.0	36.0	36.0	32.0	36.0
34	34.06409564207714	36.0	36.0	36.0	32.0	36.0
35	34.02829705920912	36.0	36.0	36.0	32.0	36.0
36	33.98421409510169	36.0	36.0	36.0	32.0	36.0
37	33.98838354006694	36.0	36.0	36.0	32.0	36.0
38	33.40957689508158	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	4.0
8	4.0
9	7.0
10	12.0
11	23.0
12	14.0
13	9.0
14	242.0
15	578.0
16	750.0
17	850.0
18	1024.0
19	1362.0
20	1759.0
21	2463.0
22	3719.0
23	5810.0
24	8771.0
25	13327.0
26	19434.0
27	27850.0
28	38988.0
29	52404.0
30	70827.0
31	95166.0
32	130127.0
33	196502.0
34	448159.0
35	1007914.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.07899615134891	17.250990392487132	11.605847314883647	26.06416614128031
2	16.491831874613574	19.598611101315786	38.08812501028509	25.82143201378555
3	18.49209101837831	23.927759402284202	28.926692589741492	28.653456989595995
4	12.529193447885994	15.529457539921598	36.28187548577427	35.65947352641813
5	14.459907391482913	36.57162405114802	33.98349136177	14.984977195599075
6	33.95156985399155	35.85167210501782	16.901117242831152	13.29564079815948
7	30.045265751264395	30.602570651083433	21.204793573983167	18.147370023669012
8	27.864195784676703	33.42358628123865	19.49773811576844	19.21447981831621
9	27.617611055897413	14.138889476268682	18.52375011453906	39.719749353294844
10	15.57198446870928	26.901293224772814	32.21069963643472	25.31602267008319
11	37.152311781268914	21.19934813745503	22.52285654136921	19.125483539906845
12	24.429254750003643	23.943599096074294	29.107056174875506	22.520089979046553
13	29.532631923260027	19.473270392220353	25.221953662194302	25.77214402232531
14	23.42305212443395	19.716923851971043	25.499833639424264	31.360190384170743
15	25.04499169257596	27.117312107688658	22.60700361672577	25.230692583009613
16	25.46277389414322	25.84273042354462	23.973118721729424	24.72137696058274
17	24.04072610645319	25.913237770582658	25.36150928923892	24.684526833725233
18	24.705143182602107	25.115813949554084	26.226725602002272	23.952317265841536
19	25.463029972649604	25.171809355526943	25.671635478443942	23.693525193379514
20	25.571339956202593	24.53632083008299	25.182332540719365	24.710006672995046
21	26.591524806447442	24.489291462540677	24.78421973942363	24.134963991588247
22	25.6835693353409	24.401536127762522	25.093940265457615	24.820954271438964
23	24.335069636992852	24.409932458978183	25.60256817545251	25.652429728576447
24	24.728101234412406	25.10614179622843	25.384690579157766	24.7810663902014
25	24.732745858301023	24.561579132886852	25.591070379508874	25.114604629303255
26	24.500003289915846	25.373287951294206	25.814982652743744	24.31172610604621
27	25.133640080886614	25.017188170403266	24.924515382883826	24.924656365826298
28	24.423738347533792	24.923601363356756	25.767640061034324	24.88502022807513
29	24.445512806956447	25.236257717235144	25.5972295260935	24.7209999497149
30	24.58748309472499	25.156130024238333	25.74996452124355	24.506422359793124
31	24.872931375423875	25.048308633899502	24.960526018992624	25.118233971683996
32	24.67108246611199	25.070243166593436	24.825502299144958	25.433172068149617
33	24.374399941351097	24.76934015753434	25.416686835059828	25.439573066054738
34	24.97289503576163	24.842997989979505	25.57167154093895	24.612435433319906
35	25.483946315550526	24.74566059159673	25.468343103356712	24.30204998949603
36	24.559661320597765	25.37524779184451	25.102712010655164	24.96237887690256
37	25.29746505587441	25.183314793902152	25.040262757078935	24.478957393144498
38	24.556985343356626	25.08156808085802	25.46493688606597	24.89650968971939
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	392.0
1	360.0
2	328.0
3	328.0
4	766.0
5	1204.0
6	1204.0
7	1418.5
8	1633.0
9	1603.5
10	1574.0
11	1574.0
12	2086.5
13	2599.0
14	3600.0
15	4601.0
16	4601.0
17	7079.0
18	9557.0
19	9557.0
20	11909.5
21	14262.0
22	16045.5
23	17829.0
24	17829.0
25	20701.0
26	23573.0
27	23573.0
28	29689.5
29	35806.0
30	43164.5
31	50523.0
32	50523.0
33	62491.0
34	74459.0
35	74459.0
36	82925.5
37	91392.0
38	104149.5
39	116907.0
40	116907.0
41	127112.5
42	137318.0
43	152949.0
44	168580.0
45	168580.0
46	174757.5
47	180935.0
48	180935.0
49	190706.0
50	200477.0
51	200542.0
52	200607.0
53	200607.0
54	191369.5
55	182132.0
56	182132.0
57	176131.5
58	170131.0
59	151702.5
60	133274.0
61	133274.0
62	123157.5
63	113041.0
64	93158.5
65	73276.0
66	73276.0
67	60907.0
68	48538.0
69	48538.0
70	38718.5
71	28899.0
72	22587.5
73	16276.0
74	16276.0
75	12393.5
76	8511.0
