##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062772_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1838113
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28634637805184	32.0	32.0	32.0	32.0	32.0
2	30.9426634815161	32.0	32.0	32.0	32.0	32.0
3	30.982793223267556	32.0	32.0	32.0	32.0	32.0
4	31.04871517692329	32.0	32.0	32.0	32.0	32.0
5	30.974514624508938	32.0	32.0	32.0	32.0	32.0
6	34.62912345432517	36.0	36.0	36.0	32.0	36.0
7	34.570180940997645	36.0	36.0	36.0	32.0	36.0
8	34.55918161723463	36.0	36.0	36.0	32.0	36.0
9	34.67951589483345	36.0	36.0	36.0	32.0	36.0
10	34.43603032022514	36.0	36.0	36.0	32.0	36.0
11	34.64790575987439	36.0	36.0	36.0	32.0	36.0
12	34.50409360033904	36.0	36.0	36.0	32.0	36.0
13	34.57116510247194	36.0	36.0	36.0	32.0	36.0
14	34.48782256585966	36.0	36.0	36.0	32.0	36.0
15	34.43806664769794	36.0	36.0	36.0	32.0	36.0
16	34.44291618632804	36.0	36.0	36.0	32.0	36.0
17	34.383538988081796	36.0	36.0	36.0	32.0	36.0
18	34.40387451696387	36.0	36.0	36.0	32.0	36.0
19	34.38977364286091	36.0	36.0	36.0	32.0	36.0
20	34.378391861653775	36.0	36.0	36.0	32.0	36.0
21	34.36288302188168	36.0	36.0	36.0	32.0	36.0
22	34.342105191574184	36.0	36.0	36.0	32.0	36.0
23	34.29687619857974	36.0	36.0	36.0	32.0	36.0
24	34.27155566605535	36.0	36.0	36.0	32.0	36.0
25	34.26091486214395	36.0	36.0	36.0	32.0	36.0
26	34.20842516210919	36.0	36.0	36.0	32.0	36.0
27	34.21294827902311	36.0	36.0	36.0	32.0	36.0
28	34.206308861315925	36.0	36.0	36.0	32.0	36.0
29	34.169852451943925	36.0	36.0	36.0	32.0	36.0
30	34.13707427127712	36.0	36.0	36.0	32.0	36.0
31	34.16403942521488	36.0	36.0	36.0	32.0	36.0
32	34.11874841209436	36.0	36.0	36.0	32.0	36.0
33	34.07996243974119	36.0	36.0	36.0	32.0	36.0
34	34.08692773512836	36.0	36.0	36.0	32.0	36.0
35	34.064256658867	36.0	36.0	36.0	32.0	36.0
36	34.01818985013435	36.0	36.0	36.0	32.0	36.0
37	34.019586935079616	36.0	36.0	36.0	32.0	36.0
38	33.43745460698009	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	6.0
8	5.0
9	4.0
10	14.0
11	21.0
12	7.0
13	14.0
14	230.0
15	427.0
16	580.0
17	706.0
18	830.0
19	1117.0
20	1449.0
21	2089.0
22	3091.0
23	4714.0
24	7094.0
25	10833.0
26	15779.0
27	23240.0
28	32972.0
29	44046.0
30	60066.0
31	81127.0
32	113036.0
33	170443.0
34	390190.0
35	873983.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.27712898769865	17.079398833049368	11.337189034672988	26.306283144578995
2	16.861568306837814	19.4963873705479	37.34501944843308	26.29702487418121
3	18.812036874312742	23.40369733377804	28.68162307479274	29.102642717116478
4	12.782369265906555	15.228857770381103	35.76433451954585	36.224438444166495
5	14.88551549445653	36.15498319196522	33.66040860471278	15.29909270886547
6	34.590821327788504	35.2747851188368	16.638512732378764	13.495880820995925
7	30.448726492876116	30.285298020306694	20.93745052670864	18.328524960108545
8	28.133866128431183	32.86988501745004	19.473316268657122	19.522932585461657
9	27.611039388850173	14.008364030642362	18.39292446748261	39.98767211302485
10	15.937546583276482	26.385529452599577	31.79033200479189	25.88659195933205
11	37.61668489230552	20.90682074132106	22.272057103374735	19.204437262998688
12	24.82076230290382	23.449535434604492	28.72846508986095	23.001237172630738
13	29.702735408831316	19.122233685053626	25.012025090541854	26.163005815573197
14	23.646023765996343	19.461674066335856	25.072418821276226	31.819883346391574
15	25.273936523346098	26.95085903098084	22.078826676650422	25.696377769022643
16	25.91341356319162	25.494671595517893	23.510979462273504	25.080935379016978
17	24.30125220654334	25.733083893267512	25.003210506955377	24.962453393233773
