##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062775_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2813321
Sequences flagged as poor quality	0
Sequence length	38
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.274360089019346	32.0	32.0	32.0	32.0	32.0
2	31.453758387329422	32.0	32.0	32.0	32.0	32.0
3	31.502254097559433	32.0	32.0	32.0	32.0	32.0
4	31.577195776806132	32.0	32.0	32.0	32.0	32.0
5	31.551240331266854	32.0	32.0	32.0	32.0	32.0
6	35.118325637209544	36.0	36.0	36.0	36.0	36.0
7	35.13890380799063	36.0	36.0	36.0	36.0	36.0
8	35.062041622694316	36.0	36.0	36.0	36.0	36.0
9	35.1612180053396	36.0	36.0	36.0	36.0	36.0
10	35.0658261179581	36.0	36.0	36.0	36.0	36.0
11	35.17286296160303	36.0	36.0	36.0	36.0	36.0
12	35.12280219711864	36.0	36.0	36.0	36.0	36.0
13	35.14635194490781	36.0	36.0	36.0	36.0	36.0
14	35.119969957214266	36.0	36.0	36.0	36.0	36.0
15	35.08563189198815	36.0	36.0	36.0	36.0	36.0
16	35.11297004501086	36.0	36.0	36.0	36.0	36.0
17	35.13105365509304	36.0	36.0	36.0	36.0	36.0
18	35.13784029621931	36.0	36.0	36.0	36.0	36.0
19	35.072871527991296	36.0	36.0	36.0	36.0	36.0
20	35.05239039555031	36.0	36.0	36.0	36.0	36.0
21	35.01109400598083	36.0	36.0	36.0	36.0	36.0
22	34.981756081158174	36.0	36.0	36.0	36.0	36.0
23	34.97362298863158	36.0	36.0	36.0	36.0	36.0
24	34.95674045016548	36.0	36.0	36.0	36.0	36.0
25	34.919872634512735	36.0	36.0	36.0	36.0	36.0
26	34.87807576881558	36.0	36.0	36.0	36.0	36.0
27	34.79480514310312	36.0	36.0	36.0	32.0	36.0
28	34.71520420172458	36.0	36.0	36.0	32.0	36.0
29	34.68707090303595	36.0	36.0	36.0	32.0	36.0
30	34.597558188347506	36.0	36.0	36.0	32.0	36.0
31	34.598437931540694	36.0	36.0	36.0	32.0	36.0
32	34.56767571137456	36.0	36.0	36.0	32.0	36.0
33	34.633223510577004	36.0	36.0	36.0	32.0	36.0
34	34.59294655675623	36.0	36.0	36.0	32.0	36.0
35	34.58511488735199	36.0	36.0	36.0	32.0	36.0
36	34.49653096820448	36.0	36.0	36.0	32.0	36.0
37	34.376540750237886	36.0	36.0	36.0	32.0	36.0
38	34.02377652603453	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	8.0
21	37.0
22	123.0
23	432.0
24	1275.0
25	3171.0
26	7099.0
27	14327.0
28	26310.0
29	43554.0
30	68285.0
31	99623.0
32	142492.0
33	228181.0
34	601978.0
35	1576424.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.517795954322516	21.424856569097987	13.013419352178277	27.043928124401223
2	9.957022323439094	20.47178405876898	50.887758631169355	18.683434986622572
3	19.67052462196813	26.14735396351856	30.336140099192377	23.845981315320934
4	10.040816587679242	17.739309150017576	41.20118706850312	31.01868719380006
5	9.185058644256126	42.048591005854654	37.024240763311944	11.742109586577278
6	30.174701251227642	39.727268558786655	18.516754421915483	11.581275768070217
7	26.524627573925375	31.59666016187338	22.89018984754613	18.988522416655115
8	20.557182770166996	48.18672998585271	19.17284573768511	12.083241506295188
9	26.219044016965803	14.5339380091199	21.501975694479004	37.74504227943529
10	15.80327097339024	29.6586097918772	35.63819029030413	18.899928944428428
11	33.55209182026683	18.574493769968733	31.9299588930266	15.943455516737831
12	28.175032257376543	21.982824502098953	35.413125464310724	14.429017776213785
13	32.44816168825745	23.68597188725756	25.812466787402048	18.053399637082943
14	17.042598409495398	28.37521918046323	26.35930986901246	28.22287254102891
15	18.027981876223865	45.042958126712165	20.82442778481375	16.10463221225022
16	16.10369538875285	27.497470068250397	38.9484014776863	17.45043306531045
17	16.820832034453232	34.27507916800109	32.223162589693814	16.680926207851858
18	17.381095154090133	26.235150556939647	42.08595464221822	14.297799646752004
19	25.43065650880223	24.303803227573393	27.402205436208664	22.863334827415713
20	24.899149439399203	29.976956060115427	30.293308157867514	14.830586342617853
21	19.63328723120272	27.103254199043903	26.347420964348515	26.916037605404863
22	22.853319210043647	35.59317816672117	26.92694041203987	14.626562211195312
23	16.399177057126142	34.886315731104695	31.540622140831577	17.17388507093759
24	24.97347440978118	26.920319437419334	32.79010820308098	15.316097949718499
