##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062775_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2813321
Sequences flagged as poor quality	0
Sequence length	38
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.41629838898583	32.0	32.0	32.0	32.0	32.0
2	31.190554863806867	32.0	32.0	32.0	32.0	32.0
3	31.219126790010808	32.0	32.0	32.0	32.0	32.0
4	31.258354450132067	32.0	32.0	32.0	32.0	32.0
5	31.26561739666394	32.0	32.0	32.0	32.0	32.0
6	34.97601766737603	36.0	36.0	36.0	36.0	36.0
7	34.90590906618903	36.0	36.0	36.0	36.0	36.0
8	34.90694272000955	36.0	36.0	36.0	36.0	36.0
9	34.97355829640485	36.0	36.0	36.0	36.0	36.0
10	34.789836282457635	36.0	36.0	36.0	32.0	36.0
11	34.941384221708084	36.0	36.0	36.0	36.0	36.0
12	34.82195668393333	36.0	36.0	36.0	36.0	36.0
13	34.853613576268046	36.0	36.0	36.0	36.0	36.0
14	34.823193300728924	36.0	36.0	36.0	32.0	36.0
15	34.793194946470734	36.0	36.0	36.0	32.0	36.0
16	34.82231142482497	36.0	36.0	36.0	36.0	36.0
17	34.80825259542014	36.0	36.0	36.0	32.0	36.0
18	34.84471946144787	36.0	36.0	36.0	36.0	36.0
19	34.73392371506842	36.0	36.0	36.0	32.0	36.0
20	34.703476069741065	36.0	36.0	36.0	32.0	36.0
21	34.647778906139756	36.0	36.0	36.0	32.0	36.0
22	34.6006196946598	36.0	36.0	36.0	32.0	36.0
23	34.62517643738486	36.0	36.0	36.0	32.0	36.0
24	34.57684281317347	36.0	36.0	36.0	32.0	36.0
25	34.54025082811382	36.0	36.0	36.0	32.0	36.0
26	34.566444070904105	36.0	36.0	36.0	32.0	36.0
27	34.48527914162657	36.0	36.0	36.0	32.0	36.0
28	34.36712341037514	36.0	36.0	36.0	32.0	36.0
29	34.29263706487813	36.0	36.0	36.0	32.0	36.0
30	34.21203161672628	36.0	36.0	36.0	32.0	36.0
31	34.21502203267953	36.0	36.0	36.0	32.0	36.0
32	34.18087982139258	36.0	36.0	36.0	32.0	36.0
33	34.2553878494491	36.0	36.0	36.0	32.0	36.0
34	34.212475931470316	36.0	36.0	36.0	32.0	36.0
35	34.2236406723584	36.0	36.0	36.0	32.0	36.0
36	34.10971837198812	36.0	36.0	36.0	32.0	36.0
37	33.99646609825185	36.0	36.0	36.0	32.0	36.0
38	33.42942913375331	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	4.0
9	10.0
10	20.0
11	26.0
12	17.0
13	16.0
14	178.0
15	447.0
16	661.0
17	742.0
18	914.0
19	1111.0
20	1465.0
21	2027.0
22	3052.0
23	4509.0
24	7152.0
25	11390.0
26	17599.0
27	26900.0
28	39751.0
29	56764.0
30	79834.0
31	109284.0
32	153559.0
33	239818.0
34	613730.0
35	1442337.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.97798256172763	20.225242571935187	12.53269943004226	26.264075436294927
2	10.362798419714588	19.545113108202887	51.97564129740465	18.116447174677873
3	19.97570519458774	25.434357632760236	29.972409718348764	24.61752745430326
4	10.130288099751636	16.909122513042853	40.950374783122726	32.01021460408279
5	9.449030769750276	41.300138804222065	37.774760380547505	11.476070045480153
6	29.547700361708905	39.80632080622412	19.080962225305974	11.565016606760999
7	26.29511527479445	31.39300492194101	22.882635859896542	19.429243943368
8	20.339571053924306	48.05059086898938	19.564435395409117	12.045402681677196
9	27.003161750461647	14.502393084315123	21.47697644949108	37.01746871573216
10	15.743968128600303	29.046431493765695	36.52876722261993	18.680833155014064
11	34.00648997267586	18.483696689573637	31.618017938600122	15.891795399150382
12	28.134835183037932	21.800115311800404	35.807193266700835	14.257856238460823
13	32.52268051535847	23.679705866982452	25.795013432866064	18.002600184793007
14	16.96922600572971	28.01372314887327	26.529643729224116	28.487407116172903
15	17.933744959265248	44.99385974331096	21.01240637619614	16.059988921227653
16	15.932924256283062	26.951804817563037	39.88451471187868	17.230756214275228
17	16.648795205139017	33.550552178386575	33.21869762553694	16.58195499093746
18	17.181307013247014	25.88507167379625	42.69743441721451	14.236186895742229
19	25.02864264606505	24.173326844673497	27.208149832587598	23.58988067667385
20	24.412741836754282	29.6045582935619	30.991042806696246	14.991657062987576
21	19.47842401767712	27.17043092244647	26.153058090495705	27.198086969380704
22	22.477251091499703	36.04835516095755	26.901050868400787	14.573342879141956
23	16.361456901755325	34.56828524890172	32.06554621729248	17.004711632050473
24	24.59050253848432	26.589792239252102	33.68101734189532	15.13868788036826
