##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062774_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1997660
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.263873231681067	32.0	32.0	32.0	32.0	32.0
2	30.918456093629548	32.0	32.0	32.0	32.0	32.0
3	30.967469439243917	32.0	32.0	32.0	32.0	32.0
4	31.02603145680446	32.0	32.0	32.0	32.0	32.0
5	30.973153089114263	32.0	32.0	32.0	32.0	32.0
6	34.62592633381056	36.0	36.0	36.0	32.0	36.0
7	34.53355876375359	36.0	36.0	36.0	32.0	36.0
8	34.53471411551515	36.0	36.0	36.0	32.0	36.0
9	34.63963537338686	36.0	36.0	36.0	32.0	36.0
10	34.389951242954254	36.0	36.0	36.0	32.0	36.0
11	34.62705114984532	36.0	36.0	36.0	32.0	36.0
12	34.447987144959605	36.0	36.0	36.0	32.0	36.0
13	34.536419610944805	36.0	36.0	36.0	32.0	36.0
14	34.435433957730545	36.0	36.0	36.0	32.0	36.0
15	34.392536267432895	36.0	36.0	36.0	32.0	36.0
16	34.40652463382157	36.0	36.0	36.0	32.0	36.0
17	34.32164382327323	36.0	36.0	36.0	32.0	36.0
18	34.350282830912164	36.0	36.0	36.0	32.0	36.0
19	34.343135969083825	36.0	36.0	36.0	32.0	36.0
20	34.322742108266674	36.0	36.0	36.0	32.0	36.0
21	34.296532943543944	36.0	36.0	36.0	32.0	36.0
22	34.27402110469249	36.0	36.0	36.0	32.0	36.0
23	34.22137500876025	36.0	36.0	36.0	32.0	36.0
24	34.20489823093019	36.0	36.0	36.0	32.0	36.0
25	34.17989647888029	36.0	36.0	36.0	32.0	36.0
26	34.129188650721346	36.0	36.0	36.0	32.0	36.0
27	34.138221218826025	36.0	36.0	36.0	32.0	36.0
28	34.1192755523963	36.0	36.0	36.0	32.0	36.0
29	34.07891132625171	36.0	36.0	36.0	32.0	36.0
30	34.05219757115825	36.0	36.0	36.0	32.0	36.0
31	34.07851185887488	36.0	36.0	36.0	32.0	36.0
32	34.026841404443196	36.0	36.0	36.0	32.0	36.0
33	33.996787241072056	36.0	36.0	36.0	32.0	36.0
34	34.00826967552036	36.0	36.0	36.0	32.0	36.0
35	33.96058288197191	36.0	36.0	36.0	32.0	36.0
36	33.90764944985633	36.0	36.0	36.0	32.0	36.0
37	33.920271717909955	36.0	36.0	36.0	32.0	36.0
38	33.30912667821351	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	9.0
8	4.0
9	5.0
10	20.0
11	13.0
12	11.0
13	10.0
14	288.0
15	632.0
16	792.0
17	871.0
18	1053.0
19	1359.0
20	1805.0
21	2714.0
22	3887.0
23	5844.0
24	8613.0
25	12861.0
26	18428.0
27	26656.0
28	37173.0
29	49572.0
30	67332.0
31	90377.0
32	123113.0
33	185416.0
34	421583.0
35	937218.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.20682104212204	17.673902943044208	11.407447652566512	25.711828362267248
2	16.746715365393218	20.123135918833675	37.60153429037818	25.52861442539493
3	18.38065699917143	24.330697426423654	29.018105901878176	28.27053967252674
4	12.047581192593498	15.490669785081668	36.91607311989011	35.54567590243472
5	14.460247409964477	36.70977385495801	33.58000576675864	15.24997296831887
6	33.61518060685184	36.49120418250608	16.942773873753914	12.95084133688817
7	30.055129529113827	30.82217735859824	21.245818230238495	17.87687488204944
8	28.02155334673576	32.7031589148839	19.887187332302787	19.388100406077548
9	27.527486087381803	14.048878505561794	19.08415250542263	39.33948290163377
10	16.00537033162098	25.898834726142123	31.20819446126984	26.887600480967063
11	37.95339044140616	20.951223858839736	22.51719049106535	18.578195208688758
12	25.061823941433953	24.087508660357788	28.46484395336823	22.385823444840028
13	29.103382878931704	18.953494426620846	25.798757555002823	26.144365139444623
14	23.62963600809427	20.631030778057355	24.86985985215238	30.86947336169599
15	25.07722173013728	27.639045429760394	22.408596960400487	24.875135879701837
16	25.897209194279007	25.75822963268543	23.978780584515196	24.36578058852037
17	23.771673768168913	26.053634283482623	25.400830149757414	24.77386179859105
18	25.30615064684368	24.17541323203571	26.77740587701109	23.741030244109524
19	25.5746461809571	24.900500127660212	25.614095690091066	23.910758001291622
20	25.655483210798913	24.01771384116616	25.00653305525713	25.320269892777798
21	27.760083703373667	24.129349746937468	23.96744980050362	24.143116749185246
22	25.816630429830784	24.33248043686455	25.054280160219715	24.79660897308495
23	23.72329720393502	23.93681944479211	25.957495807154125	26.382387544118753
24	24.721931280878444	25.375698175781086	25.18344324574386	24.71892729759661
25	24.64400566166306	24.60655497215481	25.452399823160647	25.297039543021484
