##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062773_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1543928
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16516767621288	32.0	32.0	32.0	32.0	32.0
2	31.32484416371748	32.0	32.0	32.0	32.0	32.0
3	31.405480695991006	32.0	32.0	32.0	32.0	32.0
4	31.50381429703976	32.0	32.0	32.0	32.0	32.0
5	31.431314154546065	32.0	32.0	32.0	32.0	32.0
6	34.955606738138044	36.0	36.0	36.0	36.0	36.0
7	34.98778569985129	36.0	36.0	36.0	36.0	36.0
8	34.92346469524485	36.0	36.0	36.0	36.0	36.0
9	35.027462420527385	36.0	36.0	36.0	36.0	36.0
10	34.888818001875734	36.0	36.0	36.0	32.0	36.0
11	35.048401868480916	36.0	36.0	36.0	36.0	36.0
12	34.955287422729555	36.0	36.0	36.0	36.0	36.0
13	35.002157484027755	36.0	36.0	36.0	36.0	36.0
14	34.945000025907945	36.0	36.0	36.0	32.0	36.0
15	34.925596271328715	36.0	36.0	36.0	32.0	36.0
16	34.9218435056557	36.0	36.0	36.0	36.0	36.0
17	34.89596146970584	36.0	36.0	36.0	32.0	36.0
18	34.89064451192024	36.0	36.0	36.0	32.0	36.0
19	34.884604074801416	36.0	36.0	36.0	32.0	36.0
20	34.86280189231622	36.0	36.0	36.0	32.0	36.0
21	34.84291106839179	36.0	36.0	36.0	32.0	36.0
22	34.827031441880706	36.0	36.0	36.0	32.0	36.0
23	34.76678640454736	36.0	36.0	36.0	32.0	36.0
24	34.747950681638	36.0	36.0	36.0	32.0	36.0
25	34.72938440134514	36.0	36.0	36.0	32.0	36.0
26	34.66146284023608	36.0	36.0	36.0	32.0	36.0
27	34.63571876408744	36.0	36.0	36.0	32.0	36.0
28	34.597246115103815	36.0	36.0	36.0	32.0	36.0
29	34.56669028607551	36.0	36.0	36.0	32.0	36.0
30	34.535594924115635	36.0	36.0	36.0	32.0	36.0
31	34.52932261089895	36.0	36.0	36.0	32.0	36.0
32	34.49613000088087	36.0	36.0	36.0	32.0	36.0
33	34.448546823427	36.0	36.0	36.0	32.0	36.0
34	34.429904762398245	36.0	36.0	36.0	32.0	36.0
35	34.383407127793525	36.0	36.0	36.0	32.0	36.0
36	34.35086480716717	36.0	36.0	36.0	32.0	36.0
37	34.32224494924634	36.0	36.0	36.0	32.0	36.0
38	33.89610655419165	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	7.0
21	29.0
22	100.0
23	374.0
24	989.0
25	2442.0
26	5275.0
27	10330.0
28	18274.0
29	28798.0
30	43273.0
31	62471.0
32	88061.0
33	140195.0
34	338804.0
35	804502.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.97124186732238	18.459850288773215	12.23127508466276	26.337632759241647
2	15.311530071350477	20.776551756299515	37.22809612883503	26.683822043514983
3	18.003559751491004	24.717927260856722	29.00413749863983	28.274375489012442
4	11.901339894956173	16.272854869433594	36.205727343326465	35.62007789228377
5	13.753046614250545	37.33518488892285	33.479497411470874	15.432271085355719
6	33.98101076022377	35.9252736690271	16.661085660138074	13.432629910611057
7	29.634852019726388	31.091985216869766	20.956110477083687	18.317052286320166
8	28.105038299261476	33.34963410700475	19.438057275952882	19.107270317780884
9	27.35855106126782	14.253744346155514	18.717552940322996	39.670151652253665
10	15.633608413956503	27.050652531136834	31.79976047842136	25.515978576485303
11	37.187452634524426	21.261382288129774	22.280914028989937	19.27025104835586
12	24.56945771329566	24.120796023122885	28.87225739592273	22.43748886765873
13	29.662839840018133	19.526855255274704	25.484981459591626	25.325323445115533
14	23.432180775269313	20.108515422998998	25.38376141892627	31.075542382805416
15	25.091195962506024	27.53386168266914	23.008067733728517	24.366874621096322
16	25.35942437692974	25.96003567526185	24.500769790281534	24.179770157526878
17	23.499282349954143	26.177516049971246	25.960731329440236	24.362470270634383