25	19.40315328126689	31.304500543227853	33.459138457687956	15.833207717817304
26	13.78179774234296	34.278651520812986	35.2870280534383	16.65252268340575
27	16.24220995952076	33.087609272081124	32.562520284943695	18.107660483454417
28	15.05855847369392	34.61517270013092	27.900045891273585	22.426222934901578
29	19.346309128998737	29.185479745508413	26.961184593852572	24.507026531640278
30	15.428605146659974	30.434815063901365	32.16779786452783	21.968781924910832
31	23.819470315063857	26.54791222916424	29.818061215494122	19.81455624027778
32	17.758479418868983	33.029342708401835	30.893362742989584	18.318815129739598
33	15.413565239894819	33.35101260643557	35.81090808780532	15.424514065864297
34	17.97265215898208	32.50257914656022	34.65765073343036	14.867117961027335
35	16.060485724514358	34.17356689653015	33.6994534683765	16.066493910578995
36	14.482213590493437	31.799103978203107	36.1008547117934	17.617827719510053
37	15.95187243336708	31.636416895127805	28.513656477868654	23.898054193636455
38	18.138382316661772	36.71943817820267	27.6637017646511	17.478477740484454
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	76.0
1	141.0
2	206.0
3	206.0
4	616.5
5	1027.0
6	1027.0
7	1321.5
8	1616.0
9	1702.0
10	1788.0
11	1788.0
12	2447.5
13	3107.0
14	4326.0
15	5545.0
16	5545.0
17	8264.5
18	10984.0
19	10984.0
20	15050.0
21	19116.0
22	26374.0
23	33632.0
24	33632.0
25	57863.0
26	82094.0
27	82094.0
28	117976.0
29	153858.0
30	219955.5
31	286053.0
32	286053.0
33	356518.0
34	426983.0
35	426983.0
36	440572.0
37	454161.0
38	442659.0
39	431157.0
40	431157.0
41	357747.0
42	284337.0
43	221186.0
44	158035.0
45	158035.0
46	129446.0
47	100857.0
48	100857.0
49	89859.0
50	78861.0
51	71535.0
52	64209.0
53	64209.0
54	56328.5
55	48448.0
56	48448.0
57	45702.5
58	42957.0
59	37222.5
60	31488.0
61	31488.0
62	31655.5
63	31823.0
64	26141.0
65	20459.0
66	20459.0
67	17462.0
68	14465.0
69	14465.0
70	11641.5
71	8818.0
72	7036.0
73	5254.0
74	5254.0
75	3926.0
76	2598.0
77	2598.0
78	2757.0
79	2916.0
80	2339.5
81	1763.0
82	1763.0
83	1858.5
84	1954.0
85	1954.0
86	1288.0
87	622.0
88	550.0
89	478.0
90	478.0
91	354.0
92	230.0
93	203.0
94	176.0
95	176.0
96	160.5
97	145.0
98	145.0
99	585.0
100	1025.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12934890828312873
2	0.0596092660595787
3	0.01329389714149221
4	0.0033412468751344055
5	2.1327107713623862E-4
6	3.1990661570435795E-4
7	0.0
8	2.488162566589451E-4
9	2.1327107713623862E-4
10	5.331776928405965E-4
11	0.0020616204123169732
12	0.002808069182293809
13	0.01578205970808166
14	0.009383927393994499
15	0.026125706949189233
16	0.011445547806311473
17	0.022855550433100242
18	0.005651683544110324
19	0.007393397340722939
20	0.005793864262201149
21	0.006860219647882342
22	0.00778439431547271
23	0.010059285804925922
24	0.015000065758582117
25	0.01795031565896675
26	0.020687294482215145
27	0.009739379189221565
28	0.005331776928405966
29	0.01208536103772019
30	0.002808069182293809
31	0.00629149677551904
32	0.006611403391223398
33	0.008317572008313307
34	0.013329442321014913
35	0.017488228325171568
36	0.015746514528558953
37	0.010556918318243812
38	0.006646948570746105
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2813321.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.84752563809712
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.30211038302552	31.201453510047195
2	12.25616087992316	9.767553697747621
3	3.686560464059527	4.4070093782402155
4	1.597263104237088	2.5458792998749575
5	0.9102879325382744	1.8136360864934655
6	0.5485969743637082	1.3116139200564223
7	0.3893644459175875	1.0860646818885185
8	0.2925934715718409	0.9327300687999016
9	0.22613696300837044	0.8109898588077714
>10	1.4508316585648093	11.265188953870206
>50	0.16954755389780557	4.704803707862791
>100	0.14356013731481268	11.621888338043705
>500	0.014744023875024838	3.9493683284185566
>1k	0.010901641895472842	8.483400978960416
>5k	8.042194840922588E-4	2.2183061673741835
>10k+	5.361463227281726E-4	3.8801130235141597
