FastQCFastQC Report
Thu 2 Feb 2017
SRR4062773_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062773_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1543928
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT63050.40837396562534No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT46400.30053214916757776No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA45710.29606302884590474No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC42790.2771502298034623No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT26810.1736479939479043No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19770.1280500126948925No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG19040.12332181293428192No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC18930.12260934447720359No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT18670.12092532812410942No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC17080.11062692042634113No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA17010.11017353140820038No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC16820.10894290407324693No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG16590.10745319729935593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG3700.020.32323329
TAGCGGC3900.019.28101730
TCTAGCG3950.019.03695328
GATATAC2300.018.0883311
TACCGTC4150.017.3524027
AAGACGG5000.017.2829915
CGTCGTA4000.017.20130510
ACCGTCG4050.016.9905958
CAAGACG5050.016.792814
CCGTCGT4100.016.7833929
ATACCGT4100.016.7833926
AACGAAT1354.4383341E-1016.59170331
GTTTTCG4600.016.34694928
GCGCAAG4950.016.1628441
TCGCGTA1800.016.0027719
GTACCGG2000.016.002776
GACGGAC5400.016.002777
TACCGGC1501.1459633E-1016.002777
GTATAGG2000.016.0012151
CGCAAGA5200.015.9991412
Produced by FastQC (version 0.11.5)