##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062774_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1997660
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.211269685532073	32.0	32.0	32.0	32.0	32.0
2	31.311136529739795	32.0	32.0	32.0	32.0	32.0
3	31.393451338065535	32.0	32.0	32.0	32.0	32.0
4	31.483332999609544	32.0	32.0	32.0	32.0	32.0
5	31.42056656287857	32.0	32.0	32.0	32.0	32.0
6	34.941956589209376	36.0	36.0	36.0	36.0	36.0
7	34.961853368441076	36.0	36.0	36.0	36.0	36.0
8	34.90449075418239	36.0	36.0	36.0	36.0	36.0
9	34.99833104732537	36.0	36.0	36.0	36.0	36.0
10	34.85308460899252	36.0	36.0	36.0	32.0	36.0
11	35.02971827037634	36.0	36.0	36.0	36.0	36.0
12	34.908307219446755	36.0	36.0	36.0	32.0	36.0
13	34.97453921087673	36.0	36.0	36.0	36.0	36.0
14	34.919104852677634	36.0	36.0	36.0	32.0	36.0
15	34.89054593874834	36.0	36.0	36.0	32.0	36.0
16	34.89835857953806	36.0	36.0	36.0	32.0	36.0
17	34.85261756254818	36.0	36.0	36.0	32.0	36.0
18	34.86204359100147	36.0	36.0	36.0	32.0	36.0
19	34.8559399497412	36.0	36.0	36.0	32.0	36.0
20	34.83749937426789	36.0	36.0	36.0	32.0	36.0
21	34.82691148643914	36.0	36.0	36.0	32.0	36.0
22	34.814142546779735	36.0	36.0	36.0	32.0	36.0
23	34.75506993182023	36.0	36.0	36.0	32.0	36.0
24	34.726832393900864	36.0	36.0	36.0	32.0	36.0
25	34.701621897620214	36.0	36.0	36.0	32.0	36.0
26	34.64091987625522	36.0	36.0	36.0	32.0	36.0
27	34.62641140133957	36.0	36.0	36.0	32.0	36.0
28	34.592709470079996	36.0	36.0	36.0	32.0	36.0
29	34.55068079653194	36.0	36.0	36.0	32.0	36.0
30	34.52330476657689	36.0	36.0	36.0	32.0	36.0
31	34.53476217174094	36.0	36.0	36.0	32.0	36.0
32	34.49870298248951	36.0	36.0	36.0	32.0	36.0
33	34.4633936705946	36.0	36.0	36.0	32.0	36.0
34	34.45593043861318	36.0	36.0	36.0	32.0	36.0
35	34.41351030705926	36.0	36.0	36.0	32.0	36.0
36	34.385008960483766	36.0	36.0	36.0	32.0	36.0
37	34.379157113823176	36.0	36.0	36.0	32.0	36.0
38	33.920230669883765	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	11.0
21	44.0
22	173.0
23	549.0
24	1343.0
25	3333.0
26	7082.0
27	13628.0
28	23488.0
29	36961.0
30	55738.0
31	80110.0
32	114704.0
33	184032.0
34	448681.0
35	1027782.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.66801235752723	18.880873817952256	12.07372062997156	26.377393194548947
2	15.733558263167907	21.121161759258335	36.91293813762101	26.232341839952745
3	17.431194497562146	24.97251784587968	29.468828529379376	28.1274591271788
4	11.53892361848086	16.29299539059778	37.04136247682284	35.12671851409853
5	13.88420145780636	37.24094052087969	33.36790713530187	15.506950886012078
6	33.59307107930495	36.96757599907091	16.577328629153367	12.862024292470775
7	29.616579097018093	31.126805122824443	21.187967049249917	18.068648730907547
8	27.805599886264197	32.98543460892305	19.710133899876453	19.498831604936296
9	27.47404854683877	13.857695526978697	18.87998253883707	39.78827338734546
10	15.817380326165202	26.238237358320177	31.116148170989938	26.828234144524682
11	38.03466211446908	20.952206931421678	22.41798785524412	18.595143098865123
12	24.846006480617966	24.304670139749277	28.322242796407178	22.52708058322558
13	29.115202132100954	18.90742841352907	25.755611332092542	26.221758122277432
14	23.49043380755484	20.593544447002994	24.86153799945937	31.054483745982797
15	25.015017570557553	27.75397214741247	22.2744110609413	24.956599221088673
16	25.797495969031754	25.86402383990461	23.739637245395738	24.598842945667904
17	23.75804691489042	26.265831022296087	25.177557742558793	24.798564320254698
18	25.305607560846187	24.249071413553857	26.69488301312536	23.750438012474596
19	25.615319924311446	24.89572800176206	25.54018201295516	23.948770060971334
20	25.691759358449385	23.96438833435119	24.980977743960434	25.36287456323899
21	27.658652110947973	24.142132508233395	24.018237365073183	24.180978015745445
22	25.70767798324039	24.413413694022008	25.06297367920467	24.815934643532934
23	23.57487975907764	23.82537333755161	26.048779169186286	26.550967734184468
24	24.67216643472863	25.345053712844027	25.225463792637388	24.757316059789954