18	25.25098041776025	24.55832234307875	25.75272362447715	24.437973614683848
19	25.830064762053993	24.761324926181704	25.192400918627673	24.216209393136626
20	25.94871454477099	24.11007259572565	24.731078076177905	25.210134783325454
21	26.96547296488536	24.05559364084484	24.32142896005397	24.657504434215824
22	25.944307819539052	24.070654421206118	24.705760734727633	25.27927702452719
23	24.68339071729223	23.863547329795985	25.273244856119337	26.179817096792448
24	25.094172794767783	24.749584431766554	24.969135046753127	25.187107726712537
25	25.155867235613368	24.20031320363351	25.12539572158178	25.51842383917135
26	24.953407268629338	25.04743616183635	25.34922102394635	24.64993554558796
27	25.563013861858046	24.533753082967113	24.546049585373908	25.35718346980093
28	24.765097165714362	24.46999282913872	25.418254901811434	25.346655103335486
29	24.816380407453494	24.8556098988902	25.168520863179396	25.159488830476906
30	24.772897784553077	24.852547456569436	25.448559858850334	24.92599490002715
31	25.421315532757742	24.63790667320278	24.394380174462036	25.546397619577444
32	25.01021507979732	24.560316305661303	24.407811013134605	26.021657601406773
33	24.70148612194753	24.34461880651034	25.02043050418458	25.93346456735755
34	25.492354393958607	24.323816975568775	25.171382041980507	25.012446588492114
35	25.935174171530306	24.27910540182589	25.121314451192905	24.664405975450894
36	24.88101411289335	24.950226436386963	24.770774987580417	25.397984463139267
37	25.733487785224092	24.687128821691314	24.66879264724846	24.910590745836135
38	24.899237206063177	24.606361331461713	25.11398383007432	25.380417632400786
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	227.0
1	217.0
2	207.0
3	207.0
4	501.5
5	796.0
6	796.0
7	924.5
8	1053.0
9	1012.5
10	972.0
11	972.0
12	1397.0
13	1822.0
14	2467.5
15	3113.0
16	3113.0
17	4845.0
18	6577.0
19	6577.0
20	8084.0
21	9591.0
22	10747.0
23	11903.0
24	11903.0
25	14121.5
26	16340.0
27	16340.0
28	21030.0
29	25720.0
30	31654.0
31	37588.0
32	37588.0
33	48536.5
34	59485.0
35	59485.0
36	67129.5
37	74774.0
38	85700.5
39	96627.0
40	96627.0
41	105296.0
42	113965.0
43	128178.5
44	142392.0
45	142392.0
46	147535.5
47	152679.0
48	152679.0
49	163560.0
50	174441.0
51	177190.5
52	179940.0
53	179940.0
54	172654.5
55	165369.0
56	165369.0
57	159934.5
58	154500.0
59	138695.0
60	122890.0
61	122890.0
62	113873.5
63	104857.0
64	86681.5
65	68506.0
66	68506.0
67	56447.0
68	44388.0
69	44388.0
70	35440.5
71	26493.0
72	20676.0
73	14859.0
74	14859.0
75	11166.5
76	7474.0
77	7474.0
78	7154.0
79	6834.0
80	5314.5
81	3795.0
82	3795.0
83	3855.0
84	3915.0
85	3915.0
86	2628.0
87	1341.0
88	1156.5
89	972.0
90	972.0
91	695.5
92	419.0
93	349.0
94	279.0
95	279.0
96	271.5
97	264.0
98	264.0
99	505.0
100	746.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12061282412996373
2	0.05152022753769763
3	0.010554302156613876
4	0.0027201809681994525
5	2.176144774559562E-4
6	2.176144774559562E-4
7	0.0
8	4.352289549119124E-4
9	2.176144774559562E-4
10	3.808253355479233E-4
11	0.002339355632651529
12	0.0027745845875634416
13	0.015722645996192837
14	0.010499898537249886
15	0.025080068526798952
16	0.012349621595625514
17	0.02159823688750365
18	0.006582837943042675
19	0.008378157382054312
20	0.00685485603986262
21	0.007235681375410544
22	0.008323753762690324
23	0.010935127492161799
24	0.014580169989549065
25	0.018007598009480374
26	0.020673375358315836
27	0.010445494917885897
28	0.006474030704314696
29	0.0114247600664377
30	0.0034818316392952993
31	0.006528434323678686
32	0.007834121188414422
33	0.009520633388698083
34	0.014144941034637152
35	0.017789983532024416
