##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062773_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1543928
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.244227062401873	32.0	32.0	32.0	32.0	32.0
2	30.931107538693514	32.0	32.0	32.0	32.0	32.0
3	30.98626619894192	32.0	32.0	32.0	32.0	32.0
4	31.02912830131975	32.0	32.0	32.0	32.0	32.0
5	30.996906591499087	32.0	32.0	32.0	32.0	32.0
6	34.6340716665544	36.0	36.0	36.0	32.0	36.0
7	34.563054753848625	36.0	36.0	36.0	32.0	36.0
8	34.54865965252266	36.0	36.0	36.0	32.0	36.0
9	34.64831455870999	36.0	36.0	36.0	32.0	36.0
10	34.423608484333464	36.0	36.0	36.0	32.0	36.0
11	34.647162950603914	36.0	36.0	36.0	32.0	36.0
12	34.49868841034038	36.0	36.0	36.0	32.0	36.0
13	34.56077809327896	36.0	36.0	36.0	32.0	36.0
14	34.466712825986704	36.0	36.0	36.0	32.0	36.0
15	34.43417827774352	36.0	36.0	36.0	32.0	36.0
16	34.43186987994259	36.0	36.0	36.0	32.0	36.0
17	34.3741463332487	36.0	36.0	36.0	32.0	36.0
18	34.39465765242939	36.0	36.0	36.0	32.0	36.0
19	34.38456909907716	36.0	36.0	36.0	32.0	36.0
20	34.354297609733095	36.0	36.0	36.0	32.0	36.0
21	34.329503707426774	36.0	36.0	36.0	32.0	36.0
22	34.3075752237151	36.0	36.0	36.0	32.0	36.0
23	34.25539662471307	36.0	36.0	36.0	32.0	36.0
24	34.24469146229617	36.0	36.0	36.0	32.0	36.0
25	34.20619743925882	36.0	36.0	36.0	32.0	36.0
26	34.16954547103233	36.0	36.0	36.0	32.0	36.0
27	34.151371696089456	36.0	36.0	36.0	32.0	36.0
28	34.13839570239027	36.0	36.0	36.0	32.0	36.0
29	34.10494789912483	36.0	36.0	36.0	32.0	36.0
30	34.05852993144758	36.0	36.0	36.0	32.0	36.0
31	34.085638060842214	36.0	36.0	36.0	32.0	36.0
32	34.026142410785994	36.0	36.0	36.0	32.0	36.0
33	33.99172176422735	36.0	36.0	36.0	32.0	36.0
34	33.9897838500241	36.0	36.0	36.0	32.0	36.0
35	33.91973459902275	36.0	36.0	36.0	32.0	36.0
36	33.87934346679379	36.0	36.0	36.0	32.0	36.0
37	33.86501183993036	36.0	36.0	36.0	32.0	36.0
38	33.26712644631097	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	3.0
8	4.0
9	7.0
10	9.0
11	17.0
12	6.0
13	15.0
14	185.0
15	430.0
16	588.0
17	734.0
18	910.0
19	1203.0
20	1527.0
21	2089.0
22	3001.0
23	4466.0
24	6401.0
25	9549.0
26	13988.0
27	19975.0
28	28256.0
29	38631.0
30	52184.0
31	69629.0
32	94639.0
33	140910.0
34	319273.0
35	735298.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.51544483044181	17.25736801386737	11.588697288067666	25.63848986762315
2	16.12552639662795	19.856218706214623	38.067176569857644	25.951078327299783
3	18.826887187536233	24.184852491256617	28.731216870209273	28.25704345099787
4	12.450344680622297	15.470696479613549	36.15282924399554	35.92612959576862
5	14.227125169373622	36.81606089418909	33.81999373026133	15.136820206175953
6	33.87140023913125	35.60704491548148	17.05422942350715	13.467325421880123
7	29.889625610537284	30.91881934832411	20.94380109940431	18.24775394173429
8	28.155650451026965	33.18511763231409	19.63999453339905	19.019237383259895
9	27.477100527099168	14.417762037201362	18.875046796405517	39.230090639293955
10	15.876705727513674	26.74219322812899	31.875050197031445	25.506050847325888
11	37.09165831658459	21.36029799915409	22.35388759742392	19.1941560868374
12	24.74162700499776	24.05381982677545	28.872744984752817	22.33180818347397
13	29.707093184078154	19.63489487680293	25.403374436811095	25.25463750230782
14	23.577163096862122	20.227920781561124	25.29016402574975	30.904752095827003
15	25.067572470340675	27.41660663104265	23.193165268138966	24.322655630477712
16	25.339998458306585	25.928821699270028	24.625766879113748	24.10541296330964
17	23.486330181143483	26.091761829457827	26.087032572916392	24.3348754164823
18	24.587910664317548	24.561353508135557	27.550199502539126	23.300536325007773
19	25.099770112559987	25.74447839388418	25.9626415096784	23.193109983877434
20	25.247615500461517	24.39654753615209	26.3785079267404	23.97732903664599
