FastQCFastQC Report
Thu 2 Feb 2017
SRR4062776_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062776_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2128101
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT57140.2685022938290993No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT42390.19919167370345672No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT24390.11460922202470654No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG450.008847102517.7797245
CCGTCGT4050.016.9895119
CGTTTAT4000.016.79946931
ACCGTCG4350.016.5535348
AAATCCG1102.0222615E-715.99949429
CGTCGTA4350.015.81670710
GTCCTAG2950.015.7286871
ATCGTTT4300.015.62741529
ATACCGT4750.015.4964326
TACCGTC4650.015.4855647
GTATCAA37550.015.1263761
TTCGTTC5550.014.99051829
GTCGTAG5000.014.72022711
ATAACGA1851.0913936E-1114.70223912
CGACGGT5500.014.5470477
CGTAAAC1103.5468074E-614.54499432
GTATTAA3050.014.1638191
CATCGTT4750.014.14692228
GTATTAT2150.014.1394221
CGAACGA2053.6379788E-1214.04833616
Produced by FastQC (version 0.11.5)