FastQCFastQC Report
Thu 2 Feb 2017
SRR4062772_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062772_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1838113
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT34810.18937899900604585No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT23530.12801171636346623No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA20580.11196264865108946No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG18870.10265962973984732No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT18480.10053788858465176No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG18430.10026587048783181No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG4450.015.81917728
GTATCAA26950.015.2759911
GTATTAG4750.015.1758821
CTAGCGG4700.014.9777329
TATAACG756.2255503E-414.9400652
TAACCTA756.257763E-414.9299065
ATCGTTT4100.014.82828629
GTCCTAT5100.014.7625951
TAGCGGC4850.014.51489630
CATCGTT4200.014.47522928
TATACAG2550.014.4280615
ACCGTCG2950.014.1003768
TAAACGC3950.013.77123828
CGTTATT2700.013.6357752
GTCCTAG2600.013.5545271
GTATAGA2500.013.4559481
TCGTTTA4300.013.39482530
CGCCCCT3250.013.2892575
AGCGGCG5800.013.24086131
ATACCGT3400.013.1738046
Produced by FastQC (version 0.11.5)