25	19.68674022151764	31.884819359333456	32.48240512988213	15.946035289266774
26	13.954651443845705	34.74115991071048	34.49739098289565	16.806797662548163
27	16.600611804282345	33.51100017843699	31.879154791601945	18.009233225678717
28	15.309841998122502	34.87771214162906	27.93101107196797	21.881434788280473
29	19.337395199481325	28.97369336808704	27.062357974792068	24.626553457639567
30	15.559546884269515	30.239634936788228	32.113292439789134	22.087525739153122
31	23.453349262313118	26.75190637684075	29.98921914705076	19.80552521379537
32	17.880469381204634	33.13717133594069	30.627539480919523	18.35481980193515
33	15.563314673298922	33.70262405178791	35.10356621231633	15.63049506259684
34	18.386170650274178	32.84850893303679	33.812423111333544	14.952897305355487
35	16.346623794440802	34.63700018590129	32.72463398240016	16.291742037257748
36	14.663630634399702	31.98333926345412	35.605499692356474	17.74753040978971
37	16.15165848475876	31.70996128774498	28.1486542061855	23.989726021310755
38	18.4261234320577	36.42215019189065	27.69228964629347	17.459436729758178
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	27.0
1	67.5
2	108.0
3	108.0
4	473.0
5	838.0
6	838.0
7	1167.5
8	1497.0
9	1607.0
10	1717.0
11	1717.0
12	2337.5
13	2958.0
14	4297.0
15	5636.0
16	5636.0
17	8362.5
18	11089.0
19	11089.0
20	14700.5
21	18312.0
22	25505.5
23	32699.0
24	32699.0
25	55683.0
26	78667.0
27	78667.0
28	113953.5
29	149240.0
30	214463.0
31	279686.0
32	279686.0
33	350175.0
34	420664.0
35	420664.0
36	437572.0
37	454480.0
38	448608.0
39	442736.0
40	442736.0
41	367674.0
42	292612.0
43	226684.0
44	160756.0
45	160756.0
46	131365.0
47	101974.0
48	101974.0
49	90483.0
50	78992.0
51	71781.0
52	64570.0
53	64570.0
54	56650.0
55	48730.0
56	48730.0
57	45889.5
58	43049.0
59	37406.5
60	31764.0
61	31764.0
62	31750.5
63	31737.0
64	26109.0
65	20481.0
66	20481.0
67	17509.5
68	14538.0
69	14538.0
70	11825.5
71	9113.0
72	7080.0
73	5047.0
74	5047.0
75	3729.0
76	2411.0
77	2411.0
78	2662.0
79	2913.0
80	2306.5
81	1700.0
82	1700.0
83	1707.0
84	1714.0
85	1714.0
86	1072.5
87	431.0
88	382.0
89	333.0
90	333.0
91	195.0
92	57.0
93	41.5
94	26.0
95	26.0
96	16.5
97	7.0
98	7.0
99	9.5
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010450282779675694
2	0.0
3	0.0
4	1.4218071809082576E-4
5	7.109035904541288E-5
6	1.4218071809082576E-4
7	3.909969747497708E-4
8	0.002168255950885093
9	0.004727508876519956
10	0.001066355385681193
11	0.006149316057428214
12	3.909969747497708E-4
13	2.1327107713623862E-4
14	0.0
15	0.0
16	1.4218071809082576E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	7.109035904541288E-5
22	3.554517952270644E-5
23	1.0663553856811931E-4
24	0.0
25	3.554517952270644E-5
26	3.554517952270644E-5
27	1.0663553856811931E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2813321.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.30234861499487
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.03515397823433	29.50627323284141
2	13.00765733044762	9.964476514703966
3	3.9249615974646908	4.510057422196792
4	1.663335049084345	2.5483855565427276
5	0.9546750857606143	1.8283148974426586
6	0.5708381656926335	1.311866545506207
7	0.40815240148002674	1.0943236898674733
8	0.3037274317918653	0.9306779181143279
9	0.2290276989345314	0.789506889037246
>10	1.542723928687113	11.526785020853943
>50	0.17663967804835617	4.728184417099722
>100	0.153825288726385	11.850540186817026
>500	0.01678606947949978	4.365888313415323
>1k	0.010817689220122056	8.278428339418033
>5k	0.0011190712986333163	2.8340340229979875
>10k+	5.595356493166582E-4	3.932257033145149