26	24.318090101529197	25.93520716902874	26.08205850220652	23.66464422723554
27	25.313071034859096	24.778144352606045	24.85579257761068	25.052992034924177
28	23.903859314323533	24.79776283822379	26.222822073850168	25.075555773602513
29	23.88277966492026	25.08061588987877	25.9860771439371	25.05052730126387
30	23.803888470052534	25.40976713032352	26.392197028631387	24.39414737099256
31	25.027971891440526	25.00854800977802	24.512988719630783	25.450491379150662
32	24.21746598401916	25.147770957643917	24.544872459158046	26.089890599178883
33	23.974015142147245	24.440505018596014	25.791413242044563	25.794066597212172
34	25.153750182737927	24.749924401570844	25.872586107111463	24.22373930857977
35	26.278916305682227	24.49992890458996	25.565358711390683	23.65579607833713
36	23.987901885311235	25.64896442060825	25.129422390624313	25.233711303456204
37	25.59724689943263	25.369056755664825	24.888896231558427	24.144800113344118
38	24.248947450100875	24.97659609617878	25.739037711573793	25.035418742146554
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	782.0
1	594.0
2	406.0
3	406.0
4	867.0
5	1328.0
6	1328.0
7	1544.5
8	1761.0
9	1685.5
10	1610.0
11	1610.0
12	2158.5
13	2707.0
14	3620.0
15	4533.0
16	4533.0
17	6648.5
18	8764.0
19	8764.0
20	10826.5
21	12889.0
22	14538.5
23	16188.0
24	16188.0
25	19338.5
26	22489.0
27	22489.0
28	28732.5
29	34976.0
30	42590.0
31	50204.0
32	50204.0
33	65183.0
34	80162.0
35	80162.0
36	87640.0
37	95118.0
38	108205.5
39	121293.0
40	121293.0
41	124152.5
42	127012.0
43	142821.0
44	158630.0
45	158630.0
46	157614.0
47	156598.0
48	156598.0
49	166903.5
50	177209.0
51	179820.5
52	182432.0
53	182432.0
54	168269.0
55	154106.0
56	154106.0
57	150509.5
58	146913.0
59	129733.0
60	112553.0
61	112553.0
62	112810.5
63	113068.0
64	93255.5
65	73443.0
66	73443.0
67	62273.0
68	51103.0
69	51103.0
70	41690.0
71	32277.0
72	25419.0
73	18561.0
74	18561.0
75	13733.5
76	8906.0
77	8906.0
78	9791.0
79	10676.0
80	8254.5
81	5833.0
82	5833.0
83	6294.5
84	6756.0
85	6756.0
86	4407.0
87	2058.0
88	1863.5
89	1669.0
90	1669.0
91	1208.5
92	748.0
93	619.5
94	491.0
95	491.0
96	443.5
97	396.0
98	396.0
99	704.0
100	1012.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12419530851095784
2	0.05841834946887858
3	0.01226434928866774
4	0.0032037483856111647
5	2.002342741006978E-4
6	3.003514111510467E-4
7	5.005856852517445E-5
8	6.007028223020934E-4
9	6.507613908272679E-4
10	9.010542334531402E-4
11	0.002202577015107676
12	0.0026030455633090717
13	0.01541803910575373
14	0.009911596567984541
15	0.02447864000881031
16	0.012214290720142566
17	0.021525184465825017
18	0.005706676811869888
19	0.008509956649279657
20	0.0063073796341719815
21	0.006507613908272679
22	0.007658960984351692
23	0.010512299390286636
24	0.01561827337985443
25	0.018371494648739026
26	0.02012354454712013
27	0.009511128019783146
28	0.006457555339747505
29	0.011263177918164252
30	0.0031036312485608164
31	0.00595696965449576
32	0.007158375299099946
33	0.009461069451257973
34	0.013215462090646054
35	0.01722014757266001
36	0.016218976202156524
37	0.010161889410610414
38	0.006507613908272679
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1997660.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.12440660096262
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.43448405571391	34.16750659410078
2	13.340234589514202	11.772598703599007
3	4.254574321571688	5.631917018371315
4	1.6671683390225882	2.942512546531367
5	0.8715368985946038	1.9228024240665114
6	0.5005526863562495	1.325195415479236
7	0.33259080605735514	1.0272760370751648
8	0.2346647540963159	0.8283554419728602
9	0.18217369850789014	0.7234475710466991
>10	0.9411492677020998	7.584679789062658
>50	0.09298041773191656	2.8660425375681164
>100	0.11100388558572993	10.779269544874664
>500	0.022154607528873965	6.753823500898913
>1k	0.014731672016622382	11.674572875352645