21	26.31584061126178	24.529236836088074	24.793256739924203	24.361665812725942
22	25.05637024221229	25.103137625007204	25.55461776085285	24.28587437192765
23	23.58405990490873	24.80016582673697	26.112334659083547	25.50343960927075
24	24.0862994309109	25.771995115615454	25.916324144341985	24.22538130913166
25	24.485782121005016	24.854193456439425	25.889398861792305	24.770625560763254
26	23.635595657953722	25.642651871853143	26.59321950448431	24.12853296570882
27	24.78389041940643	25.640814703159847	25.57500208903229	24.000292788401435
28	23.766995070700787	25.007092749849402	26.400963836691865	24.824948342757946
29	23.705790025658448	25.572294834431425	26.4964135823302	24.225501557579932
30	24.067023517432748	25.37723236840264	26.657127904054473	23.898616210110138
31	24.30123323403464	25.27219694952867	25.48750604828132	24.93906376815537
32	23.61266209791297	25.829759946107707	25.588281017217035	24.96929693876229
33	23.47229869347515	25.24209899014762	26.218268028682658	25.06733428769457
34	24.15012278757264	25.570131429019327	26.512075029004556	23.76767075440348
35	25.09961785354054	25.371052616235946	26.11565193900945	23.413677591214068
36	23.782862154989076	26.02956695124582	25.883163836098472	24.304407057666634
37	24.828211193996978	25.89897626308821	25.497747053290805	23.77506548962401
38	23.892419822051334	25.945255134031513	25.788176829363085	24.37414821455407
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	150.0
1	220.5
2	291.0
3	291.0
4	791.5
5	1292.0
6	1292.0
7	1646.0
8	2000.0
9	2032.0
10	2064.0
11	2064.0
12	2692.5
13	3321.0
14	4080.5
15	4840.0
16	4840.0
17	7033.5
18	9227.0
19	9227.0
20	10812.0
21	12397.0
22	13021.5
23	13646.0
24	13646.0
25	16018.5
26	18391.0
27	18391.0
28	23571.0
29	28751.0
30	35203.0
31	41655.0
32	41655.0
33	51337.5
34	61020.0
35	61020.0
36	66759.0
37	72498.0
38	81098.5
39	89699.0
40	89699.0
41	94411.5
42	99124.0
43	110479.5
44	121835.0
45	121835.0
46	130762.5
47	139690.0
48	139690.0
49	140896.5
50	142103.0
51	138847.5
52	135592.0
53	135592.0
54	128606.0
55	121620.0
56	121620.0
57	117646.5
58	113673.0
59	101535.5
60	89398.0
61	89398.0
62	84069.0
63	78740.0
64	64941.0
65	51142.0
66	51142.0
67	42658.0
68	34174.0
69	34174.0
70	27763.5
71	21353.0
72	16799.0
73	12245.0
74	12245.0
75	9286.0
76	6327.0
77	6327.0
78	6044.5
79	5762.0
80	4492.5
81	3223.0
82	3223.0
83	3228.0
84	3233.0
85	3233.0
86	2193.5
87	1154.0
88	991.5
89	829.0
90	829.0
91	580.5
92	332.0
93	307.5
94	283.0
95	283.0
96	237.0
97	191.0
98	191.0
99	427.0
100	663.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12247980475773482
2	0.05764517516360867
3	0.012241503489800043
4	0.0022669450907037117
5	2.59079438937567E-4
6	3.886191584063506E-4
7	6.476985973439175E-5
8	4.5338901814074233E-4
9	3.886191584063506E-4
10	7.772383168127012E-4
11	0.0020078656517661447
12	0.002331714950438103
13	0.017034473110145033
14	0.009456399521221197
15	0.025907943893756703
16	0.01107564601458099
17	0.022345601608365157
18	0.005699747656626474
19	0.008355311905736537
20	0.006671295552642351
21	0.006800835272111134
22	0.007578073588923836
23	0.00958593924068998
24	0.01509137731811328
25	0.01768217170748895
26	0.02098543455394293
27	0.009780248819893155
28	0.006347446253970392
29	0.012111963770331259
30	0.0033680327061883716
31	0.006541755833173568
32	0.00699514485131431
33	0.008938240643346062
34	0.01327782124555031
35	0.018070790865895303
36	0.01586861563492598
37	0.011140415874315383
38	0.007254224290251877
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1543928.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.07436164998111
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.65957754706314	40.961472884204994
2	12.786869984497004	13.317361838879705
3	3.8100145930013776	5.952122334229778