77	8511.0
78	7820.0
79	7129.0
80	5627.0
81	4125.0
82	4125.0
83	4050.5
84	3976.0
85	3976.0
86	2625.5
87	1275.0
88	1123.5
89	972.0
90	972.0
91	712.5
92	453.0
93	407.5
94	362.0
95	362.0
96	318.0
97	274.0
98	274.0
99	587.5
100	901.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12598086275040518
2	0.05807995015274181
3	0.012546396999014615
4	0.002584463801295145
5	1.4097075279791702E-4
6	2.3495125466319503E-4
7	9.3980501865278E-5
8	3.75922007461112E-4
9	2.8194150559583404E-4
10	4.6990250932639005E-4
11	0.002114561291968755
12	0.00234951254663195
13	0.013721153272330588
14	0.009069118429999327
15	0.022837261953262557
16	0.010619796710776416
17	0.019923866395438937
18	0.005591839860984041
19	0.0075654304001548794
20	0.0056858203628493195
21	0.005920771617512515
22	0.007189508392693768
23	0.009820962444921552
24	0.013486202017667395
25	0.01649357807735629
26	0.018185227110931297
27	0.008646206171605577
28	0.005544849610051403
29	0.010995718718237528
30	0.0031483468124868137
31	0.005497859359118764
32	0.006390674126838905
33	0.008646206171605577
34	0.012311445744351421
35	0.015694743811501426
36	0.014426007036320174
37	0.009586011190258358
38	0.005826791115647236
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2128101.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.43597123996891
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.184042953389	44.68828370781924
2	12.616039222023536	14.2399685339288
3	3.7390088512433786	6.330437879842814
4	1.5310364603337823	3.456221185709645
5	0.7917613837079156	2.2341911339928955
6	0.47553157906687693	1.610225191194916
7	0.3062837250898996	1.209979365031088
8	0.22519777143339548	1.0167403961536159
9	0.16090263930217025	0.8172627051683133
>10	0.8282812557130118	8.314104572448064
>50	0.06837535108488163	2.698445836171972
>100	0.0625945085002171	7.472265437365469
>500	0.0077696169268338345	3.027297067961647
>1k	0.003091137917127429	2.6426553703207114
>5k	8.354426803047105E-5	0.24192161689085573
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5131	0.24110697753537075	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3418	0.1606126776877601	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2154	0.10121700050890442	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.6990250932639E-5
2	0.0	0.0	0.0	0.0	4.6990250932639E-5
3	0.0	0.0	0.0	0.0	4.6990250932639E-5
4	0.0	0.0	0.0	0.0	1.4097075279791702E-4
5	0.0	0.0	0.0	0.0	1.4097075279791702E-4
6	0.0	0.0	0.0	0.0	2.3495125466319503E-4
7	0.0	0.0	0.0	4.6990250932639E-5	2.3495125466319503E-4
8	0.0	0.0	0.0	4.6990250932639E-5	2.3495125466319503E-4
9	0.0	0.0	0.0	9.3980501865278E-5	2.3495125466319503E-4
10	0.0	0.0	0.0	9.3980501865278E-5	2.3495125466319503E-4
11	0.0	0.0	0.0	9.3980501865278E-5	2.3495125466319503E-4
12	0.0	0.0	0.0	1.4097075279791702E-4	2.8194150559583404E-4
13	0.0	0.0	0.0	1.4097075279791702E-4	2.8194150559583404E-4
14	0.0	0.0	0.0	1.4097075279791702E-4	2.8194150559583404E-4
15	0.0	4.6990250932639E-5	0.0	1.4097075279791702E-4	3.2893175652847303E-4
16	0.0	4.6990250932639E-5	0.0	2.3495125466319503E-4	4.6990250932639005E-4
17	0.0	4.6990250932639E-5	0.0	2.8194150559583404E-4	4.6990250932639005E-4
18	0.0	4.6990250932639E-5	0.0	4.6990250932639005E-4	4.6990250932639005E-4
19	0.0	4.6990250932639E-5	0.0	5.16892760259029E-4	4.6990250932639005E-4
20	0.0	4.6990250932639E-5	0.0	7.048537639895851E-4	6.10873262124307E-4
21	0.0	4.6990250932639E-5	0.0	7.988342658548631E-4	6.10873262124307E-4
22	0.0	4.6990250932639E-5	0.0	9.86795269585419E-4	6.10873262124307E-4
23	0.0	4.6990250932639E-5	0.0	0.0014566977789118093	7.048537639895851E-4
24	0.0	4.6990250932639E-5	0.0	0.002114561291968755	7.048537639895851E-4
25	0.0	4.6990250932639E-5	0.0	0.0023495125466319504	7.048537639895851E-4
26	0.0	4.6990250932639E-5	0.0	0.0027724248050257014	7.988342658548631E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATTA	45	0.008854477	17.777153	32
ATCGTTT	400	0.0	15.599451	29
CCTATTC	595	0.0	15.596455	3
GTATCAA	4195	0.0	14.932116	1
TCTAGCG	435	0.0	14.712125	28
CATCGTT	435	0.0	14.712125	28
ACCGTCG	440	0.0	14.544259	8
CTATTCC	630	0.0	14.47398	4
TACCGTC	465	0.0	14.450425	7
ATACCGT	480	0.0	14.331144	6
TAGCGGC	425	0.0	14.305379	30
GTCCTAT	695	0.0	14.061174	1
CTAGCGG	445	0.0	14.021977	29
TTCGGAA	555	0.0	13.549072	31
TGTACCG	250	0.0	13.437947	5
CCGTCGT	465	0.0	13.418882	9
TAACGAA	275	0.0	13.381347	13
CCGATAA	265	0.0	13.282551	9
CGAACGA	290	0.0	13.240602	16
CGGCTAA	110	5.707203E-5	13.090448	9
>>END_MODULE