25	24.65781196890961	24.577968512143464	25.533737239438764	25.230482279508166
26	24.307826618884025	25.802828013017248	26.110188085341978	23.77915728275675
27	25.418915550109176	24.59755373542418	24.915425963803614	25.06810475066303
28	23.892253937106414	24.703052571508664	26.32760329585615	25.07709019552877
29	23.9673918484627	24.7629726780333	26.160207442707968	25.109428030796032
30	23.885395913218467	25.148623889951242	26.503609222790665	24.462370974039626
31	25.019673017430392	24.874753461550014	24.61119509826497	25.49437842275462
32	24.351190893345215	25.01847161178579	24.683129261235646	25.94720823363335
33	23.984411761761258	24.26343822272058	25.869917803830482	25.882232211687672
34	25.074336974259886	24.537959412512638	26.052781754652944	24.334921858574532
35	26.286505211096983	24.35609663306068	25.615369982879972	23.742028172962364
36	24.191954586866636	25.438863470260202	25.11538500045053	25.253796942422635
37	25.74832554088283	25.158585545087753	24.84752160027232	24.245567313757096
38	24.382201558024448	24.681451980268896	25.875199859034932	25.06114660267173
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	411.0
1	326.0
2	241.0
3	241.0
4	769.0
5	1297.0
6	1297.0
7	1491.5
8	1686.0
9	1682.5
10	1679.0
11	1679.0
12	2192.5
13	2706.0
14	3692.0
15	4678.0
16	4678.0
17	7118.5
18	9559.0
19	9559.0
20	11340.5
21	13122.0
22	14686.0
23	16250.0
24	16250.0
25	19228.5
26	22207.0
27	22207.0
28	28613.5
29	35020.0
30	42497.5
31	49975.0
32	49975.0
33	65427.0
34	80879.0
35	80879.0
36	88223.0
37	95567.0
38	108289.0
39	121011.0
40	121011.0
41	123837.5
42	126664.0
43	142834.0
44	159004.0
45	159004.0
46	157494.0
47	155984.0
48	155984.0
49	166707.0
50	177430.0
51	179456.0
52	181482.0
53	181482.0
54	168136.5
55	154791.0
56	154791.0
57	151686.5
58	148582.0
59	131003.5
60	113425.0
61	113425.0
62	113487.5
63	113550.0
64	93537.5
65	73525.0
66	73525.0
67	62653.5
68	51782.0
69	51782.0
70	42364.5
71	32947.0
72	25709.0
73	18471.0
74	18471.0
75	13526.0
76	8581.0
77	8581.0
78	9625.0
79	10669.0
80	8167.0
81	5665.0
82	5665.0
83	5818.0
84	5971.0
85	5971.0
86	3730.5
87	1490.0
88	1266.5
89	1043.0
90	1043.0
91	618.5
92	194.0
93	128.5
94	63.0
95	63.0
96	42.0
97	21.0
98	21.0
99	29.5
100	38.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008660132354855181
2	0.0
3	0.0
4	2.002342741006978E-4
5	5.005856852517445E-5
6	2.002342741006978E-4
7	6.007028223020934E-4
8	0.0024028112892083738
9	0.004204919756114654
10	0.001101288507553838
11	0.004505271167265701
12	5.50644253776919E-4
13	3.003514111510467E-4
14	0.0
15	0.0
16	5.005856852517445E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	1.5017570557552336E-4
22	0.0
23	2.002342741006978E-4
24	0.0
25	5.005856852517445E-5
26	1.5017570557552336E-4
27	1.001171370503489E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.001171370503489E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1997660.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.17122382670103
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.842668122667	31.98378133015194
2	14.269957774056463	12.03563166574615
3	4.498198296816253	5.690835815757707
4	1.8693503717624922	3.1533117175249132
5	0.9219405575852482	1.9439680804420527
6	0.5400365774826373	1.3664402030175522
7	0.36535770968532716	1.078530722636555
8	0.2567057945099451	0.8660478014312011
9	0.17709105351620621	0.67213318099844
>10	1.0036432950017014	7.777961578374421
>50	0.09827855944162137	2.923942516315209
>100	0.11590654751692801	10.76895839675972
>500	0.02509275260593436	7.321832607395293
>1k	0.0156530980541781	12.138521425077661
>5k	1.1948929812349695E-4	0.27810295837122856