4	1.609736278233577	3.353039564553196
5	0.8213781136449256	2.138637047066257
6	0.47636783273343164	1.4883930480107053
7	0.3330435488734356	1.214012114645991
8	0.23036880690389192	0.9597046850870344
9	0.16806593253441776	0.7876733536654735
>10	0.9096457566191892	8.707317405540135
>50	0.08683969893231851	3.214264269145613
>100	0.09248982169577119	10.153490778155241
>500	0.011483134756934665	3.9515874298481135
>1k	0.003994133828499014	3.4231578324294487
>5k	1.2481668214059418E-4	0.377765414538411
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5812	0.3764424247762849	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	4533	0.29360177417599786	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3998	0.2589498992180983	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	3589	0.23245902658673204	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2277	0.14748097061521004	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1802	0.11671528724137394	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1792	0.11606758864403004	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	1769	0.11457788187013902	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1707	0.11056215056660673	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1624	0.10518625220865221	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1605	0.10395562487369876	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1578	0.1022068386608702	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1572	0.10181821950246385	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1572	0.10181821950246385	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1565	0.1013648304843231	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.2384929867195877E-4
2	0.0	0.0	0.0	0.0	3.2384929867195877E-4
3	0.0	0.0	0.0	0.0	3.2384929867195877E-4
4	0.0	0.0	0.0	0.0	3.2384929867195877E-4
5	0.0	0.0	0.0	6.476985973439175E-5	3.886191584063506E-4
6	0.0	0.0	0.0	6.476985973439175E-5	3.886191584063506E-4
7	0.0	0.0	0.0	6.476985973439175E-5	3.886191584063506E-4
8	0.0	0.0	0.0	6.476985973439175E-5	3.886191584063506E-4
9	0.0	0.0	0.0	6.476985973439175E-5	3.886191584063506E-4
10	0.0	0.0	0.0	6.476985973439175E-5	3.886191584063506E-4
11	0.0	0.0	0.0	6.476985973439175E-5	3.886191584063506E-4
12	0.0	0.0	0.0	6.476985973439175E-5	9.067780362814847E-4
13	0.0	0.0	0.0	6.476985973439175E-5	9.715478960158764E-4
14	0.0	0.0	0.0	1.295397194687835E-4	9.715478960158764E-4
15	0.0	0.0	0.0	1.295397194687835E-4	0.0014249369141566188
16	0.0	0.0	0.0	1.943095792031753E-4	0.0014897067738910104
17	0.0	0.0	0.0	1.943095792031753E-4	0.0015544766336254023
18	0.0	0.0	0.0	3.2384929867195877E-4	0.0018135560725629693
19	0.0	0.0	0.0	3.2384929867195877E-4	0.0018135560725629693
20	0.0	0.0	0.0	3.886191584063506E-4	0.002137405371234928
21	0.0	0.0	0.0	5.18158877875134E-4	0.002137405371234928
22	0.0	0.0	0.0	5.829287376095258E-4	0.00220217523096932
23	0.0	0.0	0.0	0.0012306273349534434	0.00220217523096932
24	0.0	0.0	0.0	0.002137405371234928	0.00220217523096932
25	0.0	0.0	0.0	0.002331714950438103	0.00220217523096932
26	0.0	0.0	0.0	0.0036271121451259387	0.0022669450907037117
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGCAA	50	7.195263E-4	19.200874	13
CATCGTT	350	0.0	19.199009	28
TAGTACG	50	7.20782E-4	19.1959	4
TAATACC	90	4.378926E-7	17.773981	4
ATCGTTT	400	0.0	17.199112	29
CGTCGTA	300	0.0	17.06634	10
CGTTTAT	435	0.0	16.919216	31
ACCGTCG	320	0.0	16.498615	8
ATACCGT	350	0.0	16.454163	6
CGGCATC	405	0.0	16.19722	25
GTATAGA	100	1.3011922E-6	16.016294	1
CCGTCGT	320	0.0	15.999693	9
TACCGTC	335	0.0	15.75987	7
TATACTG	195	0.0	15.58591	5
TCTAGCG	380	0.0	15.578143	28
CTAGCGG	390	0.0	15.178703	29
GTCGTAG	340	0.0	15.059023	11
TAGCGGC	395	0.0	14.987054	30
CGCTTCG	395	0.0	14.987054	32
GGCGTTA	195	1.8189894E-12	14.768947	31
>>END_MODULE