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4874	0.24398546299170032	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	4042	0.20233673397875512	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	3974	0.19893275131904328	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3935	0.19698046714656148	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	3810	0.19072314608091467	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	3718	0.1861177577765986	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	3714	0.18591752350249793	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	3548	0.17760780112731897	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	3540	0.17720733257911755	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	3363	0.1683469659501617	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3250	0.16269034770681698	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	3243	0.16233993772714075	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	3119	0.1561326752300191	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	2934	0.14687184005286186	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	2918	0.14607090295645905	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	2843	0.14231651031707096	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2801	0.14021405043901364	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	2798	0.14006387473343812	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	2770	0.13866223481473325	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2598	0.13005216102840322	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	2593	0.12980186818577735	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	2554	0.12784958401329555	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	2537	0.12699858834836758	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2423	0.1212919115364977	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	2417	0.12099156012534665	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	2388	0.1195398616381166	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	2368	0.1185386902676131	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	2356	0.117937987445311	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT	2247	0.112481603476067	No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG	2213	0.11077961214621107	No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATA	2177	0.10897750367930478	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	2131	0.10667480952714675	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	2106	0.1054233453140174	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	2099	0.10507293533434119	No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT	2099	0.10507293533434119	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCT	2097	0.10497281819729083	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	5.0058568525174455E-5	0.0
8	0.0	0.0	0.0	5.0058568525174455E-5	0.0
9	0.0	0.0	0.0	1.0011713705034891E-4	0.0
10	0.0	0.0	0.0	1.5017570557552336E-4	0.0
11	0.0	0.0	0.0	1.5017570557552336E-4	0.0
12	0.0	0.0	0.0	1.5017570557552336E-4	0.0
13	0.0	5.0058568525174455E-5	0.0	2.0023427410069782E-4	0.0
14	0.0	5.0058568525174455E-5	0.0	2.0023427410069782E-4	0.0
15	0.0	5.0058568525174455E-5	0.0	2.0023427410069782E-4	0.0
16	0.0	5.0058568525174455E-5	0.0	2.502928426258723E-4	0.0
17	0.0	5.0058568525174455E-5	0.0	2.502928426258723E-4	0.0
18	0.0	1.0011713705034891E-4	0.0	3.5040997967622115E-4	0.0
19	0.0	1.0011713705034891E-4	0.0	5.005856852517446E-4	0.0
20	0.0	1.0011713705034891E-4	0.0	5.005856852517446E-4	0.0
21	0.0	1.0011713705034891E-4	0.0	6.007028223020934E-4	0.0
22	0.0	1.0011713705034891E-4	0.0	8.509956649279657E-4	0.0
23	0.0	1.5017570557552336E-4	0.0	0.0014516984872300592	0.0
24	0.0	1.5017570557552336E-4	0.0	0.0021525184465825014	0.0
25	0.0	1.5017570557552336E-4	0.0	0.0028032798374097692	0.0
26	0.0	2.0023427410069782E-4	0.0	0.0038044512079132586	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCTTA	50	7.19833E-4	19.20006	17
TTAGGAC	290	0.0	17.652576	3
ACCGGTG	55	0.0013660111	17.452852	7
GTCCTAT	985	0.0	16.09618	1
TCTAGCG	835	0.0	15.903444	28
ATCGTTT	805	0.0	15.900669	29
CATCGTT	785	0.0	15.897343	28
CTATTCC	960	0.0	15.82942	4
CGCTTCG	800	0.0	15.80005	32
TAGGACG	385	0.0	15.788304	4
ATAATAC	225	0.0	15.642144	3
AGGCCCG	890	0.0	15.640499	10
GGCATCG	780	0.0	15.589402	26
CTAGCGG	855	0.0	15.532212	29
AAACGCT	815	0.0	15.509251	29
AAGACGG	960	0.0	15.495393	5
CAAGACG	950	0.0	15.322528	4
CGGCATC	810	0.0	15.209924	25
TCTAGAT	665	0.0	15.162118	2
CGAGCCG	940	0.0	15.149743	15
>>END_MODULE