18	24.6655284443316	24.447124477307234	27.528615324030653	23.358731754330513
19	25.053046515122467	25.68707867206243	26.05982921483385	23.20004559798125
20	25.271126632848162	24.264279163277045	26.4158691337938	24.04872507008099
21	26.29570736920511	24.594977087617597	24.837152063733665	24.272163479443627
22	24.999303723556878	25.065887182489842	25.519665113700324	24.41514398025295
23	23.472852290656792	24.638842326435757	26.283418095709376	25.604887287198075
24	24.12042530480696	25.72386795239156	25.905158789788125	24.250547953013353
25	24.62142016985248	24.7367105201797	25.915781046784563	24.726088263183257
26	23.739837609007676	25.54536221896358	26.596706582172224	24.11809358985652
27	24.8633030339537	25.407111480731587	25.646760272189212	24.0828252131255
28	23.754281287728443	24.829201879880408	26.572417884771827	24.84409894761932
29	23.778634754988577	25.307656833738363	26.602017710670445	24.31169070060262
30	24.14005057230648	25.1815499168355	26.792376328429825	23.886023182428197
31	24.278787611857545	25.10661118912281	25.64083299221207	24.97376820680757
32	23.72150773870284	25.545880377841453	25.740449036483565	24.99216284697214
33	23.56029555782394	25.07331948121933	26.30310480799623	25.0632801529605
34	24.04548657709427	25.332787539315305	26.71976931566757	23.901956567922856
35	25.21918120534118	25.189127990424424	26.106463513842616	23.48522729039178
36	23.917242254820174	25.779310952324202	25.950562461461935	24.352884331393692
37	24.967355990693864	25.626842702509446	25.62781425040546	23.777987056391233
38	24.08305309574022	25.493999720194203	25.897580716199204	24.525366467866377
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	75.0
1	117.0
2	159.0
3	159.0
4	643.0
5	1127.0
6	1127.0
7	1523.0
8	1919.0
9	2067.5
10	2216.0
11	2216.0
12	2708.5
13	3201.0
14	4058.0
15	4915.0
16	4915.0
17	7305.5
18	9696.0
19	9696.0
20	11133.5
21	12571.0
22	13135.5
23	13700.0
24	13700.0
25	15854.0
26	18008.0
27	18008.0
28	23477.0
29	28946.0
30	35001.5
31	41057.0
32	41057.0
33	50991.5
34	60926.0
35	60926.0
36	66290.0
37	71654.0
38	80498.5
39	89343.0
40	89343.0
41	93825.5
42	98308.0
43	109099.0
44	119890.0
45	119890.0
46	130187.5
47	140485.0
48	140485.0
49	142635.5
50	144786.0
51	140768.0
52	136750.0
53	136750.0
54	129653.5
55	122557.0
56	122557.0
57	118193.0
58	113829.0
59	101757.5
60	89686.0
61	89686.0
62	84889.5
63	80093.0
64	65961.0
65	51829.0
66	51829.0
67	43069.5
68	34310.0
69	34310.0
70	27642.0
71	20974.0
72	16495.0
73	12016.0
74	12016.0
75	9046.0
76	6076.0
77	6076.0
78	5842.0
79	5608.0
80	4345.5
81	3083.0
82	3083.0
83	2926.5
84	2770.0
85	2770.0
86	1758.5
87	747.0
88	606.5
89	466.0
90	466.0
91	277.0
92	88.0
93	64.5
94	41.0
95	41.0
96	26.5
97	12.0
98	12.0
99	11.5
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010104098118565115
2	0.0
3	0.0
4	6.476985973439175E-5
5	6.476985973439175E-5
6	6.476985973439175E-5
7	3.886191584063506E-4
8	0.00220217523096932
9	0.004339580602204248
10	0.00110108761548466
11	0.005311128498220124
12	1.943095792031753E-4
13	1.943095792031753E-4
14	0.0
15	0.0
16	6.476985973439175E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	1.943095792031753E-4
22	6.476985973439175E-5
23	2.59079438937567E-4
24	0.0
25	0.0
26	0.0
27	1.295397194687835E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1543928.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.09516909038582
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.29386178939814	38.72049075988811
2	13.495903230802023	13.521591087490231
