FastQCFastQC Report
Thu 2 Feb 2017
SRR4062775_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062775_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2813321
Sequences flagged as poor quality0
Sequence length38
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA270590.9618170127049136No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC206250.7331193276558203No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA198650.7061049912185634No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG196510.6984983228007042No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA121950.43347346427940503No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA106930.38008460463629995No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA97180.3454280546016612No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA92880.3301436274068974No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA68850.24472856101383383No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT68240.24256030506294873No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA68110.24209821772915355No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG64130.2279512362791164No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT60000.2132710771362386No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCA59060.20992983026110423No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAAT56350.2002970866104508No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAG54800.19478758378443128No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT52570.18686100875086775No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACG51250.1821690450538705No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAA49600.17630409043262393No Hit
ACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAA48330.17178985263324023No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGT46950.16688461785910674No Hit
GACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAA46240.16436091011299456No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTT46170.16411209385633563No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAA45240.16080639216072393No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAAT44520.15824713923508907No Hit
CCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA43700.15533243451422712No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTC43200.1535551755380918No Hit
ACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT42260.1502139286629574No Hit
CACTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAA40750.14484660655502873No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT40120.14260726024509823No Hit
ATACACACTTTAGGACGTGAAATATGGCGAGGAAAACT36410.12941999864217413No Hit
GAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTT35870.127500558947948No Hit
CCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGT34510.12266641453285991No Hit
AGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCT33930.12060479412054294No Hit
ATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCAC33880.12042706822292942No Hit
GAAATACACACTTTAGGACGTGAAATATGGCGAGGAAA33110.11769008939968102No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAA32970.11719245688636314No Hit
CTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGT31450.11178958959891176No Hit
ACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAA29630.10532036692577917No Hit
TCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAG28610.10169475861446312No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCAA350.002067245522.85720811
TAGGACC63300.021.966874
TCGGACG604.7215544E-621.334914
TCTAGCG2800.021.14216628
AAGACGG2650.019.9267085
CTAGCGG3000.019.73268529
TTCATCG507.2010694E-419.1993728
TAGCGGC3100.019.09614830
AGCGGCG3100.019.09614831
AGGACCT114550.018.9841825
GCGCAAG2800.018.857531
GACGTGC852.3963185E-718.8255887
GGACCTG113800.018.5749666
GTCCTAG8100.018.5682831
GCGGCGC3400.018.3523432
CAAGACG2900.018.208244
GTGTAGG6250.018.1763741
CTAGGAC8650.018.1281853
TGTAGGA205750.017.9872472
ATACCCG450.00884779217.7797226
Produced by FastQC (version 0.11.5)