36	0.015777049615556822
37	0.011751181782621634
38	0.007235681375410544
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1838113.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.84214614626452
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.72950268257748	45.88838189798323
2	11.597949738491083	13.18504708064681
3	3.3102651314899054	5.644877231610982
4	1.4048418396141047	3.1941690063892816
5	0.770424527992616	2.1896291807411568
6	0.4909266959225051	1.6743196198037875
7	0.31718247739607297	1.2620532914627274
8	0.22706640369941056	1.0325553363190874
9	0.16407890447522358	0.8393937360929671
>10	0.8513669727835577	8.542191686629135
>50	0.06207021520860447	2.481840418007482
>100	0.06188968495914334	7.593842175248443
>500	0.008964569467469534	3.498138854016078
>1k	0.003470155922891433	2.9735604850487527
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3481	0.18937899900604585	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2353	0.12801171636346623	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2058	0.11196264865108946	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1887	0.10265962973984732	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1848	0.10053788858465176	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1843	0.10026587048783181	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.0880723872797809E-4	0.0	0.0	0.0
6	0.0	1.0880723872797809E-4	0.0	0.0	5.4403619363989045E-5
7	0.0	1.0880723872797809E-4	0.0	5.4403619363989045E-5	5.4403619363989045E-5
8	0.0	1.0880723872797809E-4	0.0	5.4403619363989045E-5	5.4403619363989045E-5
9	0.0	1.0880723872797809E-4	0.0	5.4403619363989045E-5	2.1761447745595618E-4
10	0.0	1.0880723872797809E-4	0.0	5.4403619363989045E-5	2.1761447745595618E-4
11	0.0	1.0880723872797809E-4	0.0	5.4403619363989045E-5	2.1761447745595618E-4
12	0.0	1.0880723872797809E-4	0.0	5.4403619363989045E-5	4.3522895491191236E-4
13	0.0	1.0880723872797809E-4	0.0	5.4403619363989045E-5	4.896325742759014E-4
14	0.0	1.0880723872797809E-4	0.0	5.4403619363989045E-5	4.896325742759014E-4
15	0.0	1.0880723872797809E-4	0.0	5.4403619363989045E-5	8.704579098238247E-4
16	0.0	1.0880723872797809E-4	0.0	1.0880723872797809E-4	8.704579098238247E-4
17	0.0	1.0880723872797809E-4	0.0	1.6321085809196714E-4	8.704579098238247E-4
18	0.0	1.0880723872797809E-4	0.0	2.1761447745595618E-4	8.704579098238247E-4
19	0.0	1.6321085809196714E-4	0.0	2.1761447745595618E-4	8.704579098238247E-4
20	0.0	1.6321085809196714E-4	0.0	2.1761447745595618E-4	9.248615291878138E-4
21	0.0	1.6321085809196714E-4	0.0	2.7201809681994525E-4	9.248615291878138E-4
22	0.0	1.6321085809196714E-4	0.0	3.808253355479233E-4	9.248615291878138E-4
23	0.0	1.6321085809196714E-4	0.0	4.896325742759014E-4	0.001088072387279781
24	0.0	1.6321085809196714E-4	0.0	8.704579098238247E-4	0.001088072387279781
25	0.0	1.6321085809196714E-4	0.0	9.792651485518028E-4	0.001088072387279781
26	0.0	1.6321085809196714E-4	0.0	0.001251283245371748	0.001088072387279781
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	445	0.0	15.819177	28
GTATCAA	2695	0.0	15.275991	1
GTATTAG	475	0.0	15.175882	1
CTAGCGG	470	0.0	14.97773	29
TATAACG	75	6.2255503E-4	14.940065	2
TAACCTA	75	6.257763E-4	14.929906	5
ATCGTTT	410	0.0	14.828286	29
GTCCTAT	510	0.0	14.762595	1
TAGCGGC	485	0.0	14.514896	30
CATCGTT	420	0.0	14.475229	28
TATACAG	255	0.0	14.428061	5
ACCGTCG	295	0.0	14.100376	8
TAAACGC	395	0.0	13.771238	28
CGTTATT	270	0.0	13.635775	2
GTCCTAG	260	0.0	13.554527	1
GTATAGA	250	0.0	13.455948	1
TCGTTTA	430	0.0	13.394825	30
CGCCCCT	325	0.0	13.289257	5
AGCGGCG	580	0.0	13.240861	31
ATACCGT	340	0.0	13.173804	6
>>END_MODULE