3	4.112529025848697	6.180535108170305
4	1.6978871140780518	3.402237683045088
5	0.890541058966773	2.230590246543587
6	0.5416468703937053	1.6280334935790581
7	0.3504621584410646	1.2289522760820704
8	0.251269036905654	1.0069891912773705
9	0.17727801637019758	0.799269498546591
>10	0.9795033890314605	8.881546215031861
>50	0.09081074049149067	3.223119718219035
>100	0.10101655278584248	10.699056865294326
>500	0.012610741347578348	4.240129043159871
>1k	0.004550267496548889	3.8268296205672505
>5k	1.3000764275853966E-4	0.4106291931053373
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	6305	0.40837396562534	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	4640	0.30053214916757776	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	4571	0.29606302884590474	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	4279	0.2771502298034623	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2681	0.1736479939479043	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1977	0.1280500126948925	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	1904	0.12332181293428192	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1893	0.12260934447720359	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1867	0.12092532812410942	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1708	0.11062692042634113	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1701	0.11017353140820038	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1682	0.10894290407324693	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1659	0.10745319729935593	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	6.476985973439175E-5	0.0
6	0.0	0.0	0.0	6.476985973439175E-5	6.476985973439175E-5
7	0.0	0.0	0.0	6.476985973439175E-5	6.476985973439175E-5
8	0.0	0.0	0.0	6.476985973439175E-5	6.476985973439175E-5
9	0.0	0.0	0.0	6.476985973439175E-5	6.476985973439175E-5
10	0.0	0.0	0.0	6.476985973439175E-5	1.943095792031753E-4
11	0.0	0.0	0.0	1.943095792031753E-4	1.943095792031753E-4
12	0.0	0.0	0.0	1.943095792031753E-4	5.18158877875134E-4
13	0.0	0.0	0.0	1.943095792031753E-4	9.067780362814847E-4
14	0.0	0.0	0.0	2.59079438937567E-4	9.067780362814847E-4
15	0.0	0.0	0.0	2.59079438937567E-4	0.001036317755750268
16	0.0	0.0	0.0	3.2384929867195877E-4	0.00110108761548466
17	0.0	0.0	0.0	3.2384929867195877E-4	0.0011658574752190516
18	0.0	0.0	0.0	4.5338901814074233E-4	0.0012306273349534434
19	0.0	0.0	0.0	4.5338901814074233E-4	0.0012306273349534434
20	0.0	0.0	0.0	5.829287376095258E-4	0.0014897067738910104
21	0.0	0.0	0.0	8.420081765470929E-4	0.001619246493359794
22	0.0	0.0	0.0	9.067780362814847E-4	0.0016840163530941858
23	0.0	0.0	0.0	0.0014897067738910104	0.0016840163530941858
24	0.0	0.0	0.0	0.00220217523096932	0.0017487862128285775
25	0.0	0.0	0.0	0.002396484810172495	0.0017487862128285775
26	0.0	0.0	0.0	0.003756651864594722	0.0019430957920317528
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	370	0.0	20.323233	29
TAGCGGC	390	0.0	19.281017	30
TCTAGCG	395	0.0	19.036953	28
GATATAC	230	0.0	18.088331	1
TACCGTC	415	0.0	17.352402	7
AAGACGG	500	0.0	17.282991	5
CGTCGTA	400	0.0	17.201305	10
ACCGTCG	405	0.0	16.990595	8
CAAGACG	505	0.0	16.79281	4
CCGTCGT	410	0.0	16.783392	9
ATACCGT	410	0.0	16.783392	6
AACGAAT	135	4.4383341E-10	16.591703	31
GTTTTCG	460	0.0	16.346949	28
GCGCAAG	495	0.0	16.162844	1
TCGCGTA	180	0.0	16.002771	9
GTACCGG	200	0.0	16.00277	6
GACGGAC	540	0.0	16.00277	7
TACCGGC	150	1.1459633E-10	16.00277	7
GTATAGG	200	0.0	16.001215	1
CGCAAGA	520	0.0	15.999141	2
>>END_MODULE