FastQCFastQC Report
Thu 2 Feb 2017
SRR4062773_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062773_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1543928
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT58120.3764424247762849No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA45330.29360177417599786No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT39980.2589498992180983No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC35890.23245902658673204No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT22770.14748097061521004No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT18020.11671528724137394No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17920.11606758864403004No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG17690.11457788187013902No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC17070.11056215056660673No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC16240.10518625220865221No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA16050.10395562487369876No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG15780.1022068386608702No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC15720.10181821950246385No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG15720.10181821950246385No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG15650.1013648304843231No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGCAA507.195263E-419.20087413
CATCGTT3500.019.19900928
TAGTACG507.20782E-419.19594
TAATACC904.378926E-717.7739814
ATCGTTT4000.017.19911229
CGTCGTA3000.017.0663410
CGTTTAT4350.016.91921631
ACCGTCG3200.016.4986158
ATACCGT3500.016.4541636
CGGCATC4050.016.1972225
GTATAGA1001.3011922E-616.0162941
CCGTCGT3200.015.9996939
TACCGTC3350.015.759877
TATACTG1950.015.585915
TCTAGCG3800.015.57814328
CTAGCGG3900.015.17870329
GTCGTAG3400.015.05902311
TAGCGGC3950.014.98705430
CGCTTCG3950.014.98705432
GGCGTTA1951.8189894E-1214.76894731
Produced by FastQC (version 0.11.5)