FastQCFastQC Report
Thu 2 Feb 2017
SRR4062774_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062774_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1997660
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT48740.24398546299170032No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT40420.20233673397875512No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA39740.19893275131904328No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC39350.19698046714656148No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG38100.19072314608091467No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG37180.1861177577765986No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG37140.18591752350249793No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC35480.17760780112731897No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC35400.17720733257911755No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA33630.1683469659501617No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT32500.16269034770681698No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG32430.16233993772714075No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG31190.1561326752300191No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA29340.14687184005286186No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT29180.14607090295645905No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT28430.14231651031707096No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG28010.14021405043901364No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA27980.14006387473343812No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT27700.13866223481473325No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC25980.13005216102840322No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT25930.12980186818577735No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG25540.12784958401329555No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC25370.12699858834836758No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT24230.1212919115364977No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG24170.12099156012534665No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT23880.1195398616381166No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG23680.1185386902676131No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC23560.117937987445311No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT22470.112481603476067No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG22130.11077961214621107No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATA21770.10897750367930478No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC21310.10667480952714675No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT21060.1054233453140174No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC20990.10507293533434119No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT20990.10507293533434119No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCT20970.10497281819729083No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTTA507.19833E-419.2000617
TTAGGAC2900.017.6525763
ACCGGTG550.001366011117.4528527
GTCCTAT9850.016.096181
TCTAGCG8350.015.90344428
ATCGTTT8050.015.90066929
CATCGTT7850.015.89734328
CTATTCC9600.015.829424
CGCTTCG8000.015.8000532
TAGGACG3850.015.7883044
ATAATAC2250.015.6421443
AGGCCCG8900.015.64049910
GGCATCG7800.015.58940226
CTAGCGG8550.015.53221229
AAACGCT8150.015.50925129
AAGACGG9600.015.4953935
CAAGACG9500.015.3225284
CGGCATC8100.015.20992425
TCTAGAT6650.015.1621182
CGAGCCG9400.015.14974315
Produced by FastQC (version 0.11.5)