FastQCFastQC Report
Thu 2 Feb 2017
SRR4062774_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062774_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1997660
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT55190.2762732396904378No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC43580.21815524163271027No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT41020.2053402480902656No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG40890.20468948669943834No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT40250.20148573831382716No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA39370.1970805842836118No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC37850.1894716818677853No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG36690.18366488791886507No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC36550.18296406795951262No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA36340.18191283802048397No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA33400.16719561887408269No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG33260.16649479891473024No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG32360.16198952774746453No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG31820.1592863650471051No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG31690.15863560365627785No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC30460.1524783997276814No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT29480.14757266001221428No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG29000.14516984872300592No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT27820.13926293763703534No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT27260.13645965779962557No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA26820.1342570807845179No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT25620.12825005256149696No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG25360.12694852977984242No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC25090.1255969484296627No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT24850.12439554278505852No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT24580.1230439614348788No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT24390.12209284863290049No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC24370.12199273149585016No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG24310.12169238008469908No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG23640.1183384559935124No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT23250.11638617182103062No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT22820.1142336533744481No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATA21510.10767598089765026No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGACCCCCG21380.10702521950682299No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC20830.1042719982379384No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT20430.1022696554969314No Hit
AAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGC20240.10131854269495308No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT400.00447958520.0038818
TCTAGCG7350.018.28468328
TATACCG450.00884260817.7812275
TACGATC450.00884898617.7790034
CTAGCGG7650.017.358529
GTCCTAC4250.017.3175371
CGGTCCA8050.016.89599610
AACCGCG5050.016.7953387
GTCTAAA1456.91216E-1116.551621
TAGCGGC8100.016.39413830
TACGACT600.002441631216.00114
CGCGTAA4250.015.81324810
AGCGGCG8300.015.80633831
ACCGTCG7700.015.7952728
CGGTCCT5300.015.69722912
CCGGTCC9350.015.5744349
TTTCGGA8750.015.54198230
TTCGGAA8700.015.44740631
GTCGCGT4250.015.4382898
TCGCGTA4250.015.4375159
Produced by FastQC (version 0.11.5)