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5519	0.2762732396904378	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	4358	0.21815524163271027	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	4102	0.2053402480902656	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	4089	0.20468948669943834	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	4025	0.20148573831382716	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	3937	0.1970805842836118	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	3785	0.1894716818677853	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	3669	0.18366488791886507	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	3655	0.18296406795951262	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	3634	0.18191283802048397	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	3340	0.16719561887408269	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	3326	0.16649479891473024	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	3236	0.16198952774746453	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	3182	0.1592863650471051	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	3169	0.15863560365627785	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	3046	0.1524783997276814	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	2948	0.14757266001221428	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	2900	0.14516984872300592	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	2782	0.13926293763703534	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	2726	0.13645965779962557	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	2682	0.1342570807845179	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	2562	0.12825005256149696	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	2536	0.12694852977984242	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	2509	0.1255969484296627	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2485	0.12439554278505852	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT	2458	0.1230439614348788	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	2439	0.12209284863290049	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	2437	0.12199273149585016	No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG	2431	0.12169238008469908	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	2364	0.1183384559935124	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	2325	0.11638617182103062	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	2282	0.1142336533744481	No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATA	2151	0.10767598089765026	No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGACCCCCG	2138	0.10702521950682299	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	2083	0.1042719982379384	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT	2043	0.1022696554969314	No Hit
AAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGC	2024	0.10131854269495308	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	5.0058568525174455E-5	0.0
11	0.0	0.0	0.0	5.0058568525174455E-5	0.0
12	0.0	0.0	0.0	5.0058568525174455E-5	0.0
13	0.0	0.0	0.0	5.0058568525174455E-5	0.0
14	0.0	0.0	0.0	5.0058568525174455E-5	0.0
15	0.0	0.0	0.0	5.0058568525174455E-5	0.0
16	0.0	0.0	0.0	1.5017570557552336E-4	0.0
17	0.0	0.0	0.0	1.5017570557552336E-4	0.0
18	0.0	0.0	0.0	1.5017570557552336E-4	0.0
19	0.0	0.0	0.0	2.502928426258723E-4	0.0
20	0.0	0.0	0.0	3.5040997967622115E-4	0.0
21	0.0	0.0	0.0	4.505271167265701E-4	0.0
22	0.0	0.0	0.0	7.008199593524423E-4	0.0
23	0.0	0.0	0.0	0.0013015227816545359	0.0
24	0.0	0.0	0.0	0.0020023427410069783	0.0
25	0.0	0.0	0.0	0.002653104131834246	0.0
26	0.0	0.0	0.0	0.003854509776438433	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGCGT	40	0.004479585	20.003881	8
TCTAGCG	735	0.0	18.284683	28
TATACCG	45	0.008842608	17.781227	5
TACGATC	45	0.008848986	17.779003	4
CTAGCGG	765	0.0	17.3585	29
GTCCTAC	425	0.0	17.317537	1
CGGTCCA	805	0.0	16.895996	10
AACCGCG	505	0.0	16.795338	7
GTCTAAA	145	6.91216E-11	16.55162	1
TAGCGGC	810	0.0	16.394138	30
TACGACT	60	0.0024416312	16.0011	4
CGCGTAA	425	0.0	15.813248	10
AGCGGCG	830	0.0	15.806338	31
ACCGTCG	770	0.0	15.795272	8
CGGTCCT	530	0.0	15.697229	12
CCGGTCC	935	0.0	15.574434	9
TTTCGGA	875	0.0	15.541982	30
TTCGGAA	870	0.0	15.447406	31
GTCGCGT	425	0.0	15.438289	8
TCGCGTA	425	0.0	15.437515	9
>>END_MODULE