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	25561	0.9085703337798993	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA	21612	0.7682024198447316	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	21427	0.7616265616330309	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG	18561	0.6597540771209542	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	11310	0.40201598040180986	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA	10500	0.3732243849884176	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	9938	0.3532479940966566	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA	8343	0.2965534327579398	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA	7855	0.2792073851508591	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT	6536	0.23232329336040927	No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	6350	0.2257118899691859	No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAAT	6182	0.2197402998093712	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG	6099	0.21679004990898657	No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCA	5522	0.19628048132438497	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5475	0.19460985788681773	No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACG	4899	0.17413583448173883	No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT	4895	0.173993653763648	No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAG	4887	0.17370929232746637	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAAT	4711	0.16745334073147003	No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGT	4639	0.16489408780583517	No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTT	4555	0.16190829272592783	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAA	4467	0.15878031692792965	No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAA	4444	0.1579627777989074	No Hit
GACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAA	4283	0.15224000389575168	No Hit
ACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAA	4259	0.1513869195872067	No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTC	4174	0.14836557932777666	No Hit
CCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	4054	0.14410015778505192	No Hit
ACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT	4029	0.14321152829698422	No Hit
CACTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAA	3764	0.13379205572346703	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3492	0.1241237668932909	No Hit
CCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGT	3308	0.1175834538611129	No Hit
AGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCT	3304	0.11744127314302208	No Hit
ATACACACTTTAGGACGTGAAATATGGCGAGGAAAACT	3291	0.11697918580922688	No Hit
GAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTT	3179	0.11299812570268378	No Hit
CTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGT	3081	0.10951469810945853	No Hit
GAAATACACACTTTAGGACGTGAAATATGGCGAGGAAA	3009	0.10695544518382366	No Hit
ATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCAC	2975	0.10574690908005166	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAA	2872	0.1020857555892129	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	7.109035904541288E-5	0.0
13	0.0	0.0	0.0	7.109035904541288E-5	0.0
14	0.0	0.0	0.0	1.0663553856811931E-4	0.0
15	0.0	0.0	0.0	1.0663553856811931E-4	0.0
16	0.0	0.0	0.0	1.777258976135322E-4	0.0
17	0.0	0.0	0.0	2.1327107713623862E-4	0.0
18	0.0	0.0	0.0	2.1327107713623862E-4	0.0
19	0.0	0.0	0.0	2.1327107713623862E-4	0.0
20	0.0	0.0	0.0	3.1990661570435795E-4	0.0
21	0.0	0.0	0.0	3.909969747497708E-4	0.0
22	0.0	0.0	0.0	7.464487699768353E-4	0.0
23	0.0	0.0	0.0	9.952650266357803E-4	0.0
24	0.0	0.0	0.0	0.0015995330785217898	0.0
25	0.0	0.0	0.0	0.0022393463099305054	0.0
26	0.0	0.0	0.0	0.0031635209775208732	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	6140	0.0	22.952745	4
TTAGACG	35	0.002068355	22.855099	3
GGCATCG	200	0.0	22.39959	26
GACGTTA	120	0.0	21.331045	7
CGGCATC	205	0.0	21.072784	25
CTACGTC	70	7.808594E-7	20.56703	4
CATCGTT	220	0.0	20.362177	28
GTCCTAG	795	0.0	20.348738	1
TTGCGAA	40	0.004486925	19.998568	18
GCATCGT	225	0.0	19.909683	27
GTGTAGG	515	0.0	19.904716	1
CCGGTGT	65	9.440373E-6	19.692648	13
TTCGCGT	65	9.443273E-6	19.691946	26
AGGACCT	11360	0.0	19.206818	5
CGTTTTG	360	0.0	19.11042	30
GGACGTC	160	0.0	18.995937	6
GGACCTA	430	0.0	18.972687	6
ACGCTTC	135	1.8189894E-12	18.962278	31
GACGTAG	110	5.2204996E-10	18.907063	7
GGACCTG	11225	0.0	18.583107	6
>>END_MODULE