FastQCFastQC Report
Thu 2 Feb 2017
SRR4062775_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062775_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2813321
Sequences flagged as poor quality0
Sequence length38
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA255610.9085703337798993No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA216120.7682024198447316No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC214270.7616265616330309No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG185610.6597540771209542No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA113100.40201598040180986No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA105000.3732243849884176No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA99380.3532479940966566No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA83430.2965534327579398No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA78550.2792073851508591No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT65360.23232329336040927No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA63500.2257118899691859No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAAT61820.2197402998093712No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG60990.21679004990898657No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCA55220.19628048132438497No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT54750.19460985788681773No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACG48990.17413583448173883No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT48950.173993653763648No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAG48870.17370929232746637No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAAT47110.16745334073147003No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGT46390.16489408780583517No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTT45550.16190829272592783No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAA44670.15878031692792965No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAA44440.1579627777989074No Hit
GACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAA42830.15224000389575168No Hit
ACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAA42590.1513869195872067No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTC41740.14836557932777666No Hit
CCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA40540.14410015778505192No Hit
ACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT40290.14321152829698422No Hit
CACTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAA37640.13379205572346703No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT34920.1241237668932909No Hit
CCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGT33080.1175834538611129No Hit
AGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCT33040.11744127314302208No Hit
ATACACACTTTAGGACGTGAAATATGGCGAGGAAAACT32910.11697918580922688No Hit
GAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTT31790.11299812570268378No Hit
CTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGT30810.10951469810945853No Hit
GAAATACACACTTTAGGACGTGAAATATGGCGAGGAAA30090.10695544518382366No Hit
ATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCAC29750.10574690908005166No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAA28720.1020857555892129No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC61400.022.9527454
TTAGACG350.00206835522.8550993
GGCATCG2000.022.3995926
GACGTTA1200.021.3310457
CGGCATC2050.021.07278425
CTACGTC707.808594E-720.567034
CATCGTT2200.020.36217728
GTCCTAG7950.020.3487381
TTGCGAA400.00448692519.99856818
GCATCGT2250.019.90968327
GTGTAGG5150.019.9047161
CCGGTGT659.440373E-619.69264813
TTCGCGT659.443273E-619.69194626
AGGACCT113600.019.2068185
CGTTTTG3600.019.1104230
GGACGTC1600.018.9959376
GGACCTA4300.018.9726876
ACGCTTC1351.8189894E-1218.96227831
GACGTAG1105.2204996E-1018.9070637
GGACCTG112250.018.5831076
Produced by FastQC (version 0.11.5)