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCA	27059	0.9618170127049136	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	20625	0.7331193276558203	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCA	19865	0.7061049912185634	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAG	19651	0.6984983228007042	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	12195	0.43347346427940503	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCA	10693	0.38008460463629995	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCA	9718	0.3454280546016612	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAA	9288	0.3301436274068974	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTA	6885	0.24472856101383383	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCAT	6824	0.24256030506294873	No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	6811	0.24209821772915355	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATG	6413	0.2279512362791164	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	6000	0.2132710771362386	No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCA	5906	0.20992983026110423	No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAAT	5635	0.2002970866104508	No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAG	5480	0.19478758378443128	No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTT	5257	0.18686100875086775	No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACG	5125	0.1821690450538705	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAA	4960	0.17630409043262393	No Hit
ACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAA	4833	0.17178985263324023	No Hit
TTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGT	4695	0.16688461785910674	No Hit
GACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAA	4624	0.16436091011299456	No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTT	4617	0.16411209385633563	No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAA	4524	0.16080639216072393	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAAT	4452	0.15824713923508907	No Hit
CCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	4370	0.15533243451422712	No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTC	4320	0.1535551755380918	No Hit
ACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT	4226	0.1502139286629574	No Hit
CACTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAA	4075	0.14484660655502873	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	4012	0.14260726024509823	No Hit
ATACACACTTTAGGACGTGAAATATGGCGAGGAAAACT	3641	0.12941999864217413	No Hit
GAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTT	3587	0.127500558947948	No Hit
CCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGT	3451	0.12266641453285991	No Hit
AGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCT	3393	0.12060479412054294	No Hit
ATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCAC	3388	0.12042706822292942	No Hit
GAAATACACACTTTAGGACGTGAAATATGGCGAGGAAA	3311	0.11769008939968102	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAA	3297	0.11719245688636314	No Hit
CTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGT	3145	0.11178958959891176	No Hit
ACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAA	2963	0.10532036692577917	No Hit
TCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAG	2861	0.10169475861446312	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	7.109035904541288E-5	0.0
17	0.0	0.0	0.0	1.4218071809082576E-4	0.0
18	0.0	0.0	0.0	1.777258976135322E-4	0.0
19	0.0	0.0	0.0	2.1327107713623862E-4	0.0
20	0.0	0.0	0.0	3.909969747497708E-4	0.0
21	0.0	0.0	0.0	5.331776928405965E-4	0.0
22	0.0	0.0	0.0	7.109035904541288E-4	0.0
23	0.0	0.0	0.0	9.597198471130739E-4	0.0
24	0.0	0.0	0.0	0.0015284427194763768	0.0
25	0.0	0.0	0.0	0.0019194396942261477	0.0
26	0.0	0.0	0.0	0.0024170722075440377	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCCAA	35	0.0020672455	22.857208	11
TAGGACC	6330	0.0	21.96687	4
TCGGACG	60	4.7215544E-6	21.33491	4
TCTAGCG	280	0.0	21.142166	28
AAGACGG	265	0.0	19.926708	5
CTAGCGG	300	0.0	19.732685	29
TTCATCG	50	7.2010694E-4	19.19937	28
TAGCGGC	310	0.0	19.096148	30
AGCGGCG	310	0.0	19.096148	31
AGGACCT	11455	0.0	18.984182	5
GCGCAAG	280	0.0	18.85753	1
GACGTGC	85	2.3963185E-7	18.825588	7
GGACCTG	11380	0.0	18.574966	6
GTCCTAG	810	0.0	18.568283	1
GCGGCGC	340	0.0	18.35234	32
CAAGACG	290	0.0	18.20824	4
GTGTAGG	625	0.0	18.176374	1
CTAGGAC	865	0.0	18.128185	3
TGTAGGA	20575	0.0	17.987247	2
ATACCCG	45	0.008847792	17.779722	6
>>END_MODULE